Electrospun nanofibrous membranes and disposable glucose biosensor

Patent metadata

Field	Value
Publication number	US-9708640-B2
Application number	US-201514791872-A
Country	US
Kind code	B2
Filing date	Jul 6, 2015
Priority date	Dec 1, 2014
Publication date	Jul 18, 2017
Grant date	Jul 18, 2017

Abstract

An electrospun nanofibrous membrane is sheet like and is formed by multiple glucose oxidase/potassium hexacyanoferrate(III) modified electrospun nanofibers. The glucose oxidase/potassium hexacyanoferrate(III) modified electrospun nanofibers are PVA electrospun nanofibers containing glucose oxidase and potassium hexacyanoferrate(III) homogeneously dispersed therein. The glucose oxidase/potassium hexacyanoferrate(III) modified electrospun nanofibers are PVA electrospun nanofibers and are cross-linked by glutaraldehyde vapor with ultrasonic energy assistance. Graphene modified PVA/GOx electrospun membranes were prepared to examine the immobilization mechanism between graphene and GOx. The electrochemical measurement results show that the sensitivities increased with increasing graphene concentrations up to 20 ppm. The highest sensitivity recorded 38.7 μA/mM was for a PVA/GOx membrane with 20 ppm graphene representing a 109% increase over a membrane made without graphene.

First claim

What is claimed is:

1. A disposable glucose biosensor comprising:
a sensor body and an electrode set mounted on the sensor body; and
an electrospun nanofibrous membrane attached on the electrode set, wherein the electrospun nanofibrous membrane is formed by multiple electrospun nanofibers, wherein each nanofiber is a polyvinyl alcohol (PVA) electrospun nanofiber containing glucose oxidase and potassium hexacyanoferrate(III) homogeneously dispersed therein, wherein the electrospun nanofibrous membrane is cross-linked by glutaraldehyde vapor or heat treatment, wherein the PVA electrospun nanofiber containing glucose oxidase and potassium hexacyanoferrate(III) homogeneously dispersed therein comprises a conductive material, wherein the conductive material is nano-gold attached to the electrospun nanofibrous membrane; and wherein a solid content of the nano-gold in the PVA electrospun nanofiber containing glucose oxidase and potassium hexacyanoferrate(III) homogeneously dispersed therein is in a range of 10˜2000 ppm.

2. The disposable glucose biosensor as claimed in claim 1 , wherein:
a solid content of the PVA in the electrospun nanofibrous membrane is in a range of 90%˜96%;
a solid content of the glucose oxidase in the electrospun nanofibrous membrane is in a range of 1˜3%; and
a solid content of the potassium hexacyanoferrate(III) in the electrospun nanofibrous membrane is in a range of 2˜8%.

3. An electrospun nanofibrous membrane comprising:
multiple electrospun nanofibers, wherein:
the multiple electrospun nanofibers are electrospun by a matrix material solution and containing glucose oxidase and potassium hexacyanoferrate(III) homogeneously dispersed therein; and
the electrospun nanofibers are cross-linked by glutaraldehyde vapor or heat treatment; and

a conductive material, wherein:
the conductive material is nano-gold which is attached to or mounted in the multiple electrospun nanofibers; and

a solid content of the nano-gold in the multiple electrospun nanofibers is in a range of 10˜2000 ppm.

4. The electrospun nanofibrous membrane as claimed in claim 3 , wherein the matrix material solution is a polyvinyl alcohol (PVA) solution and the multiple electrospun nanofibers are PVA nanofibers, and wherein the PVA nanofibers are cross-linked by the glutaraldehyde vapor with ultrasonic energy assistance.

5. The electrospun nanofibrous membrane as claimed in claim 4 , wherein:
a solid content of the PVA in the electrospun nanofibrous membrane is 90%˜96%;
a solid content of the glucose oxidase in the electrospun nanofibrous membrane is 1˜3%; and
a solid content of the potassium hexacyanoferrate(III) in the electrospun nanofibrous membrane is 2˜8%.

6. The electrospun nanofibrous membrane as claimed in claim 5 , wherein the nano-gold is attached to the multiple electrospun nanofibers.

Assignees

• Univ Nat Taiwan Science Tech

Inventors

• Wu Chang-Mou
• Yu Hsiu-An
• Huang Chun-Chung

Classifications

• C12Q1/54 Primary

  involving glucose or galactose · CPC title

• G01N27/3278

  involving nanosized elements, e.g. nanogaps or nanoparticles (nanopores G01N33/48721; magnetic beads G01N27/745) · CPC title

• B82Y15/00

  Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors · CPC title

• C12Q1/006

  for glucose · CPC title

• C12Y101/03004

  Glucose oxidase (1.1.3.4) · CPC title