Microorganism including gene encoding protein having hydroxylase activity and method of reducing concentration of fluorinated methane in sample using the same

Patent metadata

Field	Value
Publication number	US-2016333359-A1
Application number	US-201615154575-A
Country	US
Kind code	A1
Filing date	May 13, 2016
Priority date	May 13, 2015
Publication date	Nov 17, 2016
Grant date	—

Abstract

A microorganism including a foreign gene encoding a protein having a hydroxylase activity that reduces the concentration of CH n F 4-n (n is an integer of 0 to 3) in a sample, as well as a composition including the microorganism or lysate thereof, and a method of reducing the concentration of CH n F 4-n in a sample using the microorganism or lysate.

First claim

What is claimed is:

1 . A composition for using in reducing CH n F 4-n concentration in a sample, where n is an integer of 0 to 3, the composition comprising:
a recombinant microorganism wherein the recombinant microorganism comprises one or more foreign genes encoding a protein having a hydroxylase activity, and has an increased hydroxylase activity compared to a parent strain of the recombinant microorganism.

2 . The composition of claim 1 , wherein the protein having a hydroxylase activity acts on carbon-fluorine or carbon-hydrogen bond of a fluoroalkane compound.

3 . The composition of claim 1 , wherein the protein having a hydroxylase activity is selected from the group consisting of dehalogenase and monooxygenase.

4 . The composition of claim 3 , wherein the dehalogenase is tetrachloroethene reductive dehalogenase, dichloromethane dehalogenase, haloalkane dehalogenase, alkylhalidase, (S)-2-haloacid dehalogenase, (R)-2-haloacid dehalogenase, 2-haloacid dehalogenase (configuration-inverting), haloacetate dehalogenase, or a combination thereof.

5 . The composition of claim 3 , wherein the monooxygenase is soluble methane monooxygenase (sMMO), ammonia monooxygenase, camphor 5-monooxygenase, cytochrome P450, or a combination thereof.

6 . The composition of claim 5 , wherein the cytochrome P450 is bacterial cytochrome P450.

7 . The composition of claim 1 , wherein the protein is one or more selected from the group consisting of haloalkane dehalogenase (dhlA) from Xanthobacter autotrophicus , (S)-2-haloacid dehalogenase (dhlB) from Xanthobacter autotrophicus , P450 CAM from Pseudomonas putida , P450 BM3 from Bacillus megaterium , and soluble methane monooxygenase (sMMO) from Methylococcus capsulatus.

8 . The composition of claim 1 , wherein the microorganism belongs to the genus Escherichia or xanthobacter.

9 . A method of reducing a concentration of CH n F 4-n in a sample, where n is an integer of 0 to 3, the method comprising:
contacting a recombinant microorganism with a sample containing CH n F 4-n , where n is an integer of 0 to 3, to reduce the concentration of CH n F 4-n in the sample, wherein the recombinant microorganism comprises one or more foreign genes encoding a protein having a hydroxylase activity, and the recombinant microorganism has an increased hydroxylase activity, compared to a parent strain of the recombinant microorganism.

10 . The method of claim 9 , wherein the protein is selected from the group consisting of dehalogenase and monooxygenase.

11 . The method of claim 10 , wherein the dehalogenase is tetrachloroethene reductive dehalogenase, dichloromethane dehalogenase, haloalkane dehalogenase, alkylhalidase, (S)-2-haloacid dehalogenase, (R)-2-haloacid dehalogenase, 2-haloacid dehalogenase (configuration-inverting), haloacetate dehalogenase, or a combination thereof.

12 . The method of claim 10 , wherein the monooxygenase is soluble methane monooxygenase (sMMO), ammonia monooxygenase, camphor 5-monooxygenase, cytochrome P450, or a combination thereof.

13 . The method of claim 12 , wherein the cytochrome P450 is bacterial cytochrome P450.

14 . The method of claim 9 , wherein the protein is one or more selected from the group consisting of haloalkane dehalogenase (dhlA) from Xanthobacter autotrophicus , (S)-2-haloacid dehalogenase (dhlB) from Xanthobacter autotrophicus , P450 CAM from Pseudomonas putida , P450 BM3 from Bacillus megaterium , and soluble methane monooxygenase (sMMO) from Methylococcus capsulatus.

15 . The method of claim 9 , wherein the contacting is performed in a sealed container.

16 . The method of claim 9 , wherein the contacting comprises culturing or incubating the recombinant microorganism with the sample containing CH n F 4-n where n is an integer of 0 to 3.

17 . The method of claim 9 , wherein the contacting comprises culturing the recombinant microorganism in a sealed container under conditions where the recombinant microorganism proliferates.

18 . The method of claim 9 , wherein the microorganism belongs to the genus Escherichia or xanthobacter.

19 . The method of claim 9 , wherein reducing the concentration of CH n F 4-n in the sample comprises converting of CH n F 4-n into another material, optionally be cleaving one or more C—F bonds of CH n F 4-n ; or increasing intracellular accumulation of CH n F 4-n .

20 . The method of claim 9 , wherein the sample is in a liquid or gas state.

Assignees

• Samsung Electronics Co Ltd

Inventors

• Song Seunghoon
• Kim Taeyong
• Park Jinhwan
• Park Joonsong
• Jung Yukyung
• Chu Hunsu
• Song Jiyoon
• Cho Kwangmyung

Classifications

• C12Y114/99039

  Ammonia monooxygenase (1.14.99.39) · CPC title

• C12Y114/00

  Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) · CPC title

• C12N9/0077

  with a reduced iron-sulfur protein as one donor (1.14.15) · CPC title

• C12Y114/13025

  Methane monooxygenase (1.14.13.25) · CPC title

• B01D53/70

  Organic halogen compounds · CPC title