Adsorptive membranes for trapping viruses

Patent metadata

Field	Value
Publication number	US-10316296-B2
Application number	US-201715820766-A
Country	US
Kind code	B2
Filing date	Nov 22, 2017
Priority date	Jul 14, 2006
Publication date	Jun 11, 2019
Grant date	Jun 11, 2019

Abstract

A disposable, virus-trapping membrane, and a corresponding method to remove viruses from solution are described. The membrane includes a disposable, micro-porous filter membrane and a ligand immobilized on the membrane. The ligand irreversibly and selectively binds viruses. The ligand also has a pKa sufficiently high to repel antibodies via electrostatic charge repulsion.

First claim

What is claimed is:

1. A disposable, virus-trapping membrane comprising:
a disposable, micro-porous filter membrane; and
a multi-modal anion-exchange ligand that has a pKa sufficiently high to repel basic proteins via electrostatic charge repulsion immobilized on the filter membrane, wherein the ligand comprises one or more of tyrosinol, tryptophanol, octopamine, 1,3-diamino-2-hydroxypropane, tris(2-aminoethyl)amine, and agmatine,
wherein the virus-trapping membrane:
(a) binds neutral viruses; and
(b) yields a log-reduction value (LRV) of at least 1.0 for the neutral viruses disposed in a solution comprising 50 mM salt.

2. The virus-trapping membrane of claim 1 , wherein the ligand comprises one or more of tryptophanol, tris(2-aminoethyl)amine, and agmatine, and wherein the virus-trapping membrane is dimensioned and configured to yield a log-reduction value (LRV) of at least 1.0 for neutral viruses disposed in a solution comprising 150 mM salt.

3. The virus-trapping membrane of claim 1 , wherein the ligand comprises one or more of tris(2-aminoethyl)amine and agmatine, and wherein the virus-trapping membrane is dimensioned and configured to yield a log-reduction value (LRV) of at least 5.0 for neutral viruses disposed in a solution comprising 50 mM salt.

4. The virus-trapping membrane of claim 1 , wherein the ligand comprises one or more of tris(2-aminoethyl)amine and agmatine, and wherein the virus-trapping membrane is dimensioned and configured to yield a log-reduction value (LRV) of at least 5.0 for neutral viruses disposed in a solution comprising 150 mM salt.

5. The virus-trapping membrane of claim 1 , wherein the filter membrane comprises a polymer substrate selected from the group consisting of polyvinylidene difluoride, polytetrafluorethylene, polyamides, polyamide-imides, polysulfones, polyethersulfones, and polyphenylsulfones.

6. The virus-trapping membrane of claim 1 , wherein the ligand comprises agmatine.

7. The virus-trapping membrane of claim 6 , wherein the virus-trapping membrane is dimensioned and configured to yield a log-reduction value (LRV) of at least 1.0 for neutral viruses disposed in a solution comprising 150 mM salt.

8. The virus-trapping membrane of claim 6 , wherein the virus-trapping membrane is dimensioned and configured to yield a log-reduction value (LRV) of at least 5.0 for neutral viruses disposed in a solution comprising 50 mM salt.

9. The virus-trapping membrane of claim 6 , wherein the virus-trapping membrane is dimensioned and configured to yield a log-reduction value (LRV) of at least 5.0 for neutral viruses disposed in a solution comprising 150 mM salt.

10. The virus-trapping membrane of claim 6 , wherein the filter membrane comprises a polymer substrate selected from the group consisting of polyvinylidene difluoride, polytetrafluorethylene, polyamides, polyamide-imides, polysulfones, polyethersulfones, and polyphenylsulfones.

11. The virus-trapping membrane of claim 1 , wherein the ligand comprises tris(2-aminoethyl)amine.

12. The virus-trapping membrane of claim 11 , wherein the virus-trapping membrane is dimensioned and configured to yield a log-reduction value (LRV) of at least 1.0 for neutral viruses disposed in a solution comprising 150 mM salt.

13. The virus-trapping membrane of claim 11 , wherein the virus-trapping membrane is dimensioned and configured to yield a log-reduction value (LRV) of at least 5.0 for neutral viruses disposed in a solution comprising 50 mM salt.

14. The virus-trapping membrane of claim 11 , wherein the virus-trapping membrane is dimensioned and configured to yield a log-reduction value (LRV) of at least 5.0 for neutral viruses disposed in a solution comprising 150 mM salt.

15. The virus-trapping membrane of claim 11 , wherein the filter membrane comprises a polymer substrate selected from the group consisting of polyvinylidene difluoride, polytetrafluorethylene, polyamides, polyamide-imides, polysulfones, polyethersulfones, and polyphenylsulfones.

16. The method of claim 1 , wherein the filter membrane has a membrane pore size of from 0.1 μm to 10 μm.

17. The virus-trapping membrane of claim 16 , wherein the ligand comprises one or more of tryptophanol, tris(2-aminoethyl)amine, and agmatine, and wherein the virus-trapping membrane is dimensioned and configured to yield a log-reduction value (LRV) of at least 1.0 for neutral viruses disposed in a solution comprising 150 mM salt.

18. The virus-trapping membrane of claim 16 , wherein the ligand comprises one or more of tris(2-aminoethyl)amine and agmatine, and wherein the virus-trapping membrane is dimensioned and configured to yield a log-reduction value (LRV) of at least 5.0 for neutral viruses disposed in a solution comprising 50 mM salt.

19. The virus-trapping membrane of claim 16 , wherein the ligand comprises one or more of tris(2-aminoethyl)amine and agmatine, and wherein the virus-trapping membrane is dimensioned and configured to yield a log-reduction value (LRV) of at least 5.0 for neutral viruses disposed in a solution comprising 150 mM salt.

20. The virus-trapping membrane of claim 16 , wherein the filter membrane comprises a polymer substrate selected from the group consisting of polyvinylidene difluoride, polytetrafluorethylene, polyamides, polyamide-imides, polysulfones, polyethersulfones, and polyphenylsulfones.

21. The virus-trapping membrane of claim 16 , wherein the ligand comprises agmatine.

22. The virus-trapping membrane of claim 16 , wherein the ligand comprises tris(2-aminoethyl)amine.

23. A method of removing viruses from a solution suspected of containing viruses, the method comprising contacting a solution suspected of containing viruses with the virus-trapping membrane of claim 1 .

Assignees

• Wisconsin Alumni Res Found

Inventors

• Etzel Mark R

Classifications

• B01J20/321

  consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds · CPC title

• B01J20/2808

  being less than 2 nm, i.e. micropores or nanopores · CPC title

• B01J20/3219

  involving a particular spacer or linking group, e.g. for attaching an active group · CPC title

• B01D2325/12

  Adsorbents being present on the surface of the membranes or in the pores · CPC title

• B01J20/3285

  Coating or impregnation layers comprising different type of functional groups or interactions, e.g. different ligands in various parts of the sorbent, mixed mode, dual zone, bimodal, multimodal, ionic or hydrophobic, cationic or anionic, hydrophilic or hydrophobic · CPC title