Implantable biocompatible reactor

Patent metadata

Field	Value
Publication number	US-10316284-B2
Application number	US-201515126236-A
Country	US
Kind code	B2
Filing date	Mar 23, 2015
Priority date	Mar 25, 2014
Publication date	Jun 11, 2019
Grant date	Jun 11, 2019

Abstract

The invention concerns a bioreactor obtained by compressing a mixture of an enzyme, a conductor and chitosan. The conductor can consist of carbon nanotubes. This bioreactor can be produced according to the following steps: preparing a mixture of powders in which the proportion of enzyme powder relative to a carbon nanotube powder is of the order of 50/50 by weight; preparing a viscous solution of chitosan in a ratio of 5 to 15 (in mg) of chitosan to 0.75 to 1.25 (in ml) of acetic acid diluted to 0.4 to 0.6% by volume in water; adding the viscous chitosan to the mixture of powders in a proportion be weight of 3 to 5 of powder to 5 to 10 of chitosan; carrying out a first compression followed by light grinding; carrying out a second compression to produce a pellet; and drying at ambient temperature.

First claim

The invention claimed is:

1. A bioreactor obtained by a method comprising the steps of:
preparing a mixture of an enzyme powder and a carbon nanotube powder where the proportion of enzyme powder relative to carbon nanotube powder is of the order of 50/50 by weight;
preparing a viscous solution of chitosan in a ratio of 5 to 15 (in mg) of chitosan to 0.75 to 1.25 (in ml) of acetic acid diluted to 0.4 to 0.6% by volume in water;
adding the viscous chitosan to the mixture of enzyme powder and carbon nanotube powder in a proportion by weight of 3 to 5 of this mixture to 5 to 10 of chitosan;
carrying out a first compression followed by light grinding;
carrying out a second compression to produce a pellet; and
drying at ambient temperature.

2. The bioreactor of claim 1 , wherein the pressure applied during the first and the second compression is within a range from 2,000 to 6,000 kPa.

3. The bioreactor of claim 1 , wherein the solution comprises from 0.002 to 0.005% in mass per volume of genipin.

4. The bioreactor of claim 1 , wherein the solution comprises from 0.001 to 0.005% in mass per volume of caffeic acid.

5. The bioreactor of claim 1 , wherein a porous chitosan-based membrane is laid on an active surface of the bioreactor and bonded at the periphery thereof.

6. The bioreactor of claim 5 , wherein the porous chitosan-based membrane is obtained by a method comprising the steps of:
preparing a solution in a ratio of 5 to 15 (in mg) of chitosan to 0.75 to 1.25 (in ml) of acetic acid diluted to 0.4 to 0.6% in water;
stirring;
pouring on a smooth support; and
drying for a period from 2 to 4 days at ambient temperature.

7. The bioreactor of claim 5 , having a pellet-shaped bioelectrode, wherein a conductor is glued by means of conductive glue to the surface of the pellet-shaped bioelectrode opposite to the active surface.

8. The bioreactor of claim 5 , wherein the membrane comprises a plurality of pores having an average diameter in the range from 1 to 10 nanometers.

9. The bioreactor of claim 5 , wherein the membrane comprises a smooth surface facing the pellet-shaped electrode and a rough surface facing outwards.

10. The bioreactor of claim 7 , wherein the membrane comprises a plurality of pores having an average diameter in the range from 1 to 10 nanometers.

11. The bioreactor of claim 7 , wherein the membrane comprises a smooth surface facing the pellet-shaped electrode and a rough surface facing outwards.

12. The bioreactor of claim 2 , wherein the solution comprises from 0.002 to 0.005% in mass per volume of genipin.

13. The bioreactor of claim 2 , wherein the solution comprises from 0.001 to 0.005% in mass per volume of caffeic acid.

Assignees

• Univ Grenoble Alpes

Inventors

• El Ichi Sarra
• Martin Donald K
• Cinquin Philippe
• Zebda Abdelkader

Classifications

• H01M4/8896

  Pressing, rolling, calendering (membrane electrode assemblies H01M8/1004) · CPC title

• H01M2250/30

  Fuel cells in portable systems, e.g. mobile phone, laptop · CPC title

• G01N27/3271

  Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood (amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001) · CPC title

• B29L2031/34

  Electrical apparatus, e.g. sparking plugs or parts thereof · CPC title

• H01M4/9008

  Organic or organo-metallic compounds · CPC title