Method for harvesting stem and progenitor cells and method of treatment of kidney disease and neurological disease

Patent metadata

Field	Value
Publication number	US-10160955-B2
Application number	US-201615178746-A
Country	US
Kind code	B2
Filing date	Jun 10, 2016
Priority date	Jun 30, 2015
Publication date	Dec 25, 2018
Grant date	Dec 25, 2018

Abstract

A method of generating one of podocyte progenitor cells and neural stem/progenitor cells comprising the steps of collecting a urine specimen from a patient; centrifuging the urine specimen; and removing the one of podocyte progenitor cells and neural stem/progenitor cells.

First claim

Wherefore, I claim:

1. A method of treating a central nervous system disease condition in a patient comprising the steps of:
isolating neural stem/progenitor cells from a urine specimen of the patient;
growing the neural stem/progenitor cells in vitro;
selecting cells that have positive expression for CD133; and
implanting the neural selected stem/progenitor cells into the patient.

2. The method of claim 1 wherein the central nervous system disease condition is Parkinson's disease.

3. The method of claim 1 wherein the central nervous system disease condition is Huntington's disease.

4. The method of claim 1 wherein the central nervous system disease condition is and multiple sclerosis.

5. The method of claim 1 wherein the neural stem/progenitor cells are implanted in the patient via intracerebral transplantation.

6. The method of claim 1 wherein the neural stem/progenitor cells are implanted in the patient via xenotransplantation.

7. The method of claim 1 further comprising the step of selecting cells that have positive expression for one of nestin and SOX2.

8. The method of claim 7 wherein the selected cells also have positive expression for podocin.

9. The method of claim 1 , wherein the neural stem/progenitor cells are implanted into a central nervous system of the patient.

10. The method of claim 1 further comprising the step of selecting cells that have positive expression for each of nestin, SOX2, and podocin.

11. The method of claim 1 further comprising the step of selecting cells that have positive expression for Oct-4.

12. The method of claim 1 wherein the neural stem/progenitor cells are grown in vitro in a medium including insulin, transferrin, and selenium.

13. The method of claim 1 wherein the neural stem/progenitor cells are grown in vitro in a medium including RPMI medium 1640.

14. The method of claim 1 wherein the neural stem progenitor cells are grown in vitro in a medium in the absence of growth factors.

15. The method of claim 1 wherein the neural stem/progenitor cells are grown in vitro in a medium in the absence of one of human epidermal growth factor (hEGF), platelet-derived growth factor (PDGF), transforming growth factor-(TGF-), and basic fibroblast growth factor (bFGF).

16. The method of claim 1 wherein the neural stem/progenitor cells are grown in vitro in a medium in the absence of each of human epidermal growth factor (hEGF), platelet-derived growth factor (PDGF), transforming growth factor-(TGF-), and basic fibroblast growth factor (bFGF).

17. A method of treating a central nervous system disease condition in a patient comprising the steps of:
isolating neural stem/progenitor cells from a urine specimen of the patient;
growing the neural stem/progenitor cells in vitro;
selecting cells that have positive expression for podocin; and
implanting the selected neural stem/progenitor cells into the patient.

18. The method of claim 17 wherein the neural stem/progenitor cells are grown in vitro in a medium including insulin, transferrin, and selenium.

19. The method of claim 18 wherein the medium further includes RPMI medium 1640 and the selected cells also have positive expression for CD133, Oct-4, nestin, and SOX2.

Assignees

• Univ Louisiana State
• Board Of Supervisors Of Louisiana State Univ

Inventors

• Wang Yuping

Classifications

• C12N5/0623

  Stem cells · CPC title

• A61K35/30

  Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue · CPC title

• A61K35/22

  Urine; Urinary tract, e.g. kidney or bladder; Intraglomerular mesangial cells; Renal mesenchymal cells; Adrenal gland · CPC title

• C12N5/0687 Primary

  Renal stem cells; Renal progenitors · CPC title