Adsorptive membranes for trapping viruses

Patent metadata

Field	Value
Publication number	US-9856459-B2
Application number	US-201615163050-A
Country	US
Kind code	B2
Filing date	May 24, 2016
Priority date	Jul 14, 2006
Publication date	Jan 2, 2018
Grant date	Jan 2, 2018

Abstract

A disposable, virus-trapping membrane, and a corresponding method to remove viruses from solution are described. The membrane includes a disposable, micro-porous filter membrane and a ligand immobilized on the membrane. The ligand irreversibly and selectively binds viruses. The ligand also has a pKa sufficiently high to repel antibodies via electrostatic charge repulsion.

First claim

What is claimed is:

1. A method of removing viruses from a solution suspected of containing viruses, the method comprising:
contacting a solution suspected of containing viruses with a virus-trapping membrane, wherein the solution comprises a monoclonal antibody, wherein the virus-trapping membrane comprises a disposable, micro-porous filter membrane and a ligand immobilized on the membrane, wherein the ligand is tyrosinol.

2. The method of claim 1 , wherein the filter membrane comprises a polymer substrate selected from the group consisting of polyvinylidene difluoride, polytetrafluorethylene, polyamides, polyamide-imides, polysulfones, polyethersulfones, and polyphenylsulfones.

3. The method of claim 2 , wherein the filter membrane has a membrane pore size of from 0.1 μm to 10 μm.

4. The method of claim 3 , wherein the solution comprises from 50 mM to 150 mM salt.

5. The method of claim 2 , wherein the solution comprises from 50 mM to 150 mM salt.

6. The method of claim 1 , wherein the filter membrane has a membrane pore size of from 0.1 μm to 10 μm.

7. The method of claim 6 , wherein the solution comprises from 50 mM to 150 mM salt.

8. The method of claim 1 , wherein the solution comprises from 50 mM to 150 mM salt.

9. The method of claim 1 , wherein the filter membrane has a membrane pore size of from 0.5 μm to 1.5 μm.

10. The method of claim 9 , wherein the filter membrane comprises a polymer substrate selected from the group consisting of polyvinylidene difluoride, polytetrafluorethylene, polyamides, polyamide-imides, polysulfones, polyethersulfones, and polyphenylsulfones.

11. The method of claim 10 , wherein the solution comprises from 50 mM to 150 mM salt.

12. The method of claim 9 , wherein the solution comprises from 50 mM to 150 mM salt.

Assignees

• Wisconsin Alumni Res Found

Inventors

• Etzel Mark R

Classifications

• B01J20/3251

  comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulphur · CPC title

• B01J20/3253

  comprising a cyclic structure not containing any of the heteroatoms nitrogen, oxygen or sulfur, e.g. aromatic structures · CPC title

• B01J20/2808

  being less than 2 nm, i.e. micropores or nanopores · CPC title

• B01D2325/12

  Adsorbents being present on the surface of the membranes or in the pores · CPC title

• B01J20/262

  obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation (macromolecular compounds obtained otherwise than by reactions only involving unsaturated carbon-to-carbon bonds per se C08G) · CPC title