Method for enhancing red blood cell quality and survival during storage

Patent metadata

Field	Value
Publication number	US-10251387-B2
Application number	US-201615095943-A
Country	US
Kind code	B2
Filing date	Apr 11, 2016
Priority date	Aug 25, 2010
Publication date	Apr 9, 2019
Grant date	Apr 9, 2019

Abstract

The present invention is a method for enhancing the quality and survival of red blood cells during storage by depleting the red blood cells of both carbon dioxide and oxygen and maintaining 2,3-diphosphoglycerate acid levels.

First claim

What is claimed is:

1. A method for enhancing red blood cell quality and survival during storage comprising
depleting a red blood cell sample of leukocytes;
depleting said leukocyte depleted red blood sample of platelets;
adding an additive solution having a pH ranging from 5.5 to 7.0;
depleting said leukoreduced and platelet reduced red blood cell sample of both oxygen and carbon dioxide prior to storage, wherein carbon dioxide is depleted to a level of 5 mmHg at 21-25° C.;
reducing pathogens in said red blood cell sample; and
transferring the leukocyte, platelet, pathogen and oxygen- and carbon dioxide-depleted red blood cell sample to an oxygen- and carbon dioxide-impermeable environment for storage, wherein said red blood cell sample has an acidic pH,
thereby enhancing red blood cell quality and survival during storage.

2. The method of claim 1 , wherein 2,3-diphosphoglycerate (2,3-DPG) acid levels are maintained for at least two weeks.

3. The method of claim 1 , wherein the red blood cell sample is stored for at least three weeks.

4. The method of claim 3 , wherein the red blood cells exhibit less than 0.2% hemolysis.

5. The method of claim 1 , wherein the red blood cells sample is stored for at least seven weeks.

6. The method of claim 5 , wherein the red blood cells exhibit less than 0.3% hemolysis.

7. The method of claim 1 , wherein the red blood cells sample is stored for at least nine weeks.

8. The method of claim 7 , wherein the red blood cells exhibit less than 0.7% hemolysis.

9. The method of claim 1 , wherein the red blood cell sample is depleted of oxygen to a level of approximately 10 mmHg at 21-25° C.

10. The method of claim 1 , wherein said additive solution has a pH ranging from 6.25 to 6.75.

11. The method of claim 1 , wherein the oxygen- and carbon dioxide-impermeable environment for storage is between 1° C. and 6° C.

12. The method of claim 1 , wherein the red blood cell sample is selected from the group consisting of whole blood, anti-coagulated whole blood, packed red cells and red cells separated from plasma.

13. A method for enhancing red blood cell quality and survival during storage comprising
(a) reducing oxygen and carbon dioxide in a red blood cell sample comprising an acidified additive solution prior to storage;
(b) reducing pathogens; and
(c) storing the oxygen and carbon dioxide reduced red blood cell sample in an oxygen and carbon dioxide-impermeable storage environment, wherein said red blood cell sample has an acidic pH,
wherein adenosine triphosphate (ATP) and 2,3-diphosphoglycerate (2,3-DPG) levels are optimized during storage in the oxygen- and carbon dioxide-impermeable storage environment.

14. The method of claim 13 , wherein the red blood cell sample is depleted of oxygen to a level of approximately 10 mmHg at 21-25° C.

15. The method of claim 13 , wherein the red blood cell sample is depleted of carbon dioxide to a level of 5 mmHg at 21-25° C.

16. The method of claim 13 , wherein reducing carbon dioxide in the red blood cell sample elevated 2,3-DPG levels relative to a red blood cell sample in which carbon dioxide was not depleted.

17. The method of claim 13 , wherein reducing carbon dioxide and oxygen in the red blood cell sample maintains ATP levels higher for a nine week period than ATP levels in a red blood cell sample in which neither oxygen nor carbon dioxide are depleted and maintains 2,3-DPG levels higher for three weeks than 2,3-DPG levels in a red blood cell sample in which neither oxygen nor carbon dioxide are depleted.

Assignees

• New Health Sciences Inc
• Dartmouth College

Inventors

• Dumont Larry J
• Yoshida Tatsuro

Classifications

• A61M1/0272

  Apparatus for treatment of blood or blood constituents prior to or for conservation, e.g. freezing, drying or centrifuging · CPC title

• A01N1/021 Primary

  Human Necessities · mapped topic

• A01N1/0242

  Human Necessities · mapped topic

• C12N5/0641

  Erythrocytes · CPC title

• A01N1/142

  Apparatus · CPC title