Maize event DP-004114-3 and methods for detection thereof

Patent metadata

Field	Value
Publication number	US-9725772-B2
Application number	US-201313853413-A
Country	US
Kind code	B2
Filing date	Mar 29, 2013
Priority date	Apr 24, 2012
Publication date	Aug 8, 2017
Grant date	Aug 8, 2017

Abstract

The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-004114-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

First claim

What is claimed is:

1. An amplicon comprising the nucleic acid sequence selected from the group consisting of SEQ ID NO: 32, SEQ ID NO: 35 and full length complements thereof.

2. A biological sample derived from corn event DP-004114-3plant, tissue, or seed, wherein said sample comprises a nucleotide sequence which is or is complementary to a sequence selected from the group consisting of SEQ ID NO: 32 and SEQ ID NO: 35, wherein said nucleotide sequence is detectable in said sample using a nucleic acid amplification or nucleic acid hybridization method, wherein a representative sample of said corn event DP-004114-3 seed of has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-11506.

3. The biological sample of claim 2 , wherein said biological sample comprises plant, tissue, or seed of transgenic corn event DP-004114-3.

4. The biological sample of claim 3 , wherein said biological sample is selected from the group consisting of corn flour, corn meal, corn syrup, corn oil, corn starch, and cereals manufactured in whole or in part to contain corn by-products.

5. An extract derived from corn event DP-004114-3 plant, tissue, or seed and comprising a nucleotide sequence which is or is complementary to a sequence selected from the group consisting of SEQ ID NO: 32 and SEQ ID NO: 35, wherein a representative sample of said corn event DP-004114-3 seed has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-11506.

6. The extract of claim 5 , wherein said extract comprises plant, tissue, or seed of transgenic corn plant event DP-004114-3.

7. The extract of claim 6 , further comprising a composition selected from the group consisting of corn flour, corn meal, corn syrup, corn oil, corn starch, and cereals manufactured in whole or in part to contain corn by-products, wherein said composition comprises a detectable amount of said nucleotide sequence.

Assignees

• Pioneer Hi Bred Int
• Du Pont
• Pioneer Hi Bred Int
• Du Pont

Inventors

• Alarcon Clara Maria
• Harmon Matthew C
• Pascual M Alejandra
• Register Iii James C
• Scelonge Christopher J
• Young Joshua K
• Zhong Cathy Xiaoyan

Classifications

• C12Q1/6895 Primary

  for plants, fungi or algae · CPC title

• C12N15/8241 Primary

  Phenotypically and genetically modified plants via recombinant DNA technology · CPC title

• C12N15/8286

  for insect resistance · CPC title

• C12Q2535/125

  Allele specific primer extension · CPC title

• C07K14/325

  Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title