Methods for multi-dose synthesis of [F-18]FDDNP for clinical settings

Patent metadata

Field	Value
Publication number	US-10377701-B2
Application number	US-201715807306-A
Country	US
Kind code	B2
Filing date	Nov 8, 2017
Priority date	Nov 8, 2016
Publication date	Aug 13, 2019
Grant date	Aug 13, 2019

Abstract

A method of manufacturing 2-(1-{6-[(2-[F-18]fluoroethyl)(methyl)amino]-2-naphthyl}ethylidene)-malononitrile ([F-18]FDDNP) utilizes a semi-automated module that is used to perform fluorination, pre-purification, separation, product extraction, and formulation. The method is able to produce [F-18]FDDNP with high yields and ready for human administration under existing FDA regulations, and without the need for hazardous organic solvents such as dichloromethane (DCM), methanol (MeOH), and tetrahydrofuran (THF). The method also improves the speed with which [F-18]FDDNP can be synthesized with the method being able to generate a final product within about 90 to 100 minutes. This synthesis method is easily adaptable to FDA registered and approved automated synthesis systems.

First claim

What is claimed is:

1. A method of manufacturing 2-(1-{6-[(2-[F-18]fluoroethyl)(methyl)amino]-2-naphthyl}ethylidene)-malononitrile ([F-18]FDDNP) comprising:
trapping [F-18]fluoride ion in a resin cartridge;
eluting the [F-18]fluoride ion into a reaction vessel having a cryptand solution contained therein by passing a potassium salt solution followed by water through the resin cartridge, wherein a [F-18]fluoride/cryptand complex is formed therein;
subjecting the [F-18]fluoride/cryptand complex to multiple rounds of azeotropic evaporation with anhydrous acetonitrile to form dried [F-18]fluoride ion/cryptand complex residue in the reaction vessel;
reacting the dried [F-18]fluoride ion/cryptand complex with tosyloxy precursor 2-{[6-(2,2-dicyano-1-methylvinyl)-2-naphthyl](methyl)amino}ethyl-4-methylbenzenesulfonate (DDNPTs) in anhydrous acetonitrile to form a reaction product;
passing the reaction product through an alumina cartridge and into an injection vessel;
injecting the reaction product contained in the injection vessel to an HPLC column;
collecting a fraction containing [F-18]FDDNP from the HPLC column in a dilution vessel;
diluting the collected [F-18]FDDNP contained in the dilution vessel with water;
passing the diluted [F-18]FDDNP through a solid-phase extraction cartridge and eluting [F-18]FDDNP with ethanol; and
diluting the [F-18]FDDNP contained in ethanol with saline and human serum albumin to form a final product.

2. The method of claim 1 , further comprising transferring the final product into a sterile vial.

3. The method of claim 2 , wherein the final product is filtered with a filter during transfer to the sterile vial.

4. The method of claim 3 , further comprising rinsing final product residue through the filter with saline and human serum albumin into the sterile vial.

5. The method of claim 2 , wherein the [F-18]FDDNP in the sterile vial has a radiochemical yield of greater than 35%.

6. The method of claim 2 , wherein the [F-18]FDDNP in the sterile vial is produced in more than 400 mCi (corrected to EOB) amounts with a radiochemical yield of greater than 35% with one Ci of F-18 fluoride as starting cyclotron produced activity.

7. The method of claim 1 , wherein passing the reaction product directly through the alumina cartridge and into an injection vessel further comprises rinsing the reaction vessel with anhydrous acetonitrile and flowing the rinse through the alumina cartridge.

8. The method of claim 1 , further comprising adding chilled ammonium acetate (NH 4 OAc)/L-ascorbic acid to the injection vessel after passing the reaction product through an alumina cartridge and into an injection vessel.

9. The method of claim 1 , wherein the HPLC column is prepared with MeCN/ammonium acetate (NH 4 OAc) and L-ascorbic acid.

10. The method of claim 1 , wherein the final product is produced in less than 120 minutes.

11. The method of claim 1 , wherein the final product is produced in about 100 minutes or less.

12. The method of claim 1 , wherein the [F-18]FDDNP is produced using an automated synthesizer.

13. The method of claim 1 , wherein the [F-18]FDDNP is produced using at least some manual operations.

Assignees

• Univ California

Inventors

• Satyamurthy Nagichettiar
• Liu Jie
• Barrio Jorge R

Classifications

• C07C253/34

  Separation; Purification · CPC title

• C07C253/30 Primary

  by reactions not involving the formation of cyano groups · CPC title

• C07B2200/05

  Isotopically modified compounds, e.g. labelled · CPC title

• C07B59/001

  Acyclic or carbocyclic compounds · CPC title

• C07C255/34

  with cyano groups linked to the six-membered aromatic ring, or to the condensed ring system containing that ring, by unsaturated carbon chains · CPC title