Bacterial/cellular recognition impedance algorithm

Patent metadata

Field	Value
Publication number	US-10094818-B2
Application number	US-99092109-A
Country	US
Kind code	B2
Filing date	May 7, 2009
Priority date	May 7, 2008
Publication date	Oct 9, 2018
Grant date	Oct 9, 2018

Abstract

A method for characterising cells or cell structures in a sample comprising: obtaining at least one normalized impedance response of the sample over at least one frequency range; and characterising at least one cell using at least one characteristic of the normalized impedance response.

First claim

The invention claimed is:

1. A method for characterizing unidentified cells or cell structures in a sample, said method comprising:
implanting at least a portion of a device within a subject containing the sample, the device comprising a controller and at least two electrodes;
monitoring, via the controller, at least one characteristic of the sample located in an electrical path between the electrodes;
obtaining, via the controller and utilizing the at least two electrodes, at least one normalized impedance response of the sample over at least one frequency range, wherein the normalized impedance is a measured impedance divided by a baseline impedance, wherein the measured impedance comprises at least one of a real or an imaginary part, and wherein the baseline impedance is an impedance response of the sample over the at least one frequency range with substantially no cells in an electrical path between at least two electrodes;
identifying, via the controller and within a set of data containing a plurality of normalized impedance responses associated with a corresponding plurality of cell types, at least one characteristic of the normalized impedance response over the at least one frequency range that is indicative of a single cell type within the plurality of cell types; and
characterizing, via the controller, at least one unidentified cell to determine cell identity by using the identified at least one characteristic of the normalized impedance response over the at least one frequency range,
wherein:
the determined cell identity is characteristic of at least a cell type and a stage of growth of the at least one unidentified cell in the sample; and
the at least one characteristic of the normalized impedance response used for characterizing the at least one unidentified cell is at least one of a number of peaks in the normalized impedance.

2. A method according to claim 1 , wherein the frequency range is a continuous or quasi-continuous frequency range or spectrum.

3. A method according to claim 1 , wherein the frequency range comprises a series of discrete frequency measurements.

4. A method according to claim 1 , wherein the frequency range is any frequency range lying between 0.1 Hz and 33 MHz.

5. A method according to claim 1 , wherein at least one of:
the at least one cell is a bacteria or other single cell organism;
the at least one cell is a plant or animal cell such as a plant or animal tissue cell;
at least one cell is arranged in a structure, such as plant or animal tissue or a multi-cell organism.

6. A method according to claim 1 , wherein the baseline impedance response is at least one of an initial or a calculated or estimated or standard impedance response of the system.

7. A method according to claim 6 , wherein the baseline impedance response of the system is further based at least in part upon measurement of an impedance response of at least one of a similar or a standardised system over the at least one frequency range with substantially no cells in an electrical path between at least two electrodes.

8. A method according to claim 1 , wherein the at least one measured impedance response of the system over the at least one frequency range is obtained after at least one of introduction or growth of at least one cell in the electrical path between at least two electrodes.

9. A method according to claim 1 , wherein the at least one characteristic of the normalized impedance response is further at least one of the frequency, the peak size, or the peak shape of at least one peak in the normalized impedance response over the frequency range.

10. A method according to claim 9 , wherein at least the at least one peak is used to determine the stage of growth of at least one cell or bacterial system.

11. A method according to claim 10 , wherein changes in at least one peak in the normalized system are tracked over time to allow the distinction of changes in cell or bacterial growth.

12. A method according to claim 9 , wherein the at least one peak in normalized impedance with frequency is characteristic of at least one of cell or bacterial type or growth stage and the normalized impedance is used to determine the presence of a particular cell, bacteria, tissue type, or molecular by-product in the system.

13. A method according to claim 1 , wherein at least two electrodes used to obtain the normalized impedance response are immersed in a culture medium.

14. A method according to claim 13 , wherein the culture medium is arranged to promote the growth of at least one type of cells.

15. A method according to claim 1 , wherein the measurement of at least one of the impedance response of the system, the baseline impedance response of the system, or the normalized Impedance response of the system is made using AC impedance techniques.

16. The method according to claim 1 , further comprising the steps of applying an electrical stimulus between the at least two electrodes and measuring a magnitude and phase of a current and a voltage between at least two points in the electrical path between the electrodes.

17. A method for characterizing unidentified cells or cell structures in a sample, said method comprising:
positioning a device within a dressing positioned adjacent a surface of a wound on a subject containing the sample, the device comprising a controller and at least two electrodes;
monitoring, via the controller, at least one characteristic of the sample located in an electrical path between the electrodes;
obtaining, via the controller and utilizing the at least two electrodes, at least one normalized impedance response of the sample over at least one frequency range, wherein the normalized impedance is a measured impedance divided by a baseline impedance, wherein the measured impedance comprises at least one of a real or an imaginary part, and wherein the baseline impedance is an impedance response of the sample over the at least one frequency range with substantially no cells in an electrical path between at least two electrodes;
identifying, via the controller and within a set of data containing a plurality of normalized impedance responses associated with a corresponding plurality of cell types, at least one characteristic of the normalized impedance response over the at least one frequency range that is indicative of a single cell type within the plurality of cell types; and
characterizing, via the controller, at least one unidentified cell to determine cell identity by using the identified at least one characteristic of the normalized impedance response over the at least one frequency range,
wherein:
the determined cell identity is characteristic of at least a cell type and a stage of growth of the at least one unidentified cell in the sample; and
the at least one characteristic of the normalized impedance response used for characterizing the at least one unidentified cell is at least one of a number of peaks in the normalized impedance.

18. The method according to claim 17 , wherein the frequency range is a continuous or quasi-continuous frequency range or spectrum.

19. The method according to claim 17 , wherein the frequency range comprises a series of discrete frequency measurements.

20. The method according to claim 17 , wherein the baseline impedance response is at least one of an initial or a calculated or estimated or standard impedance response of the system.

21. The method according to claim 20 , wherein the baseline impedance response of the system is further

Assignees

• Connolly Patricia
• Shedden Laurie
• Univ Strathclyde

Inventors

• Connolly Patricia
• Shedden Laurie

Classifications

• A61B5/445

  Evaluating skin irritation or skin trauma, e.g. rash, eczema, wound, bed sore · CPC title

• A61B5/1473

  invasive, e.g. introduced into the body by a catheter · CPC title

• G01N33/48735 Primary

  Investigating suspensions of cells, e.g. measuring microbe concentration (by chemical means C12Q1/04; colony counters C12M1/34; concentration of particle suspensions in general G01N15/06) · CPC title

• C12M41/36

  of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404) · CPC title

• A61B5/0531

  Measuring skin impedance · CPC title