Penicillin-G acylases

Patent metadata

Field	Value
Publication number	US-9944916-B2
Application number	US-201615148056-A
Country	US
Kind code	B2
Filing date	May 6, 2016
Priority date	May 7, 2015
Publication date	Apr 17, 2018
Grant date	Apr 17, 2018

Abstract

The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.

First claim

What is claimed is:

1. An engineered non-naturally occurring penicillin G acylase capable of removing the A1/B1/B29 tri-phenyl acetate protecting groups from insulin to produce free insulin, wherein said penicillin G acylase comprises SEQ ID NO:12.

2. The engineered penicillin G acylase of claim 1 , wherein said penicillin G acylase is encoded by the polynucleotide sequence set forth in SEQ ID NO: 11.

Assignees

• Codexis Inc

Inventors

• Banerjee Goutami
• Yang Jie
• Zhang Xiyun
• Milczek Erika M
• Mayo Melissa Ann
• Jenne Stephan

Classifications

• A61P3/10

  for hyperglycaemia, e.g. antidiabetics · CPC title

• A61P5/50

  for increasing or potentiating the activity of insulin · CPC title

• C12N9/84 Primary

  Penicillin amidase {(3.5.1.11)} · CPC title

• C12P21/00

  Preparation of peptides or proteins (single cell protein C12N1/00) · CPC title

• C12Y305/01011

  Penicillin amidase (3.5.1.11), i.e. penicillin-amidohydrolase · CPC title