Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides

Patent metadata

Field	Value
Publication number	US-10119944-B2
Application number	US-201414576682-A
Country	US
Kind code	B2
Filing date	Dec 19, 2014
Priority date	Dec 24, 2013
Publication date	Nov 6, 2018
Grant date	Nov 6, 2018

Abstract

The invention relates to poly-amide bonded hydrophilic interaction chromatography (HILIC) stationary phases and novel HILIC methods for use in the characterization of large biological molecules modified with polar groups, known to those skilled in the art as glycans. The invention particularly provides novel, poly-amide bonded materials designed for efficient separation of large biomolecules, e.g. materials having a large percentage of larger pores (i.e. wide pores). Furthermore, the invention advantageously provides novel HILIC methods that can be used in combination with the stationary phase materials described herein to effectively separate protein and peptide glycoforms by eliminating previously unsolved problems, such as on-column aggregation of protein samples, low sensitivity of chromatographic detection of the glycan moieties, and low resolution of peaks due to restricted pore diffusion and long intra/inter-particle diffusion distances.

First claim

What is claimed is:

1. A porous material comprising a copolymer comprising at least one hydrophilic monomer and a first poly-amide bonded phase and a second poly-amide bonded phase, wherein:
the average pore diameter is greater than or equal to about 200 Å;
the porous material comprises:
a porous or nonporous organic-inorganic hybrid core comprising an aliphatic bridged silane,
at least one hydrophilic monomer grafted to the core,
and the first and the second poly-amide bonded phase bonded to the at least one hydrophilic monomer and forming a chemically modified chromatographic surface wherein there is a distinct transition from the core to the chromatographic surface;

and wherein the second poly-amide bonded phase is derived from N,N-methylenebisacrylamide, N,N-ethylenebisacrylamide, N,N-propylenebisacrylamide, N,N-butylenebisacrylamide, N,N′-(1,2-dihydroxyethylene)bisacrylamide, or 1,4-bis(acryloyl)piperazine.

2. The porous material of claim 1 , wherein the porous material comprises a porous particle that comprises said copolymer.

3. The porous material of claim 1 , wherein the porous material comprises a porous monolith that comprises said copolymer.

4. The porous material of claim 1 , wherein said hydrophilic monomer is 3-methacryloxypropyltrichlorosilane, 3-methacryloxypropylmethyldichlorosilane, 3-methacryloxypropyldimethylchlorosilane, 3-methacryloxypropyltrimethoxysilane, 3-methacryloxypropylmethyldimethoxysilane, 3-methacryloxypropyldimethylmethoxysilane, 3-methacryloxypropyltriethoxysilane, 3-methacryloxypropylmethyldiethoxysilane, 3-methacryloxypropyldimethylethoxysilane, 3-acryloxypropyltrichlorosilane, 3-acryloxypropylmethyldichlorosilane, 3-acryloxypropyldimethylchlorosilane 3-acryloxypropyltrimethoxysilane, 3-acryloxypropylmethyldimethoxysilane, 3-acryloxypropyldimethylmethoxysilane, 3-acryloxypropyltriethoxysilane, 3-acryloxypropylmethyldiethoxysilane, 3-acryloxypropyldimethylethoxysilane, styrylethyltrichlorosilane, styrylethylmethyldichlorosilane, styrylethyldimethylchlorosilane, styrylethyltrimethoxysilane, styrylethylmethyldimethoxysilane, styrylethyldimethylmethoxysilane, styrylethyltriethoxysilane, styrylethylmethyldiethoxysilane, styrylethyldimethylethoxysilane, vinyltriethoxysilane, vinyltrimethoxysilane, N-(3-acryloxy-2-hydroxypropyl)-3-aminopropyl triethoxysilane, (3-acryloxypropyl) trimethoxysilane, O-(methacryloxyethyl)-N-(triethoxysilylpropyl)urethane, N-(3-methacryloxy-2-hydroxypropyl)-3-aminopropyl triethoxysilane, methacryloxy methyltriethoxysilane, methacryloxymethyl trimethoxysilane, methacryloxypropy methyldiethoxysilane, methacryloxypropyl methyldimethoxysilane, methacryl oxypropyltris (methoxyethoxy)silane, 3-(N-styrylmethyl-2-aminoethylamino) propyltrimethoxysilane hydrochloride,

5. The porous material of claim 1 , wherein said first poly-amide bonded phase is derived from acrylamide, divinylbenzene, styrene, ethylene glycol dimethacrylate, 1-vinyl-2-pyrrolidinone and tert-butylmethacrylate, methacrylamide, N,N′-(1,2-dihydroxyethylene)bisacrylamide, N,N′-ethylenebisacrylamide, N,N′-methylenebisacrylamide, butyl acrylate, ethyl acrylate, methyl acrylate, 2-(acryloxy)-2-hydroxypropyl methacrylate, N,N-bis(2-cyanoethyl)acrylamide, N-acryloyltris(hydroxymethyl)aminomethane, 3-(acryloxy)-2-hydroxypropyl methacrylate, trimethylolpropane triacrylate, trimethylolpropane ethoxylate triacrylate, tris[(2-acryloyloxy)ethyl]isocyanurate, acrylonitrile, methacrylonitrile, itaconic acid, methacrylic acid, trimethylsilylmethacrylate, N-[tris(hydroxymethyl)methyl]acrylamide, (3-acrylamidopropyl)trimethylammonium chloride, [3-(methacryloylamino)propyl]dimethyl(3-sulfopropyl)ammonium hydroxide inner salt,

6. The porous material of claim 1 , wherein the first poly-amide bonded phase is present in 35 to 99 mole % of the poly-amide bonded phases.

7. The porous material of claim 1 , wherein the second poly-amide bonded phase is present in 65 to 1 mole % of the poly-amide bonded phases.

8. The porous material of claim 1 , wherein the porous material has a median pore diameter of about 100 Å to about 1000 Å.

9. The porous material of claim 1 wherein the porous material has a nitrogen content of said material is from about 0.5% N to about 20% N as measured by combustion analysis.

10. A method for removing or isolating a component from a mixture comprising:
contacting the mixture with a chromatographic material comprising the porous material according to claim 1 , to thereby remove or isolate the component from the mixture.

11. A hydrophilic interaction chromatography cartridge comprising the porous material according to claim 1 .

Assignees

• Waters Technologies Corp

Inventors

• Lauber Matthew A
• Koza Stephan M
• Iraneta Pamela C
• Wyndham Kevin D

Classifications

• C08F230/08

  containing silicon · CPC title

• B01D15/305

  Hydrophilic interaction chromatography [HILIC] · CPC title

• B01J20/286

  Phases chemically bonded to a substrate, e.g. to silica or to polymers · CPC title

• G01N30/06 Primary

  Preparation · CPC title

• B01J20/3272

  Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds · CPC title