Cell and biofactor printable biopapers

Patent metadata

Field	Value
Publication number	US-10167464-B2
Application number	US-201414177240-A
Country	US
Kind code	B2
Filing date	Feb 11, 2014
Priority date	Apr 29, 2010
Publication date	Jan 1, 2019
Grant date	Jan 1, 2019

Abstract

Disclosed herein is a structure having: a porous polymeric film permeated by a first extracellular matrix material; and a topcoat layer comprising a second extracellular matrix gel disposed on the film. Also disclosed herein is a method of: providing a porous polymeric film; permeating the film with a first extracellular matrix material; and applying a topcoat layer of a second extracellular matrix material to the film. Also disclosed herein is a method of: laser-machining holes through a film comprising collagen to form a web-like structure.

First claim

What is claimed is:

1. A method comprising:
providing a plurality of structures each comprising:
a porous polymeric film having pores permeated by a first extracellular matrix material; and
a topcoat layer comprising a second extracellular matrix gel disposed on the film;

placing living cells on or within each topcoat layer to form cell-seeded structures; and
stacking the cell-seeded structures to form a stacked structure having alternating layers of polymeric film and topcoat layers;
wherein at least one of the cell-seeded structures has cells positioned in a branched pattern before the cell-seeded structures are stacked.

2. The method of claim 1 , wherein the cells are placed by a laser transfer method, laser-based printing, ink jet printing, micropen printing, syringe deposition, electrospray deposition, or a conformal printing method.

3. The method of claim 1 , further comprising:
incubating the stacked structure under conditions to induce cell differentiation or cell growth.

4. The method of claim 3 , wherein the first or second extracellular matrix material comprises a growth factor.

5. The method of claim 1 , wherein the living cells are placed in the branched pattern.

6. The method of claim 1 , wherein the living cells are placed in a branched pattern comprising more than one type of cell.

7. The method of claim 1 , wherein providing the structure comprises:
providing the porous polymeric film; and
permeating the film with the first extracellular matrix material;
wherein the film is not cell-seeded.

8. The method of claim 1 , wherein the branched pattern is formed by incubating the cell-seeded structures before stacking.

9. The method of claim 1 , wherein the stacked structure comprises a vascular network that allows for flow of materials into and out of the stacked structure.

Assignees

• Ringeisen Bradley R
• Pirlo Russell Kirk
• Wu Peter K
• Us Navy

Inventors

• Ringeisen Bradley R
• Pirlo Russell Kirk
• Wu Peter K

Classifications

• C12N5/0068 Primary

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title

• Y10T428/24273

  including aperture · CPC title

• C09D7/63

  organic · CPC title

• C12N11/08

  the carrier being a synthetic polymer · CPC title

• C12N11/02 Primary

  Enzymes or microbial cells immobilised on or in an organic carrier · CPC title