Method for differentiation of pluripotent stem cells into multi-competent renal precursors

Patent metadata

Field	Value
Publication number	US-10138468-B2
Application number	US-201615010389-A
Country	US
Kind code	B2
Filing date	Jan 29, 2016
Priority date	Jul 29, 2013
Publication date	Nov 27, 2018
Grant date	Nov 27, 2018

Abstract

This application relates to a method for differentiating pluripotent stem cells (PSCs) into multi-competent renal precursor cells expressing Six2. These renal precursor cells are able to differentiate into fully functional and fully differentiated podocytes. Moreover this application relates to a method for differentiating human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) into defined renal precursor cells expressing Six2 and podocytes based on linked steps of chemically defined medium inductions.

First claim

The invention claimed is:

1. A method for differentiating human or mouse pluripotent stem cells into renal precursor cells expressing SIX2, WT1 and SALL1, the method comprising the steps of:
a) providing a monolayer of human or mouse pluripotent stem cells in a pluripotency medium,
b) incubating the cells of step a) in an adherent culture for at least three days in a medium supplemented with 3-(3-amino-phenyl)-4-(1-methyl-1H-indol-3-yl)-pyrrole-2,5-dione and recombinant bone morphogenic protein-4 (BMP4), and
c) differentiating the cells of step b) in a serum-free medium supplemented with recombinant bone morphogenic protein-7 (BMP7) and retinoic acid, to produce renal precursor cells expressing SIX2, WT1 and SALL1.

2. The method of claim 1 , wherein the pluripotency medium of step a) is a serum-free medium supplemented with an inhibitor of the Rho-associated coiled-coil forming protein serine/threonine kinase family of protein kinases (ROCK kinase inhibitor).

3. The method of claim 2 , wherein the ROCK kinase inhibitor is selected from the group consisting of 1-(5-Isoquinolinesulfonyl) homopiperazine, N-Benzyl-2-(pyrimidin-4-ylamino) thiazole-4-carboxamide, and (+)-(R)-trans-4-(1-aminoethyl)-N-(4-pyridyl) cyclohexanecarboxamide dihydrochloride).

4. The method of claim 1 , wherein the medium of step b) is a serum free medium supplemented with insulin, transferrin and progesterone.

5. The method of claim 1 , wherein step a) comprises incubating the cells in the pluripotency medium for 18 hours to 30 hours.

6. The method of claim 1 , wherein step b) comprises incubating the cells in the medium for 3 to 4 days.

7. The method of claim 1 , wherein step c) comprises incubating the cells in the medium for 18 hours to 48 hours.

8. The method of claim 1 , wherein the pluripotent stem cell is an induced pluripotent stem cell.

9. The method claim 8 , wherein the induced pluripotent stem cell is obtained from a subject suffering from a renal disease.

10. A method for differentiating human or mouse pluripotent stem cells into renal precursor cells expressing SIX2, WT1 and SALL1, the method comprising the steps of:
a) providing a monolayer of human or mouse pluripotent stem cells in a pluripotency medium,
b) incubating the cells of step a) in an adherent culture for at least three days in a medium supplemented with 3-(3-amino-phenyl)-4-(1-methyl-1H-indo-1-3-yl)-pyrrole-2,5-dione and recombinant bone morphogenic protein-4 (BMP4),
c) differentiating the cells of step b) in a serum-free medium supplemented with recombinant bone morphogenic protein-7 (BMP7) and retinoic acid, to produce renal precursor cells expressing SIX2, WT1 and SALL1, and
d) incubating the renal precursor cells of step c) in a medium comprising retinoic acid and vitamin D3 to obtain proliferating podocytes.

11. The method of claim 10 , wherein the medium of step d) is supplemented with 10-200 nM vitamin D3.

Assignees

• Hoffmann La Roche

Inventors

• Bailly Jacques
• Ciampi Osele
• Graf Martin
• Iacone Roberto
• Patsch Christoph

Classifications

• C12N2501/727

  Kinases (EC 2.7.) · CPC title

• C12N2506/45

  from artificially induced pluripotent stem cells · CPC title

• C12N2501/155

  Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor · CPC title

• C12N2501/385

  of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR] · CPC title

• C12N2500/25

  Insulin-transferrin; Insulin-transferrin-selenium · CPC title