Fermentation processes with reduced foaming

Patent metadata

Field	Value
Publication number	US-10407698-B2
Application number	US-201414900085-A
Country	US
Kind code	B2
Filing date	Jun 19, 2014
Priority date	Jun 20, 2013
Publication date	Sep 10, 2019
Grant date	Sep 10, 2019

Abstract

The present invention relates to processes of producing a fermentation product from readily fermentable sugar-material in a fermentation vat comprising a fermentation medium, comprising: feeding the readily fermentable sugar-material into the fermentation vat comprising a slurry of fermenting organism; fermenting the readily fermentable sugar material into a desired fermentation product, wherein protease is added during or after feeding of the readily fermentable sugar-material into fermentation vat or during fermentation of the readily fermentable sugar-material into the desired fermentation product. The invention also related to the use of protease for reducing foaming in the fermentation yells generating by the fermenting organism during fermentation of the readily fermentable sugar-material.

First claim

The invention claimed is:

1. A process of producing a fermentation product from readily fermentable sugar-material in a fermentation vat comprising a fermentation medium using a fermenting organism, the method comprising:
i) feeding the readily fermentable sugar material into the fermentation vat comprising a slurry of fermenting organism;
ii) fermenting the readily fermentable sugar material into a desired fermentation product,
wherein a protease is added
a) before, during and/or after feeding in step i), and/or
b) during fermentation in step ii);
wherein the fermentation product is ethanol;
wherein the readily fermentable sugar-material comprises sugar cane juice, sugar cane molasses, sweet sorghum or sugar beets;
wherein the fermenting organism is Saccharomyces cerevisiae ; and
wherein the protease is the Pyrocuccus furiousus protease of SEQ ID NO: 2, or a protease having at least 95% sequence identity to the protease of SEQ ID NO: 2; or the Meripilus giganteus protease of SEQ ID NO: 7, or a protease having at least 95% sequence identity to the protease of SEQ ID NO: 7.

2. The process of claim 1 , wherein the readily fermentable sugar material is feed into the fermentation vat as a feeding stream.

3. The process of claim 2 , wherein the protease is mixed with the feeding stream of the readily fermentable sugar-material.

4. The process of claim 1 , wherein the protease is mixed with the feeding stream of readily fermentable sugar-material before feeding step i).

5. The process of claim 1 , wherein the fermenting organism is a strain of Saccharomyces cerevisae generating foam when fermented.

6. The process of claim 1 , wherein the fermenting organism is recycled after fermentation in step ii).

7. The process of claim 5 , wherein the fermenting organism is collected after fermentation in step ii), acid washed, and recycled to the fermentation vat.

8. The process of claim 1 , wherein the readily fermentable sugar-material substrate does not contain starch or cellulose/hemicellulose.

9. The process of claim 1 , wherein the fermentation product is produced from readily fermentable sugar material by fermentation in a fermentation vat, the process comprising adding the protease to the readily fermentable sugar material before feeding; feeding the protease-containing readily fermentable sugar material into the fermentation vat comprising a slurry of fermenting organism; and fermenting the readily fermentable sugar material into the fermentation product.

10. The process of claim 1 , wherein ethanol is produced in a batch, fed batch, semi-continuous or continuous fermentation process in a fermentation vat comprising sugar cane molasses, comprising adding the protease to the sugar cane molasses before feeding; feeding the protease-containing sugar cane molasses into the fermentation vat comprising a slurry of Saccharomyces cerevisae yeast; and fermenting the sugar cane molasses into ethanol.

11. The process of claim 1 , wherein the fermentation product is produced from readily fermentable sugar material by fermentation in a fermentation vat, wherein the process comprises: feeding the readily fermentable sugar material into the fermentation vat comprising a slurry of fermenting organism; feeding the protease into the fermentation vat comprising a slurry of readily fermentable sugars and fermenting organism before fermentation; and fermenting the readily fermentable sugar material into the fermentation product.

12. The process of claim 1 , wherein ethanol is produced in a batch or fed batch fermentation process in a fermentation vat comprising sugar cane molasses, wherein the process comprises: feeding sugar cane molasses into the fermentation vat comprising a slurry of Saccharomyces cerevisae yeast; feeding the protease into the fermentation vat comprising a slurry of Saccharomyces cerevisae yeast and the sugar cane molasses before fermentation; and fermenting the sugar cane molasses into ethanol.

13. The process of claim 1 wherein the fermentation product is produced from readily fermentable sugar material by fermentation in a fermentation vat, wherein the process comprises: feeding the readily fermentable sugar material into the fermentation vat comprising a slurry of fermenting organism; and adding the protease into the fermentation vat during fermentation of the readily fermentable sugar-material into the desired fermentation product.

14. The process of claim 1 , wherein ethanol is produced as a batch, fed batch, semi-continuous or continuous fermentation process in a fermentation vat comprising sugar cane molasses, wherein the process comprises: feeding the sugar cane molasses into the fermentation vat comprising a slurry of Saccharomyces cerevisae yeast; adding the protease into the fermentation vat during fermentation of the sugar cane molasses into ethanol.

15. The process of claim 1 , comprising
i) feeding the readily fermentable sugar material into the fermentation vat comprising a slurry of fermenting organism;
ii) fermenting the readily fermentable sugar material into a desired fermentation product,

wherein feeding of the readily fermentable sugar-material is done by introducing a feeding stream into the fermentation vat; wherein
protease is mixed with the feeding stream before in step i); or
protease is added to fermentation vat after feeding.

16. The process of claim 1 , wherein the protease is capable of hydrolyzing mannoprotain in fermented readily fermentable sugar material.

Assignees

• Novozymes As

Inventors

• De Oliveira Lino Felipe Senne
• Basso Thiago O
• Nymand-Grarup Soren
• Do Nascimento Camila

Classifications

• C12N9/52

  derived from bacteria {or Archaea} · CPC title

• C12N1/34

  Processes using foam culture · CPC title

• C12P7/06 Primary

  Ethanol, i.e. non-beverage · CPC title

• Y02E50/17

  Cross-Sectional Technologies · mapped topic

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title