Ebolavirus pre-hairpin intermediate mimics and methods of use

Patent metadata

Field	Value
Publication number	US-10189878-B2
Application number	US-201515513959-A
Country	US
Kind code	B2
Filing date	Sep 24, 2015
Priority date	Sep 24, 2014
Publication date	Jan 29, 2019
Grant date	Jan 29, 2019

Abstract

Ebolavirus is a highly lethal filovirus that causes hemorrhagic fever in humans and non-human primates. With no approved treatments or preventatives, the development of an anti- ebolavirus therapy to protect against natural infections and potential weaponization is an urgent unmet global health need. The design, biophysical characterization, and validation of peptide mimics of the ebolavirus N-trimer (“N-trimer mimics”) are described herein.

First claim

The invention claimed is:

1. An Ebolavirus N-trimer mimic comprising a homotrimer of peptide monomers, wherein each peptide monomer comprises an an amino acid sequence of SEQ ID NO:23 or SEQ ID NO:21.

2. The Ebolavirus N-trimer mimic of claim 1 , wherein each peptide monomer comprises an amino acid sequence of SEQ ID NO:23.

3. The Ebolavirus N-trimer mimic of claim 1 , wherein each peptide monomer comprises an amino acid sequence of SEQ ID NO:21.

4. A pharmaceutical composition comprising an Ebolavirus N-trimer mimic of claim 1 , and a pharmaceutically acceptable carrier.

5. A method of inhibiting Ebolavirus entry into a cell exposed to Ebolavirus , the methods comprising delivering an Ebolavirus N-trimer mimic of claim 1 to the cell exposed to Ebolavirus.

6. The method of claim 5 , wherein the cell is human.

Assignees

• Univ Utah Res Found
• Clinton Tracy R
• Navigen Inc
• The United States of America—Air Force

Inventors

• Clinton Tracy R
• Jacobsen Michael Thomas
• Weinstock Matthew T
• Welch Brett D
• Eckert Debra Muir
• Kay Michael S

Classifications

• C07K14/08 Primary

  RNA viruses · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title

• C12N2760/14133

  Use of viral protein as therapeutic agent other than vaccine, e.g. apoptosis inducing or anti-inflammatory · CPC title

• G01N2333/08

  RNA viruses · CPC title

• G01N2500/04

  Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A) · CPC title