Biocompatible substrate for facilitating interconnections between stem cells and target tissues and methods for implanting same

Patent metadata

Field	Value
Publication number	US-10188769-B2
Application number	US-201414314994-A
Country	US
Kind code	B2
Filing date	Jun 25, 2014
Priority date	Jul 12, 2010
Publication date	Jan 29, 2019
Grant date	Jan 29, 2019

Abstract

Disclosed herein are substrates for cell delivery to target tissues requiring treatment for various diseases that induce cell death, damage or loss of function. The substrates are configured to provide seeded cells, including stem cells, with a structural support that allows interconnection with and transmission of biological signals between the cells and the target tissue.

First claim

What is claimed is:

1. A method for treating retinal degenerative disease comprising:
providing a substrate seeded with cells suitable for implantation into an ocular tissue of a patient, wherein the substrate comprises:
a parylene layer with an apical surface for growth of a population of cells, wherein the parylene layer has a thickness between 0.1 microns and 6 microns;
a plurality of supporting protrusions each extending 1 micron or greater from a basal surface of the parylene layer to a terminating free end of the supporting protrusion;

implanting the substrate into the ocular tissue of the patient; and
positioning the substrate so that the substrate is parallel with an outer nuclear layer of photoreceptors in an eye of the patient.

2. The method of claim 1 , wherein the substrate is implanted through an ab-interno surgical approach.

3. The method of claim 1 , wherein the substrate is implanted through an ab-externo surgical approach.

4. The method of claim 1 , wherein the substrate is implanted into a subretinal space of the eye of the patient.

5. The method of claim 1 , wherein the substrate is positioned with the apical surface adjacent to an epiretinal side of a retina of the eye of the patient.

6. The method of claim 1 , wherein the substrate is implanted during an open surgical process.

7. The method of claim 1 , wherein the substrate is implanted endoscopically.

8. The method of claim 1 , wherein the substrate is positioned substantially parallel to a posterior eye wall of the patient.

9. The method of claim 1 , wherein the substrate is loaded onto a surgical introducer prior to implantation.

10. The method of claim 1 , wherein the supporting protrusions are comprised of parylene.

11. The method of claim 1 , wherein the supporting protrusions are distributed in a periodic pattern on the basal surface, columnar in shape, and distributed longitudinally along a long axis of the basal surface, wherein the basal surface is configured to prevent growth of blood vessels through the basal surface to the apical surface.

12. The method of claim 1 , wherein the substrate further comprises a handle portion protruding laterally from the substrate.

13. The method of claim 12 , wherein the handle portion protrudes from an oval shaped portion of the substrate.

14. The method of claim 12 , wherein a length of the handle portion is between 1 mm and 20 mm.

15. The method of claim 14 , wherein a length of the handle portion is between 1 mm and 2 mm.

16. The method of claim 12 , wherein a width of the handle portion is between 0.2 mm and 6 mm.

17. The method of claim 12 , wherein the handle portion was formed by cutting.

18. The method of claim 1 , wherein the substrate further comprises a raised lip surrounding the apical surface.

19. The method of claim 18 , wherein the raised lip has a height ranging from 10 microns to 1000 microns and a width ranging from 10 microns to 1000 microns.

20. The method of claim 1 , wherein each supporting protrusion extends from the parylene layer to the free end such that a height of the substrate from the apical surface to the free end of each supporting protrusion is 3 microns or greater.

Assignees

• Univ Southern California
• California Inst Of Techn
• Univ California

Inventors

• Humayun Mark S
• Ahuja Ashish
• Tai Yu-Chong
• Hinton David R
• Grubbs Robert H
• Clegg Dennis O
• Johnson Lincoln Vallance
• Hikita Sherry T

Classifications

• C12N2535/00

  Supports or coatings for cell culture characterised by topography · CPC title

• A61L27/3813

  Epithelial cells, e.g. keratinocytes, urothelial cells · CPC title

• A61P27/00

  Drugs for disorders of the senses · CPC title

• A61L27/34

  Macromolecular materials · CPC title

• A61L27/18 Primary

  obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds · CPC title