Control of chemical reactions using isotachophoresis

Patent metadata

Field	Value
Publication number	US-9297039-B2
Application number	US-201414465138-A
Country	US
Kind code	B2
Filing date	Aug 21, 2014
Priority date	Oct 7, 2008
Publication date	Mar 29, 2016
Grant date	Mar 29, 2016

Abstract

Isotachophoresis (ITP) is exploited to control various aspects of chemical reactions. In a first aspect, at least one of the reactants of a chemical reaction is confined to an ITP zone, but the resulting product of the chemical reaction is separated from this ITP zone by the ITP process. In a second aspect, one or more reactants of a chemical reaction are confined to an ITP zone, and one or more other reactants of the chemical reaction are not confined to this ITP zone. In a third aspect, ITP is employed to confine at least one reactant of a chemical reaction to an ITP zone, and at least one reactant of the chemical reaction is delivered to the ITP zone in two or more discrete doses. These aspects are especially relevant to performing polymerase chain reactions using chemical denaturants as opposed to thermal cycling.

First claim

The invention claimed is:

1. A method of performing a chemical reaction, the method comprising:
providing at least a first reactant and a second reactant, wherein the first reactant and the second reactant are confined by isotachophoresis (ITP) to a first ITP zone in a liquid flow channel;
providing a catalyst or enzyme in said first ITP zone;
allowing said first and second reactants to react in said first ITP zone to provide at least a first product, wherein the catalyst or enzyme facilitates production of said first product via one or more chemical reactions, and wherein the catalyst or enzyme is not consumed in the one or more chemical reactions.

2. The method of claim 1 , wherein said first ITP zone is substantially stationary in said liquid flow channel.

3. The method of claim 1 , further comprising monitoring a concentration of at least one of said reactants or said first product.

4. The method of claim 1 , wherein said chemical reaction is part of a polymerase chain reaction, wherein said first reactant comprises nucleic acid, and wherein said second reactant comprises primers or oligonucleotides.

5. The method of claim 1 , wherein said first product is confined by said ITP to said first ITP zone.

6. The method of claim 1 , wherein said enzyme or catalyst comprises polymerase.

7. The method of claim 4 , wherein said first product comprises a deoxyribonucleic acid.

8. The method of claim 1 , wherein the chemical reaction is performed at constant temperature.

9. The method of claim 1 , wherein the chemical reaction is performed at one or more temperatures that are elevated above an ambient temperature.

Assignees

• Univ Leland Stanford Junior

Inventors

• Santiago Juan G
• Persat Alexandre

Classifications

• C12Q1/686 Primary

  Polymerase chain reaction [PCR] · CPC title

• B01D57/02

  by electrophoresis (treatment of water, waste water, sewage or sludge by electrophoresis C02F1/469; electrophoretic production of compounds or non-metals C25B7/00; investigating or analysing materials by using electrophoresis G01N27/26) · CPC title

• C12Q2527/125

  Specific component of sample, medium or buffer · CPC title

• C12Q2565/629

  being a microfluidic device · CPC title

• C12M1/42

  Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves · CPC title