Systems, methods, and workflows for optogenetics analysis

Patent metadata

Field	Value
Publication number	US-10161937-B2
Application number	US-201715645426-A
Country	US
Kind code	B2
Filing date	Jul 10, 2017
Priority date	Aug 23, 2010
Publication date	Dec 25, 2018
Grant date	Dec 25, 2018

Abstract

The invention provides methods for characterizing cellular physiology by incorporating into an electrically excitable cell an optical reporter of, and an optical actuator of, electrical activity. A signal is obtained from the optical reporter in response to a stimulation of the cell. Either or both of the optical reporter and actuator may be based on genetically-encoded rhodopsins incorporated into the cell. The invention provides all optical methods that may be used instead of, or as a complement to, traditional patch clamp technologies and that can provide rapid, accurate, and flexible assays of cellular physiology.

First claim

What is claimed is:

1. A method for measuring network effects, the method comprising:
using an optical actuator of electrical activity incorporated into a first plurality of cells of one cell type;
obtaining a signal using an optical reporter of electrical activity incorporated into a second plurality of cells of another cell type; and
evaluating the signal.

2. The method of claim 1 , wherein the first plurality of cells of one cell type further comprises an optical reporter of electrical activity.

3. The method of claim 2 , wherein the second plurality of cells of one cell type further comprises an optical actuator of electrical activity.

4. The method of claim 1 , wherein the first plurality of cells comprises a first class of neurons.

5. The method of claim 1 , wherein the second plurality of cells comprises a second class of neurons.

6. The method of claim 1 , wherein the first plurality of cells comprises a first class of cardiomyocytes.

7. The method of claim 1 , wherein the second plurality of cells comprises a second class of cardiomyocytes.

8. The method of claim 1 , wherein the first plurality of cells comprises a first class of cells of iPSC's.

Assignees

• Harvard College

Inventors

• Cohen Adam Ezra
• Kralj Joel
• Douglass Adam D
• Hochbaum Daniel

Classifications

• A61K49/0017

  Fluorescence in vivo · CPC title

• G01N33/566 Primary

  using specific carrier or receptor proteins as ligand binding reagents {where possible specific carrier or receptor proteins are classified with their target compounds} · CPC title

• G01N2333/705

  Assays involving receptors, cell surface antigens or cell surface determinants · CPC title

• A61K49/0097

  Cells, viruses, ghosts, red blood cells, viral vectors, used for imaging or diagnosis in vivo · CPC title

• G01N33/54373

  involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings · CPC title