Genetic loci associated with Phytophthora tolerance in soybean and methods of use

Patent metadata

Field	Value
Publication number	US-9493843-B2
Application number	US-201313782013-A
Country	US
Kind code	B2
Filing date	Mar 1, 2013
Priority date	Dec 20, 2012
Publication date	Nov 15, 2016
Grant date	Nov 15, 2016

Abstract

Various methods and compositions are provided for identifying and/or selecting soybean plants or soybean germplasm with tolerance or improved tolerance to Phytophthora infection. In certain embodiments, the method comprises detecting at least one marker locus that is associated with tolerance to Phytophthora infection. In other embodiments, the method further comprises detecting at least one marker profile or haplotype associated with tolerance to Phytophthora infection. In further embodiments, the method comprises crossing a selected soybean plant with a second soybean plant. Further provided are markers, primers, probes and kits useful for identifying and/or selecting soybean plants or soybean germplasm with tolerance or improved tolerance to Phytophthora infection.

First claim

That which is claimed:

1. A method of introgressing tolerance to Phytophthora infection, from a first soybean plant or a first soybean germplasm that displays tolerance to Phytophthora infection compared to a susceptible plant, into a second soybean plant or second soybean germplasm, the method comprising:
detecting in the genome of said first soybean plant or in the genome of said first soybean germplasm at least one marker locus that is associated with the tolerance, wherein the at least one marker locus comprises 808291-1,
wherein the detecting comprises amplifying the at least one marker locus and detecting the resulting amplified marker amplicon,

thereby selecting a first soybean plant or a first soybean germplasm that displays tolerance to Phytophthora infection compared to a susceptible plant, and
crossing the selected first soybean plant or first soybean germplasm comprising said at least one maker locus with a second soybean plant or second soybean germplasm,
thereby introgressing the tolerance to Phytophthora infection into said second soybean plant or second soybean germplasm.

2. The method of claim 1 , wherein at least two marker loci are detected.

3. The method of claim 2 , wherein the at least two marker loci comprise a haplotype or a marker profile that is associated with said tolerance.

4. The method of claim 1 , wherein the germplasm comprises a soybean variety.

5. The method of claim 1 , wherein the second soybean plant or second soybean germplasm comprises an exotic soybean strain or an elite soybean strain.

6. The method of claim 1 , further comprising DNA sequencing of at least one of said marker loci.

7. The method of claim 1 , wherein the amplifying comprises:
a) admixing an amplification primer or amplification primer pair for each marker locus being amplified with a nucleic acid isolated from the first soybean plant or the first soybean germplasm, wherein the primer or primer pair is complementary or partially complementary to the genomic locus comprising the marker locus, and is capable of initiating DNA polymerization by a DNA polymerase using the soybean nucleic acid as a template; and
b) extending the primer or primer pair in a DNA polymerization reaction comprising a DNA polymerase and a template nucleic acid to generate at least one amplicon.

8. The method of claim 7 , wherein said method comprises
amplifying one or more polynucleotides comprising SEQ ID NOs: 155 or 156.

9. The method of claim 7 , wherein said primer or primer pair comprises
a fragment of one or more polynucleotides comprising SEQ ID NOs: 155 or 156.

10. The method of claim 9 , wherein said primer or primer pair comprises a nucleic acid sequence comprising a nucleic acid sequence comprising SEQ ID NOs: 1 or 2.

11. The method of claim 10 , wherein said primer pair comprises: SEQ ID NO: 1 and SEQ ID NO:2.

12. The method of claim 7 , wherein the method further comprises providing one or more labeled nucleic acid probes suitable for detection of each marker locus being amplified.

13. The method of claim 12 , wherein said labeled nucleic acid probe comprises a nucleic acid sequence comprising:
one or more polynucleotides comprising SEQ ID NOs: 155 or 156.

Assignees

• Pioneer Hi Bred Int

Inventors

• Chaky Julian M
• Jessen Holly J
• Shendelman Joshua M
• Stephens Paul A
• Webb David M
• Woodward John B
• Yang Meizhu

Classifications

• C12Q2600/172

  Haplotypes · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C12Q1/6895 Primary

  for plants, fungi or algae · CPC title

• A01H4/00

  Plant reproduction by tissue culture techniques {; Tissue culture techniques therefor} · CPC title