Penicillin-G acylases

Patent metadata

Field	Value
Publication number	US-10400231-B2
Application number	US-201815914258-A
Country	US
Kind code	B2
Filing date	Mar 7, 2018
Priority date	May 7, 2015
Publication date	Sep 3, 2019
Grant date	Sep 3, 2019

Abstract

The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.

First claim

What is claimed is:

1. A method for producing free insulin comprising
i) providing a protein comprising the penicillin G acylase of SEQ ID NO: 12, said penicillin G acylase capable of removing the A1/B1/B29 tri-phenyl acetate protecting groups from insulin to produce free insulin; and
ii) exposing said protein comprising the penicillin G acylase of SEQ ID NO: 12 to insulin comprising A1/B1/B29 tri-phenyl acetate protecting groups, under conditions such that said penicillin G acylase removes the A1/B1/B29 tri-phenyl acetate protecting groups and free insulin is produced.

2. The method of claim 1 , wherein the penicillin G acylase produces more free insulin, as compared to the penicillin G acylase of SEQ ID NO: 2.

Assignees

• Codexis Inc

Inventors

• Banerjee Goutami
• Yang Jie
• Zhang Xiyun
• Milczek Erika M
• Mayo Melissa Ann
• Jenne Stephan

Classifications

• A61P5/50

  for increasing or potentiating the activity of insulin · CPC title

• A61P3/10

  for hyperglycaemia, e.g. antidiabetics · CPC title

• C12N9/84 Primary

  Penicillin amidase {(3.5.1.11)} · CPC title

• C12Y305/01011

  Penicillin amidase (3.5.1.11), i.e. penicillin-amidohydrolase · CPC title

• C12P21/00

  Preparation of peptides or proteins (single cell protein C12N1/00) · CPC title