Coriolus versicolor extracts, methods of preparation and uses thereof

Patent metadata

Field	Value
Publication number	US-10226493-B2
Application number	US-201113876070-A
Country	US
Kind code	B2
Filing date	Oct 6, 2011
Priority date	Oct 6, 2010
Publication date	Mar 12, 2019
Grant date	Mar 12, 2019

Abstract

The subject invention provides Coriolus versicolor extracts, pharmaceutical compositions comprising the Coriolus versicolor extracts, methods of preparation, and therapeutic uses thereof. Advantageously, the subject Coriolus versicolor extract has immunomodulatory, anti-tumor, anti-microbial, and antiviral effects. In a preferred embodiment, the subject invention can be used to inhibit the metastatic spread of cancer cells. In certain preferred embodiments, the subject invention can be used to treat glioblastoma multiforme, nasopharyngeal carcinoma, breast carcinoma, lung carcinoma, prostate cancer, and colon carcinoma, as well as bacterial, viral, fungal, protozoan, and/or other microbial infection.

First claim

We claim:

1. A method for treating a disease or condition selected from brain tumors; nasopharyngeal carcinomas; breast cancer; lung cancer; leukemia; lymphoma; colon cancer; stomach cancer; esophageal cancer; bladder cancer; gastric cancer; glioblastoma multiform; prostate cancer; and viral, bacterial, protozoan, fungal and microbial infections where the modulation of an immune response would be beneficial, wherein said method comprises administering, to a subject in need of such treatment, an effective amount of a composition comprising a Coriolus versicolor extract obtained by either:
A) a method comprising the steps of:
a) providing a sufficient quantity of raw material of Coriolus versicolor ; and
b) extracting biologically-active chemical constituents from the raw material of Coriolus versicolor with a polar solvent that is about 95% ethanol at a temperature of about 15° C. to about 30° C. to yield a Coriolus versicolor ethanol extract and a residue, and recovering the Coriolus versicolor ethanol extract;
wherein step b) is performed once or more than once; or
B) a method comprising the steps of:
a) providing a sufficient quantity of raw material of Coriolus versicolor;
b) extracting biologically-active chemical constituents from the raw material of Coriolus versicolor with a polar solvent that is about 95% ethanol to yield a Coriolus versicolor ethanol extract and a residue, and recovering the Coriolus versicolor ethanol extract, wherein step b) is performed once or more than once;
c) extracting the residue obtained in step b) with an aqueous alkaline solution to yield an aqueous extract, and recovering said aqueous extract, wherein step c) is performed once or more than once; and
d) combining one or more extracts obtained from steps b) and c) to yield Coriolus versicolor extract.

2. The method, according to claim 1 , used to reduce IL-10 production and/or increase IFNβ production.

3. The method, according to claim 1 , used to treat a brain tumor, nasopharyngeal carcinoma, breast cancer, lung cancer, leukemia, lymphoma, colon cancer, stomach cancer, esophageal cancer, bladder cancer, or gastric cancer.

4. The method, according to claim 1 , used to treat glioblastoma multiforme, nasopharyngeal carcinoma, breast carcinoma, lung carcinoma, prostate cancer, or colon carcinoma.

5. The method, according to claim 1 , used to treat infection by human immunodeficiency virus (HIV), influenza A virus, influenza B virus, human T-lymphotropic virus Type I (HTLV-1), bovine leukemia virus (BLV), Epstein-Barr virus, coronavirus, mycobacteria, Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae, Escherichii coli, Listeria monocytogenes, L. amazonensis, Pneumocystis, Toxoplasma, Candida , and/or Aspergillus.

6. The method, according to claim 1 , used to treat infection by herpes simplex virus, varicella zoster, cytomegalovirus, and/or herpes virus-8.

Assignees

• Chik Stanley Chi Chung
• Law Anna Hing Yee
• Yang Cindy Lai Hung
• Lau Maureen
• Bagi Res Limited
• Versitech Ltd

Inventors

• Lau Allan Sik Yin
• Chik Stanley Chi Chung
• Law Anna Hing-Yee
• Yang Cindy Lai Hung

Classifications

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P37/04

  Immunostimulants · CPC title

• A61P37/02

  Immunomodulators · CPC title

• A61P35/02

  specific for leukemia · CPC title

• A61P35/00

  Antineoplastic agents · CPC title