Chip for measuring number of microbe, and apparatus for measuring number of microbe using the same

Patent metadata

Field	Value
Publication number	US-9500613-B2
Application number	US-201214233251-A
Country	US
Kind code	B2
Filing date	Jul 26, 2012
Priority date	Aug 5, 2011
Publication date	Nov 22, 2016
Grant date	Nov 22, 2016

Abstract

A chip that measures a number of microbes includes a chip main body in the form of a long plate, and a measuring electrode provided on a first end side in the longitudinal direction of the surface of the chip main body and that is immersed in a measurement liquid. A connecting electrode is connected to the measuring electrode and is provided on a second end side in the longitudinal direction of the surface of the chip main body. Ground electrodes are provided on the second end side in the longitudinal direction of the surface of the chip main body. Conductive patterns provided to the outer peripheral portion of the measuring electrode are connected to the ground electrodes.

First claim

The invention claimed is:

1. A chip for measuring a number of microbes, comprising:
a chip main body in a form of a long plate;
a measuring electrode provided on a first end side in a longitudinal direction of a surface of the chip main body, the measuring electrode being immersed in a measurement liquid and having two electrodes positioned opposite each other;
a connecting electrode connected to the measuring electrode and provided on a second end side on an opposite side from the first end in the longitudinal direction of the surface of the chip main body;
a ground electrode provided on the second end side of the surface of the chip main body; and
a conductive pattern connected to the ground electrode and provided to an outer peripheral portion of the measuring electrode,
wherein the ground electrode includes two electrodes each provided on an outer peripheral side of the connecting electrode so as to sandwich the connecting electrode.

2. The chip for a measuring number of microbes according to claim 1 ,
wherein a first end of the conductive pattern is connected to the ground electrode on the second end side in the longitudinal direction of the surface of the chip main body, and
a second end of the conductive pattern is provided around an outer periphery of the measuring electrode on the first end side in the longitudinal direction of the surface of the chip main body, and extends to the second end side in the longitudinal direction of the surface of the chip main body.

3. The chip for measuring a number of microbes according to claim 2 ,
wherein the second end of the conductive pattern is provided around the outer periphery of the measuring electrode on the first end side in the longitudinal direction of the surface of the chip main body, extends to the second end side in the longitudinal direction of the surface of the chip main body, and is connected to the ground electrode provided on the second end side in the longitudinal direction of the surface of the chip main body.

4. An apparatus for measuring a number of microbes in which the chip for measuring number of microbe according to claim 3 is used, comprising:
a container holder that holds a bottomed cylindrical container having an opening in its upper face, with the opening facing up;
a rotary driver that rotates a liquid stored inside the container held in the container holder, around the central axis of the container running in a substantially vertical direction;
an electrode insertion portion for inserting the chip for measuring number of microbe, through the opening in the container held in the container holder, at a position inside the container that is closer to the inner wall side than the central axis, and a position that is a specific distance away from the inner wall surface; and
a measurement section that measures microbes at the measurement electrode of the chip for measuring number of microbe inserted into the container by the electrode insertion portion,
wherein the electrode insertion portion has:
a connection terminal that is connected to the connecting electrode of the chip for measuring number of microbe; and
a ground terminal that is connected to the ground electrode of the chip for measuring number of microbe, and
the ground terminal and the ground electrode are connected before the connection terminal and the connecting electrode are connected.

5. The apparatus for measuring a number of microbes according to claim 4 ,
wherein the ground terminal of the electrode insertion portion is longer than the connection terminal.

6. The chip for measuring a number of microbes according to claim 2 ,
wherein the second end of the conductive pattern is provided around the outer periphery of the measuring electrode on the first end side in the longitudinal direction of the surface of the chip main body, extends to the second end side in the longitudinal direction of the surface of the chip main body, and is connected to a voltage application electrode provided on the second end side in the longitudinal direction of the surface of the chip main body.

7. An apparatus for measuring a number of microbes in which the chip for measuring number of microbe according to claim 6 is used, comprising:
a container holder that holds a bottomed cylindrical container having an opening in its upper face, with the opening facing up;
a rotary driver that rotates a liquid stored inside the container held in the container holder, around the central axis of the container running in a substantially vertical direction;
an electrode insertion portion for inserting the chip for measuring number of microbe, through the opening in the container held in the container holder, at a position inside the container that is closer to the inner wall side than the central axis, and a position that is a specific distance away from the inner wall surface; and
a measurement section that measures microbes at the measurement electrode of the chip for measuring number of microbe inserted into the container by the electrode insertion portion,
wherein the electrode insertion portion has:
a connection terminal that is connected to the connecting electrode of the chip for measuring number of microbe;
a ground terminal that is connected to the ground electrode of the chip for measuring number of microbe; and
a voltage application terminal that is connected to the voltage application electrode of the chip for measuring number of microbe, and
the ground terminal and the ground electrode are connected before the connection terminal and the connecting electrode are connected.

8. The apparatus for measuring a number of microbes according to claim 7 ,
wherein the connection terminal and the connecting electrode are connected before the voltage application terminal and the voltage application electrode are connected.

9. The apparatus for measuring a number of microbes according to claim 8 ,
wherein the ground terminal of the electrode insertion portion is longer than the connection terminal, and the connection terminal is longer than the voltage application terminal.

10. The chip for measuring a number of microbes according to claim 1 ,
wherein the two electrodes of the measuring electrode h include mutually opposing first and second measuring electrodes on the first end side in the longitudinal direction of the surface of the chip main body, and
the first and second measuring electrodes have mutually opposing comb electrodes formed a specific distance apart.

11. The chip for measuring a number of microbes according to claim 1 ,
wherein the connection pattern that connects the connecting electrode and the measuring electrode is covered by a covering body between the first end and second end in the longitudinal direction of the surface of the chip main body.

12. An apparatus for measuring a number of microbes in which the chip for measuring number of microbe according to claim 1 is used, comprising:
a container holder that holds a bottomed cylindrical container having an opening in its upper face, with the opening facing up;
a rotary driver that rotates a liquid stored inside the container held in the container holder, around the central axis of the container running in a substantially vertical direction;
an electrode insertion portion for inserting the chip for measuring number of microbe, through the opening in the container held in the container holder, at a position inside the container that is closer to the inner wall side than the central axis, and a position that is a specific distance away from the inner wall surface; and
a measurement section that measures microbes at the measurement electrode of the chip

Assignees

• Takeshita Toshiaki
• Kita Junichi
• Morita Hidenori
• Oouchi Kazufumi
• Panasonic Healthcare Holdings Co Ltd

Inventors

• Takeshita Toshiaki
• Kita Junichi
• Morita Hidenori
• Oouchi Kazufumi

Classifications

• G01N27/02 Primary

  by investigating impedance · CPC title

• G01N2015/0065

  Physics · mapped topic

• C12M41/36

  of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404) · CPC title

• G01N2035/00495

  Centrifuges · CPC title

• G01N15/0656 Primary

  using electric, e.g. electrostatic methods or magnetic methods (by investigating individual particles G01N15/1031, G01N15/12) · CPC title