Apparatus and method for generating droplets

Patent metadata

Field	Value
Publication number	US-10369536-B2
Application number	US-201715611399-A
Country	US
Kind code	B2
Filing date	Jun 1, 2017
Priority date	Dec 2, 2014
Publication date	Aug 6, 2019
Grant date	Aug 6, 2019

Abstract

An apparatus for generating one or several droplets of a first liquid in a second liquid immiscible with the first liquid includes a rotational body and a drive apparatus. The rotational body includes a fluid chamber, a fluid channel and a transition area. The transition area includes a first expansion area and a second expansion area. The drive apparatus is configured to provide the rotational body with such a rotation that the first liquid is supplied centrifugally to the fluid chamber and that centrifugally hydrodynamically induced pressure and lifting forces are caused due to the second expansion area, which cause a droplet break-off in the first liquid, such that a droplet of the first liquid embedded in the second liquid is generated.

First claim

The invention claimed is:

1. A method for generating at least one droplet of a first liquid in a second liquid immiscible with the first liquid by using an apparatus for generating at least one droplet of a first liquid in a second liquid immiscible with the first liquid, the apparatus comprising:
a rotational body comprising fluidic structures, the fluidic structures comprising: a fluid chamber configured to comprise the second liquid; a fluid channel leading to the fluid chamber and configured to cause a flow of the first liquid in a flow direction to the fluid chamber, and a transition area where the fluid channel opens to the fluid chamber, wherein the transition area comprises a first expansion area where the flow cross-section of the transition area for the flow of the first liquid expands in at least a first direction perpendicular to the flow direction and a second expansion area where the flow cross-section of the transition area for the flow of the first liquid expands in a second direction perpendicular to the flow direction and to the first direction, wherein the second expansion area is arranged downstream of the first expansion area with respect to the flow direction; and
a drive apparatus configured to provide the rotational body with a rotation, the method comprising:
inserting the first liquid in the fluid channel;
rotating the rotational body thereby supplying the first liquid centrifugally through the fluid channel to the fluid chamber in which the second liquid is arranged and inducing in the second expansion area centrifugally hydrodynamically induced forces acting on the first liquid thereby causing a droplet break-off of the first liquid and generation of a droplet of the first liquid in the second liquid,
wherein, after generating the droplet, the droplet is moved away from the transition area by the rotation due to different densities of the first liquid and the second liquid.

2. The method according to claim 1 , wherein the fluid channel opens to the fluid chamber in a radially outer area, wherein the second liquid comprises a higher density than the first liquid, wherein the method comprises maintaining the second liquid at the transition area by a centrifugal force acting on the second liquid.

3. The method according to claim 1 , wherein, in inserting the first fluid in the fluid channel, the first liquid is a biochemical reaction mixture, the method further comprising:
generating a plurality of droplets of the biochemical reaction mixture in the second liquid, and
exposing the rotational body to different temperatures after generating the plurality of droplets to perform a DNA verification reaction or RNA verification reaction of the biochemical reaction mixture and reading out the result of the DNA verification reaction or the RNA verification reaction via an optical system.

4. The method according to claim 1 , wherein in rotating the rotary body droplets are generated in parallel in the second liquid by supplying the first liquid centrifugally through a plurality of fluid channels, each of the fluid channels opening to the fluid chamber in a respective transition area.

5. The method according to claim 1 , wherein in rotating the rotary body droplets of different sizes are generated in parallel in the second liquid by supplying the first liquid centrifugally through a plurality of fluid channels, each of the fluid channels opening to the fluid chamber in which the second liquid is arranged in a respective transition area, wherein the transition areas of the plurality of fluid channels are dimensioned differently.

Assignees

• Hahn Schickard Ges Fuer Angewandte Forschung E V

Inventors

• Schwemmer Frank
• Schuler Friedrich

Classifications

• B01L3/502784

  specially adapted for droplet or plug flow, e.g. digital microfluidics · CPC title

• B01L2300/0803

  Disc shape · CPC title

• C12Q1/6806

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• B01L2300/0867

  Multiple inlets and one sample wells, e.g. mixing, dilution · CPC title

• B01L2200/0673

  Handling of plugs of fluid surrounded by immiscible fluid · CPC title