Self-assembled peptide nucleic acids

Patent metadata

Field	Value
Publication number	US-10446768-B2
Application number	US-201715648524-A
Country	US
Kind code	B2
Filing date	Jul 13, 2017
Priority date	May 2, 2013
Publication date	Oct 15, 2019
Grant date	Oct 15, 2019

Abstract

Ordered (e.g., self-assembled) structures, arranged from peptide nucleic acids and/or analogs thereof, are disclosed. The peptide nucleic acids forming the ordered structures comprise from 1 to 10 PNA backbone units, at least one comprising a guanine nucleobase or an analog thereof. Processes of generating the ordered structures, uses thereof and articles-of manufacturing, devices and systems containing same are also disclosed.

First claim

What is claimed is:

1. A process of generating a composition-of-matter comprising a plurality of peptide nucleic acids arranged in an ordered nanometric or micrometric structure, each of said peptide nucleic acids independently comprising 1 to 10 backbone units, at least one of said backbone units comprising a guanine nucleobase or an analog thereof, the process comprising contacting said plurality of peptide nucleic acids with an aqueous solution under conditions which favor formation of said ordered nanometric or micrometric structure, said aqueous solution having a pH greater than 8.

2. The process of claim 1 , wherein said contacting is such that a concentration of said peptide nucleic acids in said aqueous solution ranges from 10 mg/ml to 100 mg/ml.

3. The process of claim 2 , wherein said concentration is about 50 mg/ml.

4. The process of claim 1 , wherein each of said peptide nucleic acids independently comprises 2 to 6 of said backbone units.

5. The process of claim 1 , wherein each of said peptide nucleic acids comprises 2 of said backbone units and is being a peptide nucleic acid dimer (PNA dimer).

6. The process of claim 5 , wherein each of said peptide nucleic acid dimers is independently selected from the group consisting of AG, CG, GG, GA, GC, and GT.

7. The process of claim 5 , wherein each of said peptide nucleic acid dimers is AG, and wherein said ordered structure is a ribbon-shaped micrometric structure.

8. The process of claim 5 , wherein each of said peptide nucleic acid dimers is CG, and wherein said ordered structure is a fibrillar micrometric structure.

9. The process of claim 5 , wherein each of said peptide nucleic acid dimers is GG, and wherein said ordered structure is a clustered spherical micrometric structure.

10. The process of claim 5 , wherein each of said peptide nucleic acid dimers is GA, and wherein said ordered structure is a micrometric or nanometric folded sheet structure.

11. The process of claim 5 , wherein each of said peptide nucleic acid dimers is GC, and wherein said ordered structure is a fibrillar micrometric structure.

12. The process of claim 5 , wherein each of said peptide nucleic acid dimers is GT, and wherein said ordered structure is a fractal porous nanometric or micrometric structure.

13. The process of claim 1 , wherein said aqueous solution comprises a buffer having pH greater than 8.

14. The process of claim 1 , wherein said aqueous solution comprises a polyamine.

15. The process of claim 14 , wherein a concentration of said polyamine in said aqueous solution ranges from 0.1% to 10%, by volume.

16. The process of claim 1 , wherein said ordered structure is formed at an elongation rate of at least 1 micron per second.

17. The process of claim 1 , wherein at least 10 9 molecules of said peptide nucleic acids organize into said ordered nanometric or micrometric structure per second.

Assignees

• Univ Ramot

Inventors

• Berger Or
• Adler-Abramovich Lihi
• Gazit Ehud

Classifications

• C07K14/003 Primary

  Peptide-nucleic acids (PNAs) · CPC title

• C09K11/06

  containing organic luminescent materials · CPC title

• C09K2211/145

  containing oxygen as the only heteroatom · CPC title

• C09K2211/1466

  containing nitrogen as the only heteroatom · CPC title

• H01L51/0093 Primary

  Electricity · mapped topic