Maize event DP-004114-3 and methods for detection thereof

Patent metadata

Field	Value
Publication number	US-10407688-B2
Application number	US-201715648112-A
Country	US
Kind code	B2
Filing date	Jul 12, 2017
Priority date	Apr 24, 2012
Publication date	Sep 10, 2019
Grant date	Sep 10, 2019

Abstract

The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-004114-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

First claim

What is claimed is:

1. A pair of polynucleotide primers comprising a first polynucleotide primer and a second polynucleotide primer, wherein the first polynucleotide primer comprises the nucleotide sequence selected from SEQ ID NO: 29 and the complement thereof; and the second polynucleotide primer comprises the nucleotide sequence selected from SEQ ID NO: 30 and the complement thereof, which function together in the presence of a DP-004114-3 DNA template in a sample to produce an amplicon diagnostic for event DP-004114-3, wherein a detectable label or reporter molecule is attached to the first and/or second polynucleotide primer.

2. A kit for detecting nucleic acids that are unique to event DP-004114-3 comprising a primer pair selected from the group consisting of SEQ ID NOs: 29 and 30, SEQ ID NOs: 33 and 34, and the complements thereof, wherein a detectable label or reporter molecule is attached to the first and/or second polynucleotide primer, and which upon amplification of or hybridization to a target nucleic acid sequence in a sample followed by detection of the amplicon or hybridization to the target sequence, are diagnostic for the presence of nucleic acid sequences unique to event DP-004114-3 in the sample.

Assignees

• Pioneer Hi Bred Int
• Du Pont

Inventors

• Alarcon Clara Maria
• Harmon Matthew C
• Pascual M Alejandra
• Register Iii James C
• Scelonge Christopher J
• Young Joshua K
• Zhong Cathy Xiaoyan

Classifications

• C12Q1/6895 Primary

  for plants, fungi or algae · CPC title

• C12N15/8286

  for insect resistance · CPC title

• C12N15/8241 Primary

  Phenotypically and genetically modified plants via recombinant DNA technology · CPC title

• Y02A40/162

  Cross-Sectional Technologies · mapped topic

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title