Method for diagnosing breast cancer

Patent metadata

Field	Value
Publication number	US-10246749-B2
Application number	US-201615075181-A
Country	US
Kind code	B2
Filing date	Mar 20, 2016
Priority date	Mar 20, 2015
Publication date	Apr 2, 2019
Grant date	Apr 2, 2019

Abstract

The invention relates to a method of diagnosing breast cancer and to the use of biomarkers for the detection and diagnosis of breast cancer.

First claim

What is claimed:

1. A method of diagnosing a subject with breast cancer, said method comprising the steps of:
a. detecting and measuring the levels of the following four miR gene products: miR-21, miR125b, miR-451, and miR-155 in a cell-free test sample isolated from a whole urine sample obtained from said subject;
b. comparing the respective levels of each of said four miR gene products in the subject test sample detected and measured in step (a) with its corresponding miR gene product control level in a control sample; and
c. diagnosing with breast cancer the subject for whom the comparison of step (b) reveals a decrease in the respective levels of miR-21, miR-125b, and miR-451, and an increase in the level of miR-155 in the subject test sample measured in step (a) as compared to each corresponding control level,
wherein said cell-free test sample is a supernatant isolated from the whole urine sample, wherein the whole urine sample has been processed to eradicate contamination with any urothelial or microbiological cell material.

2. The method of claim 1 , wherein said subject test sample is contacted with (i) an oligonucleotide capable of hybridizing under high stringency conditions to a nucleic acid consisting of SEQ ID NO:1, (ii) an oligonucleotide capable of hybridizing under high stringency conditions to a nucleic acid consisting of SEQ ID NOs: 3 or 9; (iii) an oligonucleotide capable of hybridizing under high stringency conditions to a nucleic acid consisting of SEQ ID NO: 5; and (iv) an oligonucleotide capable of hybridizing under high stringency conditions to a nucleic acid consisting of SEQ ID NO: 7.

3. The method of claim 1 , wherein the levels of each of said four miR gene products are measured by quantitative RT-PCR.

4. The method of claim 1 , wherein the levels of each of said four miR gene products are measured by hybridization using an oligonucleotide microarray.

5. The method of claim 4 , wherein said oligonucleotide microarray comprises:
a. an oligonucleotide capable of hybridizing under high stringency conditions with a nucleic acid consisting of SEQ ID NO: 2;
b. an oligonucleotide capable of hybridizing under high stringency conditions with a nucleic acid consisting of SEQ ID NO: 4;
c. an oligonucleotide capable of hybridizing under high stringency conditions with a nucleic acid consisting of SEQ ID NO: 6; and
d. an oligonucleotide capable of hybridizing under high stringency conditions with a nucleic acid consisting of SEQ ID NO: 8.

6. The method of claim 4 , wherein said oligonucleotide microarray comprises:
a. an oligonucleotide perfectly complementary to a nucleic acid consisting of SEQ ID NO: 2;
b. an oligonucleotide perfectly complementary to a nucleic acid consisting of SEQ ID NO: 4;
c. an oligonucleotide perfectly complementary to a nucleic acid consisting of SEQ ID NO: 6; and
d. an oligonucleotide perfectly complementary to a nucleic acid consisting of SEQ ID NO: 8.

7. The method of claim 1 , wherein the control sample is a urine sample obtained from one or more healthy, cancer-free individuals.

8. The method of claim 1 , wherein the subject is a female human.

9. The method of claim 1 , wherein the levels of the at least three each of said four miR gene products are measured by hybridization using an oligonucleotide microarray that comprises:
a. an oligonucleotide capable of hybridizing under high stringency conditions with a nucleic acid consisting of SEQ ID NO: 2;
b. an oligonucleotide capable of hybridizing under high stringency conditions with a nucleic acid consisting of SEQ ID NO: 4;
c. an oligonucleotide capable of hybridizing under high stringency conditions with a nucleic acid consisting of SEQ ID NO: 6;
d. an oligonucleotide capable of hybridizing under high stringency conditions with a nucleic acid consisting of SEQ ID NO: 8.

10. The method of claim 1 , wherein the levels of the at-least-three each of said four miR gene products are measured by hybridization using an oligonucleotide microarray, further wherein said oligonucleotide microarray comprises at least three of the following:
a. an oligonucleotide perfectly complementary to a nucleic acid consisting of SEQ ID NO: 2;
b. an oligonucleotide perfectly complementary to a nucleic acid consisting of SEQ ID NO: 4;
c. an oligonucleotide perfectly complementary to a nucleic acid consisting of SEQ ID NO: 6; and
d. an oligonucleotide perfectly complementary to a nucleic acid consisting of SEQ ID NO: 8.

11. The method of claim 1 , wherein said step c) of diagnosing said subject with breast cancer requires a decrease of 25% or more in the level of the miR-21 gene product, the miR-125b gene product, and the miR-451 gene product in the subject test sample, relative to the respective control levels of the corresponding gene products, coupled with an increase of 25% of more in the level of the miR-155 gene product in the subject test sample, relative to the control level of the miR-155 gene product.

12. The method of claim 1 , wherein said step c) of diagnosing said subject with breast cancer requires that:
a. the level of the miR-21 gene product in the test sample from the subject is less than 70% of the level of miR-21 gene product in the control sample;
b. the level of the miR-125 b gene product in the test sample from the subject is less than 70% of the level of miR-125b gene product in the control sample;
c. the level of the miR-451 gene product in the test sample from the subject is less than 60% of the level of miR-451 gene product in the control sample;
d. the level of the miR-155 gene product in the test sample from the subject is more than 300% of the level of miR-155 gene product in the control sample.

13. A method of treating breast cancer in a subject in need thereof, said method comprising the steps of:
a. in a cell free test sample isolated from a whole urine sample obtained from the subject, detecting and measuring the respective levels of the following four miR gene products: miR-21, miR-125b, miR-451, and miR-155;
b. comparing the levels of each of said four miR gene products in the subject test sample measured in step (a) with its corresponding miR gene product control level in a control sample; and
c. administering a chemotherapeutic regimen suitable for treating breast cancer to the subject for whom the comparison of step (b) reveals a decrease in the level of the miR-21 gene product, the miR-125b gene product, and the miR-451 gene product in the subject test sample, relative to the corresponding control level for the respective gene product(s) and reveals an increase in the level of the miR-155 gene product in the subject test sample, relative to the corresponding control level for the respective gene product(s),
wherein said cell-free test sample is a supernatant isolated from the whole urine sample, wherein the whole urine sample has been processed to eradicate contamination with any urothelial or microbiological cell material.

14. The method of claim 1 , wherein the levels of each of said four miR gene products are measured by hybridization using an oligonucleotide microarray comprising at least four probe oligonucleotides, each of which is specific for one of said four miR gene products.

15. The method of claim 1 , wherein said supernatant is isolated from said whole urine sample by means of centrifugation.

16. The method of claim 13 , further comprising the step of repeating method steps (a) and (b) at regular time intervals in those subjects administered a chemotherapeutic regimen suitable for breast cancer in accordance with step (c) in order to monitor th

Assignees

• Univ Freiburg Albert Ludwigs

Inventors

• Stickeler Elmar
• Erbes Thalia
• Jaeger Markus

Classifications

• C12Q2600/158

  Expression markers · CPC title

• C12Q2600/178

  miRNA, siRNA or ncRNA · CPC title

• C12Q1/6886 Primary

  for cancer (immunoassay for cancer G01N33/575) · CPC title