Polypeptides having protease activity and polynucleotides encoding same

Patent metadata

Field	Value
Publication number	US-10131864-B2
Application number	US-201414784488-A
Country	US
Kind code	B2
Filing date	Apr 11, 2014
Priority date	Apr 18, 2013
Publication date	Nov 20, 2018
Grant date	Nov 20, 2018

Abstract

The present invention relates to isolated polypeptides having protease activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of using the polypeptides in beer production.

First claim

The invention claimed is:

1. A nucleic acid construct or expression vector comprising a polynucleotide encoding a polypeptide having protease activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide in an expression host, and wherein the polypeptide having protease activity comprises an amino acid sequence having at least 85% sequence identity to the amino acid sequence of SEQ ID NO: 1.

2. An isolated recombinant host cell transformed with the nucleic acid construct or expression vector of claim 1 .

3. A method of producing a polypeptide having protease activity, comprising cultivating the isolated recombinant host cell of claim 2 , under conditions conducive for production of the polypeptide.

4. The nucleic acid construct or expression vector of claim 1 , wherein the polypeptide having protease activity comprises an amino acid sequence that has at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 1.

5. The nucleic acid construct or expression vector of claim 1 , wherein the polypeptide having protease activity comprises an amino acid sequence that has at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 1.

6. The nucleic acid construct or expression vector of claim 1 , wherein the polypeptide having protease activity comprises an amino acid sequence that has at least 99% sequence identity to the amino acid sequence of SEQ ID NO: 1.

7. The nucleic acid construct or expression vector of claim 1 , wherein the polypeptide having protease activity is encoded by a polynucleotide comprising a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence of SEQ ID NO: 2.

8. The nucleic acid construct or expression vector of claim 1 , wherein the polypeptide having protease activity is encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence of SEQ ID NO: 2.

9. The nucleic acid construct or expression vector of claim 1 , wherein the polypeptide having protease activity is encoded by a polynucleotide comprising a nucleotide sequence having at least 99% sequence identity to the nucleotide sequence of SEQ ID NO: 2.

10. The nucleic acid construct or expression vector of claim 1 , wherein the polypeptide having protease activity comprises the amino acid sequence of SEQ ID NO: 1.

11. An isolated recombinant host cell transformed with the nucleic acid construct or expression vector of claim 4 .

12. An isolated recombinant host cell transformed with the nucleic acid construct or expression vector of claim 5 .

13. An isolated recombinant host cell transformed with the nucleic acid construct or expression vector of claim 6 .

14. An isolated recombinant host cell transformed with the nucleic acid construct or expression vector of claim 10 .

15. A method of producing a polypeptide having protease activity, comprising cultivating the isolated recombinant host cell of claim 11 , under conditions conducive for production of the polypeptide.

16. A method of producing a polypeptide having protease activity, comprising cultivating the isolated recombinant host cell of claim 12 , under conditions conducive for production of the polypeptide.

17. A method of producing a polypeptide having protease activity, comprising cultivating the isolated recombinant host cell of claim 13 , under conditions conducive for production of the polypeptide.

18. A method of producing a polypeptide having protease activity, comprising cultivating the isolated recombinant host cell of claim 14 , under conditions conducive for production of the polypeptide.

Assignees

• Novozymes As
• Novozyme As

Inventors

• Borchert Martin Simon
• Gjermansen Morten
• Oestergaard Peter Rahbek
• Baekgaard Lone
• Mauch Alexander
• Heldt-Hansen Hans Peter
• Bhatia Anne Mette
• Lund Christina
• Toscano Miguel Duarte Guilherme Pereira

Classifications

• C12N9/60

  from yeast · CPC title

• C12C11/003

  Fermentation of beerwort · CPC title

• C12C5/004 Primary

  Enzymes · CPC title

• C12Y304/24

  Metalloendopeptidases (3.4.24) · CPC title

• C12N9/52

  derived from bacteria {or Archaea} · CPC title