Cell culture device and manufacturing method therof

Patent metadata

Field	Value
Publication number	US-10150945-B2
Application number	US-18762105-A
Country	US
Kind code	B2
Filing date	Jul 21, 2005
Priority date	Jul 23, 2004
Publication date	Dec 11, 2018
Grant date	Dec 11, 2018

Abstract

A low-cost cell culture device with low cytotoxicity and improved cell adhesion is described, manufactured by a simplified coating process. At least the portion for cell culture on the surface of the cell culture device is coated with at least one of ε-poly-L-lysine, further polymerized derivatives of ε-poly-L-lysine and their salts.

First claim

What is claimed is:

1. A cell culture device wherein the surface of substrate is coated with at least one of further polymerized derivatives of ε-poly-L-lysine and salts of further polymerized derivatives of ε-poly-L-lysine, wherein the further polymerized derivatives of ε-poly-L-lysine are derived from dehydration-condensation of ε-poly-L-lysine,
wherein the amount coated of at least one of further polymerized derivatives of ε-poly-L-lysine and salts of further polymerized derivatives of ε-poly-L-lysine is in a range 10 ng/cm 2 to 140 ng/cm 2 .

2. The device of claim 1 , wherein the number average molecular weight of the further polymerized derivatives of ε-poly-L-lysine is in a range 8,000 to 100,000.

3. The device of claim 1 , wherein the substrate is glass.

4. The device of claim 1 , wherein the substrate is synthetic resin.

5. A method of manufacturing a cell culture device wherein the surface of substrate is coated with at least one of further polymerized derivatives of ε-poly-L-lysine and salts of further polymerized derivatives of ε-poly-L-lysine, including a step wherein the substrate of the device is brought into contact with a solution of at least one of further polymerized derivatives of ε-poly-L-lysine and salts of further polymerized derivatives of ε-poly-L-lysine, wherein the further polymerized derivatives of ε-poly-L-lysine are derived from dehydration-condensation of ε-poly-L-lysine,
wherein the amount coated of at least one of further polymerized derivatives of ε-poly-L-lysine and salts of further polymerized derivatives of ε-poly-L-lysine is in a range 10 ng/cm 2 to 140 ng/cm 2 .

6. The method of claim 5 , wherein the number average molecular weight of the further polymerized derivatives of ε-poly-L-lysine is in a range 8,000 to 100,000.

7. The method of claim 5 , including a step of drying the surface after the contact with a solution of at least one of further polymerized derivatives of ε-poly-L-lysine and salts of further polymerized derivatives of ε-poly-L-lysine.

8. The method of claim 5 , including a step of removing an excessive amount of the solution after the contact with a solution of at least one of further polymerized derivatives of ε-poly-L-lysine and salts of further polymerized derivatives of ε-poly-L-lysine.

9. The method of claim 5 , including a step of removing an excessive amount of the solution followed by a step of drying the surface, after the contact with a solution of at least one of further polymerized derivatives of ε-poly-L-lysine and salts of further polymerized derivatives of ε-poly-L-lysine.

10. The method of claim 5 , wherein a solvent of the solution of at least one of further polymerized derivatives of ε-poly-L-lysine and salts of further polymerized derivatives of ε-poly-L-lysine is a borate buffer solution.

11. The method of claim 5 , wherein the substrate of the device is glass.

12. The method of claim 5 , wherein the substrate of the device is synthetic resin.

Assignees

• Nagasaki Takeshi
• Shinkai Seiji
• Uno Atsushi
• Jnc Corp
• Takeshi Nagasaki

Inventors

• Nagasaki Takeshi
• Shinkai Seiji
• Uno Atsushi

Classifications

• C12N5/0068 Primary

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title

• C12N2533/12

  Glass · CPC title

• C12N2533/32

  Polylysine, polyornithine · CPC title

• C12M3/10

  for culture in eggs · CPC title

• C12M1/22 Primary

  Petri dishes · CPC title