Microorganism including gene encoding protein having dehalogenase activity and method of reducing concentration of fluorinated methane in sample using the same

Patent metadata

Field	Value
Publication number	US-10150080-B2
Application number	US-201615332525-A
Country	US
Kind code	B2
Filing date	Oct 24, 2016
Priority date	Oct 23, 2015
Publication date	Dec 11, 2018
Grant date	Dec 11, 2018

Abstract

Provided is a microorganism including a gene encoding a protein having a dehalogenase activity, a composition for using in reducing a concentration of fluorinated methane in a sample, the composition including the microorganism including the gene encoding the protein having the dehalogenase activity, and a method of reducing the concentration of fluorinated methane in the sample.

First claim

What is claimed is:

1. A recombinant microorganism comprising a nucleic acid encoding chloroform reductive dehalogenase CfrA from the genus Dehalobacter , wherein the recombinant microorganism belongs to the genus Xanthobacter, Agrobacterium, Corynebacterium, Rhodococcus, Mycobacterium, Klebsiella , or Escherichia , and the recombinant microorganism has increased dehalogenase activity compared to a parent strain of the recombinant microorganism.

2. The recombinant microorganism of claim 1 , wherein the microorganism comprises one or more nucleic acids comprising a promoter operably linked to a nucleic acid sequence encoding the CfrA, wherein one or more of the nucleic acids is heterologous to the microorganism.

3. The recombinant microorganism of claim 1 , wherein the nucleic acid sequence encoding the CfrA comprises SEQ ID NO: 5.

4. The recombinant microorganism of claim 1 , wherein the recombinant microorganism reduces a concentration of fluorinated methane in a sample contacted with the recombinant microorganism, wherein fluorinated methane is represented by CH n F 4-n , where n is an integer of 0 to 3.

5. The recombinant microorganism of claim 4 , wherein reducing the concentration of fluorinated methane comprises cleaving C—F bonds of the fluorinated methane, converting the fluorinated methane into other materials, or intracellular accumulation of the fluorinated methane.

6. The recombinant microorganism of claim 4 , wherein the fluorinated methane is CF 4 , CHF 3 , or CH 2 F 2 .

7. A method of reducing the concentration of fluorinated methane in a sample, the method comprising contacting a recombinant microorganism of claim 1 with a sample containing fluorinated methane represented by CH n F 4-n , where n is an integer of 0 to 3, to reduce the concentration of fluorinated methane in the sample.

8. The method of claim 7 , wherein the recombinant microorganism is contacted with the sample in an air-sealed container.

9. The method of claim 7 , wherein contacting the recombinant microorganism with the sample comprises culturing or incubating the recombinant microorganism with the sample.

10. The method of claim 7 , wherein the recombinant microorganism proliferates in the air-sealed container.

11. The method of claim 7 , wherein the fluorinated methane is CF 4 , CHF 3 , or CH 2 F 2 .

12. The method of claim 7 , wherein the microorganism belongs to the genus Escherichia.

13. A method of preparing a recombinant microorganism of claim 1 , the method comprising introducing into a microorganism a nucleic acid encoding chloroform reductive dehalogenase CfrA from the genus Dehalobacter , wherein the microorganism belongs to the genus Xanthobacter, Agrobacterium, Corynebacterium, Rhodococcus, Mycobacterium, Klebsiella , or Escherichia.

Assignees

• Samsung Electronics Co Ltd

Inventors

• Chu Hunsu
• Park Joonsong
• Park Jinhwan

Classifications

• B01D2255/804

  Enzymatic · CPC title

• C12N9/14

  Hydrolases (3) · CPC title

• B01D2251/95

  Specific microorganisms · CPC title

• C12Y308/01002

  (S)-2-Haloacid dehalogenase (3.8.1.2) · CPC title

• C12Y308/01005

  Haloalkane dehalogenase (3.8.1.5) · CPC title