Method for producing an oil or fat component and method for producing higher unsaturated fatty acid using algae

Patent metadata

Field	Value
Publication number	US-10351882-B2
Application number	US-201414913618-A
Country	US
Kind code	B2
Filing date	Mar 17, 2014
Priority date	Aug 23, 2013
Publication date	Jul 16, 2019
Grant date	Jul 16, 2019

Abstract

There is provided a method for generating an oil/fat component, in which salt-tolerant algae are cultured in a culture medium of which the salt concentration has been increased in a stepwise manner.

First claim

What is claimed is:

1. A method for producing an oil or fat component, the method comprising:
culturing salt-tolerant algae belonging to Chlamydomonas in a multi-stage culture method with a total number of stages being 3 to 8, wherein the sea salt or sodium chloride concentration of a culture medium in a first stage is in the range of 0.5% by mass to 5% by mass of the total culture medium, and the sea salt or sodium chloride concentration to be increased for each stage after the first stage is in the range of 0.5% by mass to 5% by mass of the total culture medium;
recovering the salt-tolerant algae; and
harvesting the oil or fat component from the recovered salt-tolerant algae.

2. The method for producing an oil or fat component according to claim 1 , wherein the salt-tolerant algae are cultured in a culture medium of which the sea salt or sodium chloride concentration has been increased by 0.5% by mass to 2% by mass of the total culture medium per day in each stage of the multi-stage culture method.

3. The method for producing an oil or fat component according to claim 1 , further comprising:
performing cultivation under the condition in which the nitrogen content is 10 mg/L or less.

4. The method for producing an oil or fat component according to claim 1 , further comprising:
increasing the sea salt or sodium chloride concentration when a content of nitrate in the culture medium in the first stage becomes 10 mg/L or less in a case where the content thereof at a wavelength of 220 nm is measured.

5. The method for producing an oil or fat component according to claim 1 , wherein the culture medium contains seawater, concentrated seawater, or artificial seawater.

6. The method for producing an oil or fat component according to claim 1 , wherein the total number of stages being 4 to 6.

7. The method for producing an oil or fat component according to claim 1 , wherein the sea salt or sodium chloride concentration of the culture medium in the first stage is in the range of 0.5% by mass to 2% by mass of the total culture medium.

8. The method for producing an oil or fat component according to claim 1 , wherein a cultivation period in each stage is in the range of 1 day to 3 days.

9. The method for producing an oil or fat component according to claim 1 , wherein the salt-tolerant algae are cultured in a culture medium of which the sea salt or sodium chloride concentration has been increased by 0.5% by mass to 1.5% by mass of the total culture medium per day in each stage of the multi-stage culture method.

10. The method for producing an oil or fat component according to claim 1 , wherein the salt-tolerant algae are Chlamydomonas sp. JSC4 (FERM ABP-22266).

11. A method for producing a higher unsaturated fatty acid, the method comprising:
hydrolyzing an oil/fat component obtained by the method for producing an oil or fat component according to claim 10 .

12. A method for producing a higher unsaturated fatty acid, the method comprising:
hydrolyzing an oil or fat component obtained by the method according to claim 1 .

13. The method for producing a higher unsaturated fatty acid according to claim 12 , wherein the higher unsaturated fatty acid is oleic acid or linolenic acid.

Assignees

• Univ Kobe Nat Univ Corp
• Inter Univ Research Institute Corporation National Institutes Of Natural Sciences
• Dainippon Ink & Chemicals

Inventors

• Kondo Akihiko
• Hasunuma Tomohisa
• HO Shih-hsin
• Minagawa Jun
• Nishie Haruo
• TARODA Hiroyuki
• Chang Jo-Shu

Classifications

• C12P7/649

  Biodiesel, i.e. fatty acid alkyl esters · CPC title

• C12P7/6463 Primary

  obtained from glyceride producing microorganisms, e.g. single cell oil · CPC title

• C12P7/64

  Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats · CPC title

• C12P7/6409 Primary

  Fatty acids · CPC title