Capillary barriers for staged loading of microfluidic devices

Patent metadata

Field	Value
Publication number	US-10233441-B2
Application number	US-201715853370-A
Country	US
Kind code	B2
Filing date	Dec 22, 2017
Priority date	Mar 14, 2013
Publication date	Mar 19, 2019
Grant date	Mar 19, 2019

Abstract

Various aspects of the present disclosure are directed toward methods and apparatuses for interacting a first liquid and a second liquid in one or more fluidic channels of a capillary structure. The methods and apparatuses can include providing at least one capillary barrier that positions a meniscus of the first liquid at a fluid-interface region using capillary forces within the capillary structure. Additionally, a path is provided along one of the channels for the second liquid to flow toward the fluid-interface region. Additionally, gas pressure is released, via a gas-outflow port, from the fluid-interface region while flow of the first liquid is arrested. Further, the first liquid and the second liquid contact in the fluid-interface region with the capillary barrier holding the first liquid at the fluid-interface region.

First claim

What is claimed is:

1. An apparatus comprising:
a fluidic system including a first microfluidic channel configured and arranged to guide a first liquid toward a fluid-interface region and including a second microfluidic channel configured and arranged to guide a second liquid toward the fluid-interface region;
a capillary barrier configured and arranged to arrest flow of the first liquid at the fluid-interface region using capillary forces, wherein the capillary barrier is at least in part defined by a change in a cross-sectional area, the change in the cross-sectional area being sufficient to arrest flow of the first liquid at the fluid-interface region;
a second capillary barrier configured and arranged to arrest flow of the second liquid at the fluid-interface region using capillary forces thereby to preserve an interface between the first liquid and the second liquid; and
an escape path through which fluid can escape from the fluid-interface region.

2. The apparatus of claim 1 , wherein the first liquid and the second liquid are loaded without using a vacuum.

3. The apparatus of claim 1 , wherein the first microfluidic channel and the second microfluidic channel are formed from a polymeric material.

4. The apparatus of claim 1 , wherein the first microfluidic channel and the second microfluidic channel respectively include hydrophobic surfaces.

5. The apparatus of claim 1 , further including:
a microfluidic chip configured to interface or interact with fluid, and
circuitry configured and arranged to receive data indicative of an interaction between the first liquid and the second liquid and to perform microfluidics analysis of quantification and quality control of one or more of DNA, RNA, proteins, and cells in at least one of the first liquid and the second liquid.

6. The apparatus of claim 1 , further including;
a microfluidic chip configured to interface or interact with fluid; and
circuitry configured and arranged to receive data indicative of an interaction between the first liquid and the second liquid, and to perform analysis of electrophoresis, analysis of separation, or analysis of extraction of DNA fragments in at least one of the first liquid and the second liquid.

7. The apparatus of claim 1 , further including:
a microfluidic chip configured to interface or interact with fluid; and
circuitry configured and arranged to receive data indicative of an interaction between the first liquid and the second liquid, and to perforin analysis of enrichment of target nucleic acid in at least one of the first liquid and the second liquid.

8. The apparatus of claim 1 , further including a gas-outflow port configured and arranged to release gas pressure while flow of the first liquid is arrested, and wherein during operation, the second capillary barrier arrests flow of the second liquid at the fluid-interface region and a liquid-liquid interface is formed.

9. The apparatus of claim 8 , wherein the gas-outflow port is configured and arranged to permit release of air while flow of the first liquid is arrested.

10. The apparatus of claim 1 , wherein the escape path includes a region for limiting a volume flow rate or a volume of the fluid; and the escape path is connected to a gas-outflow port configured and arranged to release gas pressure while flow of the first liquid is arrested.

11. The apparatus of claim 10 , wherein the region for limiting a volume flow rate or a volume of the fluid includes an expansion of the cross-sectional area.

12. The apparatus of claim 1 , wherein the change of the cross-sectional area is an expansion of the cross-sectional area.

13. The apparatus of claim 1 , wherein the capillary barrier includes a protrusion, ramp, or post included on a wall of the first microfluidic channel upstream of the fluid-interface region.

14. The apparatus of claim 1 , wherein the escape path includes a low volume channel, a constricted channel, a protrusion, a ramp or a post.

15. The apparatus of claim 1 , wherein the escape path includes a region of the capillary barrier is configured and arranged to decrease dimensions of the cross-sectional area of the escape path.

16. The apparatus of claim 1 , wherein the fluid escapes from the fluid-interface region and through the escape path is a gas.

17. The apparatus of claim 1 , wherein the escape path further comprises the second capillary barrier, and wherein the second capillary barrier is further configured and arranged to arrest flow of the second liquid at the fluid-interface region, wherein an interface between the first liquid and the second liquid results and a meniscus of the second liquid is maintained.

18. The apparatus of claim 1 , wherein the escape path is configured and arranged to limit fluid flow due to increased hydraulic resistance in the escape path.

19. The apparatus of claim 1 , wherein the second capillary barrier is further configured and arranged to arrest flow of the second liquid at the fluid-interface and to fat n a stationary interface or front where the first liquid meets the second liquid.

20. A method for interacting a first liquid and a second liquid in one or more fluidic channels, the method comprising:
providing a capillary barrier to position a meniscus of the first liquid at a fluid-interface region using capillary forces;
providing a path along one of the channels for the second liquid to flow toward the fluid-interface region;
providing a second capillary barrier to position a meniscus of the second liquid at the fluid-interface region;
providing an escape path for escaping fluid to exit from the region between the first and second liquid;
limiting a volume flow rate or a volume of the escaping fluid;
arresting flow of the second liquid at the fluid-interface region with the second capillary barrier; and
causing the first liquid and the second liquid to contact in the fluid-interface region thereby preserving an interface between the first liquid and the second liquid.

21. The method of claim 20 , further including a gas-outflow port connected to the escape path, wherein the first liquid and the second liquid merge in the fluid-interface region.

22. A method for interacting a first liquid and a second liquid in one or more fluidic channels, the method comprising:
providing a capillary barrier to position a meniscus of the first liquid at a fluid-interface region using capillary forces;
providing a path along one of the channels for the second liquid to flow toward the fluid-interface region;
providing a second capillary barrier to position a meniscus of the second liquid at the fluid-interface region;
using the second capillary barrier and capillary forces to arrest flow of the second liquid at the fluid-interface region; and
causing the first liquid and the second liquid to contact in the fluid-interface region.

23. The method of claim 22 , further including providing an escape path for escaping fluid to exit from the region between the first and second liquid, and limiting a volume flow rate or a volume of the escaping fluid.

Assignees

• Univ Leland Stanford Junior

Inventors

• Santiago Juan G
• Marshall Lewis A
• Rogacs Anita

Classifications

• B01L3/502723 Primary

  characterised by venting arrangements · CPC title

• B01L2200/0631

  Purification arrangements, e.g. solid phase extraction [SPE] · CPC title

• B01L2200/0621

  Control of the sequence of chambers filled or emptied · CPC title

• B01L3/502738

  characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746) · CPC title

• C12Q1/6806

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title