3-dimensional parylene scaffold cage

Patent metadata

Field	Value
Publication number	US-9642940-B2
Application number	US-201615004796-A
Country	US
Kind code	B2
Filing date	Jan 22, 2016
Priority date	Dec 5, 2011
Publication date	May 9, 2017
Grant date	May 9, 2017

Abstract

Thin parylene C membranes having smooth front sides and ultrathin regions (e.g., 0.01 μm to 5 μm thick) interspersed with thicker regions are disclosed. The back sides of the membranes can be rough compared with the smooth front sides. The membranes can be used in vitro to grow monolayers of cells in a laboratory or in vivo as surgically implantable growth layers, such as to replace the Bruch's membrane in the eye. The application further provides an implantable cage-like apparatus for culturing cells comprising the parylene membrane.

First claim

What is claimed is:

1. A method of manufacturing an implantable membrane, the method comprising:
depositing a film material on a first substrate surface to form a supporting film;
creating a plurality of perforations on the supporting film;
removing the supporting film from the first substrate surface;
attaching the supporting film to a second substrate surface;
depositing a parylene layer over the supporting film wherein the parylene layer is sufficient to cover a bottom of each perforation on the supporting film to form a membrane with a smooth first side;
orienting the membrane such that the smooth first side is positioned toward a cell culture; and
diffusing molecules through the membrane.

2. The method of manufacturing of claim 1 , wherein the first substrate surface is smooth.

3. The method of manufacturing of claim 1 , wherein etching is used to create the plurality of perforations.

4. The method of manufacturing of claim 1 , wherein lithography is used to create the plurality of perforations.

5. The method of manufacturing of claim 1 , wherein the plurality of perforations extend from a first side of the first supporting film to an opposing, second side of the supporting film.

6. The method of manufacturing of claim 1 , wherein the second substrate surface is smooth.

7. The method of manufacturing of claim 1 , wherein the parylene layer has a thickness between 0.01 μm to 5 μm.

8. The method of manufacturing of claim 1 , wherein the orientation of the membrane is configured to reduce adherence of cells on the smooth side of the membrane.

9. The method of manufacturing of claim 1 , further comprising the mechanical strength of the membrane by attaching a cage-like structure or mesh.

10. A method of manufacturing a structure for providing mechanical strength to an implantable membrane, the method comprising
depositing a first layer of parylene-C on silicone;
depositing a first material on the first layer of parylene-C;
applying a second coating over the first material;
lithographing and wet-etching the first material and the second coating to form a mask for parylene-C etching;
etching the first layer of parylene-C to form a mesh frame;
depositing a second layer of parylene-C on the mesh frame;
lithographing the mesh frame and depositing a layer of photo-resist on the mesh frame;
etching away undesirable regions in the mesh frame; and
removing the mesh frame from the silicone.

11. The method of manufacturing of claim 10 , wherein the first layer of parylene C has a thickness of about 1 μm to 10 μm.

12. The method of manufacturing of claim 10 , wherein the first layer of parylene-C has a thickness of about 6 μm.

13. The method of manufacturing of claim 10 , wherein the deposition of the first layer of parylene-C is performed with a vaporizer at a temperature 180° C.

14. The method of manufacturing of claim 10 , wherein the deposition of the first layer of parylene-C is performed with a furnace at a temperature of 690° C.

15. The method of manufacturing of claim 10 , wherein the deposition of the first layer of parylene-C is performed at a chamber pressure of 35 mTorr.

16. The method of manufacturing of claim 10 , wherein the silicone is treated with hexamethyldisilazane.

17. The method of manufacturing of claim 10 , wherein the first material is aluminum.

18. The method of manufacturing of claim 10 , wherein the second coating is a photoresist spin-coating.

19. The method of manufacturing of claim 10 , wherein the etching of the first layer of parylene-C is performed using reactive ion etching.

20. The method of manufacturing of claim 19 , further comprising the step of applying hydrofluoric acid to clean residue from the inside of the plurality of circular through holes, wherein the mesh frame comprises a plurality of circular through holes and the second layer of parylene-C has a thickness between 0.15 μm and 0.80 μm.

Assignees

• California Inst Of Techn
• Univ Southern California

Inventors

• Tai Yu-Chong
• Lu Bo
• Humayun Mark

Classifications

• C12N5/0068 Primary

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title

• A61L2430/16

  for reconstruction of eye parts, e.g. intraocular lens, cornea · CPC title

• A61L27/18

  obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds · CPC title

• C12N2533/30

  Synthetic polymers · CPC title

• A61F2/02

  Prostheses implantable into the body · CPC title