System and method for selecting and culturing cells

Patent metadata

Field	Value
Publication number	US-10449283-B2
Application number	US-201715498918-A
Country	US
Kind code	B2
Filing date	Apr 27, 2017
Priority date	Apr 29, 2016
Publication date	Oct 22, 2019
Grant date	Oct 22, 2019

Abstract

A cell processing system includes at least one processor connectable to a source container filled with a biological fluid, the at least one processor including a spinning membrane configured to receive and separate target cells from the biological fluid, the target cells exiting at a first outlet, one or more containers selectively connected to the first outlet; and, and a magnet. The system also includes a controller coupled to the at least one processor and configured to operate the spinning membrane to receive biological fluid from the source container and to direct the target cells to one of the one or more containers, to pause to permit magnetic particles to be associated with the target cells, and to operate the spinning membrane to receive the contents of one of the one or more containers with the magnet applied to the target cells associated with the magnetic particles.

First claim

The invention claimed is:

1. A cell processing system comprising:
at least one processor connectable to a source container filled with a biological fluid, the at least one processor comprising:
a spinning membrane configured to receive and separate target cells from the biological fluid, the target cells exiting at a first outlet;
one or more containers selectively connected to the first outlet; and
a magnet;

a controller coupled to the at least one processor and configured:
to operate the spinning membrane to receive biological fluid from the source container and to direct the target cells to one of the one or more containers,
to pause operation of the processor to permit magnetic particles to be associated with the target cells in the one of the one or more containers,
to transfer the target cells associated with the magnetic particles from the one of the one or more containers to another of the one or more containers;
to operate the spinning membrane to receive the contents of the another of the one or more containers with the magnet applied to the target cells associated with the magnetic particles,
to deactivate the magnet applied to the target cells associated with the magnetic particles in the another of the one or more containers,
to prompt an operator to remove the another of the one or more containers.

2. The cell processing system according to claim 1 , wherein the controller is configured to pause operation of the processor to permit a conjugated antibody bead to be added to the one of the one or more containers and to permit the conjugated antibody bead to form conjugated antibody bead-target cell complexes with the target cells.

3. The cell processing system according to claim 2 , wherein the controller is configured to operate the spinning membrane to mix the conjugated antibody beads with the target cells.

4. The cell processing system according to claim 1 , wherein the controller is configured to pause operation of the processor to permit monoclonal antibodies to be added to the target cells to associate the monoclonal antibodies with the target cells, and to permit the magnetic particles to be added to the target cells associated with the monoclonal antibodies to associate the magnetic particles with the target cells.

5. The cell processing system according to claim 4 , wherein the controller is configured to operate the spinning membrane to mix the monoclonal antibodies with the target cells, and wherein the controller is configured to operate the spinning membrane to mix the magnetic particles with the target cells associated with the monoclonal antibodies.

6. The cell processing system according to claim 1 , further comprising:
a magnetic separator having first and second opposing plates, the magnet associated with the first plate,
at least the first plate translatable relative to the second plate, and
the another of the one or more containers disposed between the first and second plates.

7. The cell processing system according to claim 6 , wherein the first and second plates are mounted on an axle, the first and second plates being pivotable about the axle to change the inclination of the first and second plates and the another of the one or more containers disposed between the first and second plates.

8. The cell processing system according to claim 1 , wherein the at least one processor comprises reusable hardware, and a disposable circuit, the disposable circuit comprising the spinning membrane and the one or more containers.

9. The cell processing system according to claim 8 , wherein the disposable circuit is a closed circuit.

Assignees

• Fenwal Inc

Inventors

• Wegener Christopher J
• Olson Bret M
• Schlinker Alaina
• Binninger Steven
• Kadakia Avnie A
• Thompson Kyle
• Wolok Kyle
• Hamilton Melanie

Classifications

• C12M47/04 Primary

  Cell isolation or sorting (purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04) · CPC title

• C12M33/14

  with filters, sieves or membranes · CPC title

• A61M1/362

  changing physical properties of target cells by binding them to added particles to facilitate their subsequent separation from other cells, e.g. immunoaffinity · CPC title

• C12M23/28

  disposable or single use · CPC title

• G01N33/54366

  Apparatus specially adapted for solid-phase testing · CPC title