Bioreactor arrangement, shaking device and method for irradiating a medium in a bioreactor

Patent metadata

Field	Value
Publication number	US-10253290-B2
Application number	US-201113635769-A
Country	US
Kind code	B2
Filing date	Apr 21, 2011
Priority date	Apr 28, 2010
Publication date	Apr 9, 2019
Grant date	Apr 9, 2019

Abstract

A bioreactor arrangement has a bioreactor and an illuminating body for illuminating a medium in a reactor interior. The illuminating body has at least one emission surface via which light, reflected by at least one end surface into the reactor interior, is emitted. The bioreactor is formed as a container having a transparent wall. The illuminating body is outside the reactor interior, next to at least one subregion of the transparent wall. The illuminating body forms a support surface for the bioreactor with its emission surface. A shaking device has a reactor holder that can be in oscillated, for a bioreactor with a transparent wall. The reactor holder has a two-dimensional illuminating body, the emission surface of which forms a support surface for the bioreactor, via which light can be reflected into the reactor interior of the bioreactor.

First claim

The invention claimed is:

1. A bioreactor arrangement ( 1 ), comprising a bioreactor container ( 2 ) with a reactor interior ( 6 ) for containing a medium ( 20 ) arranged in the reactor interior ( 6 ), the bioreactor container ( 2 ) having a transparent wall ( 5 ), an illuminating body ( 3 ) arranged outside the reactor interior ( 6 ) and having an emission surface ( 7 ) next to the transparent wall ( 5 ), the emission surface ( 7 ) of the illuminating body ( 3 ) forming a bearing surface ( 8 ) that supports the bioreactor container ( 2 ) from below and at least one end surface ( 15 , 16 ) intersecting the bearing surface ( 8 ); and at least one light source ( 17 , 18 ) arranged to project light into the at least one end surface ( 15 , 16 ) of the illuminating body ( 3 ) and directing the light into the illuminating body ( 3 ), the illuminating body ( 3 ) being configured so that the light projected into the at least one end surface ( 15 , 16 ) is redirected within the illuminating body ( 3 ) and emitted from the emission surface ( 7 ), through the transparent wall ( 5 ) and into the reactor interior ( 6 ) of the reactor container ( 2 ) supported on the emission surface ( 7 ).

2. The bioreactor arrangement of claim 1 , wherein the illuminating body ( 3 ) is arranged on a reactor holder ( 9 ) of a shaking device ( 4 ).

3. The bioreactor arrangement of claim 1 , wherein the bioreactor ( 2 ) is a flexible plastic bag for single use.

4. The bioreactor arrangement of claim 1 , wherein the illuminating body ( 3 ) is a flat plate, and that the at least one light source ( 17 , 18 ) includes a lighting strip ( 17 ) comprising a multiplicity of LED light sources ( 18 ) arranged on at least one end surface ( 15 , 16 ).

5. The bioreactor arrangement of claim 1 , wherein the illuminating body ( 3 ) comprises light-scattering particles ( 19 ) inside.

6. The bioreactor arrangement of claim 1 , wherein the emission surface ( 7 ) facing toward the bioreactor ( 2 ) comprises a light-scattering structure.

7. The bioreactor arrangement of claim 1 , wherein the illuminating body ( 3 ) is formed from an acrylic glass.

8. A bioreactor arrangement ( 1 ), comprising: a flexible bioreactor container ( 2 ) with a reactor interior ( 6 ) for containing a medium ( 20 ), the bioreactor container ( 2 ) having a transparent wall ( 5 ); an illuminating body ( 3 ) arranged outside the reactor interior ( 6 ) and defining a movable holder of a shaking device ( 4 ), the illuminating body ( 3 ) having an emission surface ( 7 ) next to the transparent wall ( 5 ) and at least one end surface ( 15 , 16 ) intersecting the bearing surface ( 8 ), the emission surface ( 7 ) of the illuminating body ( 3 ) forming a bearing surface ( 8 ) that supports the bioreactor container ( 2 ) from below; and a lighting strip ( 17 ) comprising a multiplicity of LED light sources ( 18 ) arranged on the at least one end surface ( 15 , 16 ) of the illuminating body ( 3 ), the LED light sources ( 18 ) directing light into the at least one end surface ( 15 , 16 ) of the illuminating body ( 3 ), wherein the illuminating body ( 3 ) is configured so that the light entering the at least one end surface ( 15 , 16 ) is redirected within the illuminating body ( 3 ) from the at least one end surface ( 15 , 16 ) to the emission surface ( 7 ), through the transparent wall ( 5 ) and into the reactor interior ( 6 ) of the flexible reactor container ( 2 ) that is supported on the emission surface ( 7 ).

9. The bioreactor arrangement ( 1 ) of claim 8 , wherein the flexible bioreactor container ( 2 ) has no light sources in the reactor interior ( 6 ).

10. The bioreactor arrangement of claim 9 , wherein the illuminating body ( 3 ) comprises light-scattering particles ( 19 ) inside.

11. The bioreactor arrangement of claim 9 , wherein the emission surface ( 7 ) facing toward the bioreactor ( 2 ) comprises a light-scattering structure.

Assignees

• Roell Marcel
• Boettcher Lars
• Sartorius Stedim Biotech Gmbh

Inventors

• Roell Marcel
• Boettcher Lars

Classifications

• C12M31/02 Primary

  located outside the reactor · CPC title

• C12M27/16

  Vibrating; Shaking; Tilting · CPC title

• C12M21/02

  Photobioreactors (culturing algae A01G33/00, A01H4/001, C12N1/12) · CPC title

• C12M23/04

  Flat or tray type, drawers (C12M23/10, C12M23/12, C12M23/16 take precedence) · CPC title

• C12M23/26

  flexible (flexible containers for laboratory use B01L3/505) · CPC title