Method of producing fatty acids or lipids containing fatty acids using thioesterase variants
US-9222101-B2 · Dec 29, 2015 · US
US2016201098A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2016201098-A1 |
| Application number | US-201414913594-A |
| Country | US |
| Kind code | A1 |
| Filing date | Mar 17, 2014 |
| Priority date | Aug 23, 2013 |
| Publication date | Jul 14, 2016 |
| Grant date | — |
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There is provided a method for generating an oil/fat component by means of culturing algae, in which marine algae belonging to Chlamydomonas are cultured in a culture medium containing sea salt.
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1 . A method for generating an oil/fat component by means of culturing algae, the method comprising: culturing marine algae belonging to Chlamydomonas in a culture medium containing sea salt. 2 . The method for generating an oil/fat component according to claim 1 , wherein the algae belonging to Chlamydomonas are Chlamydomonas sp. JSC4. 3 . The method for generating an oil/fat component according to claim 1 , wherein, the amount of nitrate in the culture containing sea salt measured using a wavelength of 220 nm is 10 mg/L or less. 4 . The method for generating an oil/fat component according to claim 1 , wherein the mass % of the sea salt in the culture medium is in the range of 0.5% by mass to 5% by mass. 5 . The method for generating an oil/fat component according to claim 1 , wherein the culture medium containing sea salt contains one of the group consisting of seawater, concentrated seawater, and artificial seawater. 6 . A method for producing a higher unsaturated fatty acid, the method comprising: hydrolyzing an oil/fat component obtained by the method for generating an oil/fat component according to claim 1 . 7 . The method for producing a higher unsaturated fatty acid according to claim 6 , wherein the higher unsaturated fatty acid is oleic acid or linolenic acid. 8 . Chlamydomonas sp. JSC4 which has an oil/fat component producing ability. 9 . The method for generating an oil/fat component according to claim 2 , wherein, the amount of nitrate in the culture containing sea salt measured using a wavelength of 220 nm is 10 or less. 10 . The method for generating an oil/fat component according to claim 2 , wherein the mass % of the sea salt in the culture medium is in the range of 0.5% by mass to 5% by mass. 11 . The method for generating an oil/fat component according to claim 3 , wherein the mass % of the sea salt in the culture medium is in the range of 0.5% by mass to 5% by mass. 12 . The method for generating an oil/fat component according to claim 9 , wherein the mass % of the sea salt in the culture medium is in the range of 0.5% by mass to 5% by mass. 13 . The method for generating an oil/fat component according to claim 2 , wherein the culture medium containing sea salt contains one of the group consisting of seawater, concentrated seawater, and artificial seawater. 14 . The method for generating an oil/fat component according to claim 3 , wherein the culture medium containing sea salt contains one of the group consisting of seawater, concentrated seawater, and artificial seawater. 15 . The method for generating an oil/fat component according to claim 4 , wherein the culture medium containing sea salt contains one of the group consisting of seawater, concentrated seawater, and artificial seawater. 16 . The method for generating an oil/fat component according to claim 9 , wherein the culture medium containing sea salt contains one of the group consisting of seawater, concentrated seawater, and artificial seawater. 17 . A method for producing a higher unsaturated fatty acid, the method comprising: hydrolyzing an oil/fat component obtained by the method for generating an oil/fat component according to claim 2 . 18 . A method for producing a higher unsaturated fatty acid, the method comprising: hydrolyzing an oil/fat component obtained by the method for generating an oil/fat component according to claim 3 . 19 . A method for producing a higher unsaturated fatty acid, the method comprising: hydrolyzing an oil/fat component obtained by the method for generating an oil/fat component according to claim 4 . 20 . A method for producing a higher unsaturated fatty acid, the method comprising: hydrolyzing an oil/fat component obtained by the method for generating an oil/fat component according to claim 5 .
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