Apparatus and methods for detecting ATP in a liquid sample

Patent metadata

Field	Value
Publication number	US-10240181-B2
Application number	US-201314412143-A
Country	US
Kind code	B2
Filing date	Feb 25, 2013
Priority date	Jul 6, 2012
Publication date	Mar 26, 2019
Grant date	Mar 26, 2019

Abstract

Apparatuses and kits for the detection of ATP in a liquid sample are provided. The apparatuses and kits comprise a liquid reagent composition comprising luciferin and a sampling device having a sampling portion and a handling portion. The sampling portion comprises a fibrous or a foam material and is adapted to acquire and releasably retain a predetermined volume of a liquid sample or a sample of residue from a surface. The sampling device comprises a dry coating or a liquid coating, the coating including an effective amount of a pH-adjusting reagent that, when contacted with a liquid reagent composition having a pH 6.8 or lower, changes the pH of the liquid reagent composition to 6.9 or higher. Methods of use of the apparatus or kit are also provided.

First claim

What is claimed is:

1. A method, comprising:
using a sampling device to obtain a predetermined volume of sample;
wherein the sampling device comprises a sampling portion that comprises a fibrous material or a foam material;
wherein the sampling device comprises a dry coating that includes an effective amount of a pH-adjusting reagent that, when contacted with a liquid reagent composition having a pH of about 6.8 or lower, changes the pH of the liquid reagent composition to 6.9 or higher;
wherein the liquid reagent composition comprises a luciferin;

contacting the predetermined volume of sample with both the dry coating comprising the pH-adjusting reagent and the liquid reagent composition in a container to form a reaction mixture; and
using a luminometer to detect light emitted from the reaction mixture;
wherein the container comprises a cuvette portion adapted to be operationally coupled to the luminometer.

2. The method of claim 1 , further comprising the step of agitating the sampling portion and the liquid reagent composition in the container.

3. The method of claim 1 , wherein the liquid reagent composition further comprises a luciferase enzyme.

4. The method of claim 1 , wherein contacting the sampling device comprising the sample with liquid reagent composition comprises contacting the sampling device with the liquid reagent composition at a temperature within a range of 10° C. and 35° C., inclusive.

5. The method of claim 1 wherein, after contacting the sampling device with liquid reagent composition, a steady-state amount of light is emitted from the composition in less than 20 seconds.

Assignees

• 3M Innovative Properties Co
• Driscoll Mark B
• Evans Rachel E
• Ramsay Catherine M
• Percy Neil

Inventors

• Driscoll Mark B
• Evans Rachel E
• Ramsay Catherine M
• Percy Neil

Classifications

• C12Q1/008 Primary

  for determining co-enzymes or co-factors, e.g. NAD, ATP · CPC title

• C12Q1/66

  involving luciferase · CPC title