Film sensor

Patent metadata

Field	Value
Publication number	US-10241099-B2
Application number	US-201515125405-A
Country	US
Kind code	B2
Filing date	Mar 19, 2015
Priority date	Mar 20, 2014
Publication date	Mar 26, 2019
Grant date	Mar 26, 2019

Abstract

The present disclosure relates to a sensor for indicating food quality comprising a semi-permeable film layer, the semi-permeable film layer comprising at least one integrally formed well having at least one sensing element disposed therein; wherein the well is sealed by a second film layer, the semi-permeable film layer being impermeable to said sensing element but is permeable to at least one analyte detectable by said sensing element.

First claim

The invention claimed is:

1. A sensor comprising a semi-permeable film layer, said semi-permeable film layer comprising at least one integrally formed well having at least one sensing element disposed therein; wherein the well is sealed by a second film layer, said semi-permeable film layer being impermeable to said sensing element but is permeable to at least one analyte detectable by said sensing element, wherein said semi-permeable film layer comprises at least two or more polyelectrolyte layers, and wherein oppositely charged polyelectrolyte layers are deposited alternately on each other.

2. The sensor of claim 1 , wherein the semi-permeable layer comprises an array of integrally formed wells, wherein at least one sensing element or a mixture of sensing elements is encapsulated in each well.

3. The sensor of claim 2 , wherein said mixture of sensing elements comprises two or more distinct sensing elements adapted to detect the same or different analytes.

4. The sensor of claim 1 , wherein at least one well contains at least two-distinct sensing elements, each distinct sensing element adapted to detect the presence of at least one or more analytes.

5. The sensor of claim 1 , wherein the molecular weight cut-off (MWCO) of the semi-permeable layer is from about 1 to 20 kDa.

6. The sensor of claim 1 , wherein the analytes are chemical or biochemical markers produced during muscle cell autolysis or bacterial growth.

7. The sensor of claim 1 , wherein the analytes are selected from the group consisting of: H + ions, hypoxanthine, inosine, inosine monophosphate, n-butyrate, L-lactic acid, D-lactate, acetic acid, ethanol, and biogenic amines.

8. The sensor of claim 7 , wherein the analytes are selected from the group consisting of: hypoxanthine, inosine and inosine monophosphate.

9. The sensor of claim 1 , wherein said sensing elements provide at least one measurable property upon detecting said analytes.

10. The sensor of claim 9 , wherein the measurable property is fluorescence.

11. The sensor of claim 1 , wherein at least one well contains a sensing element capable of detecting a change in pH and at least one well contains a sensing element capable of detecting hydrogen peroxide.

12. The sensor of claim 1 , wherein the sensing elements are selected from the group consisting of: fluorescent dyes and enzymes.

13. The sensor of claim 1 , wherein said second film layer is optically translucent or transparent.

14. This sensor of claim 13 , wherein said second film layer is a semi-permeable layer.

15. This sensor of claim 14 , wherein said second film layer is not permeable to said sensing element but is permeable to said analytes.

16. The sensor of claim 1 , wherein said second film layer comprises at least one exposed surface not engaged with said semi-permeable film layer, said exposed surface being an adhesive surface or comprising means for adhesion to a substrate surface.

17. The sensor of claim 16 , wherein said exposed surface comprises an adhesive layer.

18. A method of assessing food quality in packaged food, the method comprising the steps of: a) contacting a sensor according to claim 1 with said food; b) detecting at least one measurable output from said sensor.

19. A method of making a sensor, said method comprising the steps of:
depositing a semi-permeable film coating on a patterned template to thereby form a semi-permeable film layer having at least one integrally formed well;
disposing one or more sensing elements in said well; and
encapsulating said sensing elements in said well;
wherein said semi-permeable film layer is not permeable to said sensing element but is permeable to at least one analyte detectable by said sensing element, wherein said semi-permeable film layer comprises at least two or more polyelectrolyte layers, and wherein oppositely charged polyelectrolyte layers are deposited alternately on each other.

Assignees

• Agency Science Tech & Res
• Univ Massey

Inventors

• Kiryukhin Maxim
• Sadovoy Anton
• Korzh Vladimir
• Teh Cathleen
• Singh Harjinder
• Lad Mita

Classifications

• G01N31/223

  for investigating presence of specific gases or aerosols (G01N31/221, G01N31/222 take precedence; actuation of fire alarm by presence of smoke or gases G08B17/10) · CPC title

• G01N2021/6439

  with indicators, stains, dyes, tags, labels, marks · CPC title

• G01N33/12 Primary

  Meat; Fish · CPC title

• G01N21/6428

  Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53) · CPC title

• G01N21/80

  Indicating pH value · CPC title