Microfluidic photoporation

Patent metadata

Field	Value
Publication number	US-9683209-B2
Application number	US-201214110384-A
Country	US
Kind code	B2
Filing date	Jun 20, 2012
Priority date	Jun 21, 2011
Publication date	Jun 20, 2017
Grant date	Jun 20, 2017

Abstract

A cell permeabilizing microfluidic system for permeabilizing one or more cells in a fluid flow. The system has a microfluidic channel for channeling at least one cell in a fluid flow and an optical source for generating a beam of light for permeabilizing the at least one cell, wherein the channel and the source are arranged so that the light beam and fluid flow are collinear in a permeabilization part of the channel and cells are permeabilized within the permeabilization part.

First claim

The invention claimed is:

1. A cell permeabilizing microfluidic system for permeabilizing one or more cells in a fluid flow, the system comprising:
a microfluidic channel for channeling at least one cell in a fluid flow; and
an optical source for generating a beam of light for permeabilizing the at least one cell,
wherein the microfluidic channel and the optical source are arranged so that the light beam and fluid flow are collinear in a permeabilization part of the microfluidic channel and cells are permeabilized within the permeabilization part, and the light beam is non-diffracting and so propagation invariant in the permeabilization part of the microfluidic channel.

2. The microfluidic system as claimed in claim 1 comprising cell guiding means for guiding the cells in a confinement region contained within the fluid flow in the permeabilization part.

3. The microfluidic system as claimed in claim 2 , wherein the cell guiding means are based on hydrodynamic focusing.

4. The microfluidic system as claimed in claim 3 , wherein the cell guiding means comprises a three-dimensional nozzle.

5. The microfluidic system as claimed in claim 2 , wherein the light beam extends over a volume that includes the confinement region.

6. The microfluidic system as claimed in claim 1 , wherein the microfluidic channel has a bend and the light beam is coupled into the microfluidic channel at the bend.

7. The microfluidic system as claimed in claim 1 , wherein the microfluidic channel is L-shaped or S-shaped or U-shaped.

8. The microfluidic system as claimed in claim 1 , wherein the microfluidic channel and the optical source are arranged so that the light beam is parallel to the microfluidic channel walls.

9. The microfluidic system as claimed in claim 1 , wherein the microfluidic channel and the optical source are arranged so that, in the permeabilization part, the cell flow is moving in a direction opposite to the direction of propagation of the light beam.

10. The microfluidic system as claimed in claim 1 further comprising means of varying the light beam intensity profile.

11. The microfluidic system as claimed in claim 10 , wherein the means of varying the light intensity profile include an axicon lens.

12. The microfluidic system as claimed in claim 10 , wherein the means of varying the light intensity profile include a spatial light modulator.

13. The microfluidic system as claimed in claim 1 , wherein the non-diffractive light beam is a Bessel beam.

14. The microfluidic system as claimed in claim 13 , wherein the Bessel beam is propagation invariant along the length of the permeabilization part of the microfluidic channel.

15. The microfluidic system as claimed in claim 1 , wherein one or more inlets are provided for introducing one or more additional fluids into the microfluidic channel.

16. The microfluidic system as claimed in claim 15 , wherein the one or more inlets are positioned to allow one or more additional fluids to be introduced into the microfluidic channel before the permeabilization part of the microfluidic channel.

17. The microfluidic system as claimed in claim 1 , wherein light is coupled into the permeabilization part of the microfluidic channel using an optical fiber and/or a waveguide.

18. The microfluidic system as claimed in claim 1 , wherein the microfluidic channel has a square cross-section or a circular cross-section or a rectangular cross-section.

19. The microfluidic system as claimed in claim 1 , wherein the microfluidic channel has cross sectional dimensions in the range of 1-500 μm.

20. The microfluidic system as claimed in claim 1 , wherein the microfluidic channel is formed on-chip.

21. The microfluidic system as claimed in claim 1 , wherein the microfluidic channel and optical source are on-chip forming an integrated on chip device.

22. The microfluidic system as claimed in claim 1 , wherein the microfluidic system is arranged so that cell function is preserved after permeabilization.

23. A method for permeabilizing one or more cells in a fluid flow in a microfluidic channel comprising exposing cells in a permeabilization part of the microfluidic channel to a light beam that is collinear with the direction of fluid flow in the permeabilization part of the microfluidic channel to thereby permeabilize the cells, wherein the light beam is non-diffracting and so propagation invariant in the permeabilization part of the microfluidic channel.

24. The method as claimed in claim 23 , wherein the light beam is a Bessel beam.

25. The method as claimed in claim 23 further comprising introducing one or more additional fluids into the fluid flow prior to light exposure.

Assignees

• Dholakia Kishan
• Marchington Robert
• Arita Yoshihiko
• Gunn-Moore Frank
• Univ Court Univ St Andrews

Inventors

• Dholakia Kishan
• Marchington Robert
• Arita Yoshihiko
• Gunn-Moore Frank

Classifications

• B01L3/502761

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• G01N21/05

  Flow-through cuvettes (G01N21/09 takes precedence; handling fluid samples G01N1/10) · CPC title

• B01L3/502715 Primary

  characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces · CPC title

• C12M35/02 Primary

  Electrical or electromagnetic means, e.g. for electroporation or for cell fusion · CPC title

• C12N13/00

  Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves · CPC title