Automated system for sample preparation and analysis

Patent metadata

Field	Value
Publication number	US-10088460-B2
Application number	US-201113882393-A
Country	US
Kind code	B2
Filing date	Oct 28, 2011
Priority date	Oct 29, 2010
Publication date	Oct 2, 2018
Grant date	Oct 2, 2018

Abstract

A sample preparation and analysis system. The system 10 includes a sample preparation system 12 and a sample analysis system 14 . The sample preparation system 12 prepares samples in accordance with an assay that is selected from a database containing a plurality of unique assays. The sample analysis system 14 includes an analyzer 110 that is dynamically reconfigurable based on the selected assay so as to analyze the prepared sample in accordance with that selected assay. A data communication link 27 communicates data from the sample preparation system 12 to the sample analysis system 14 to reconfigure the analyzer 110 in accordance with the selected assay.

First claim

What is claimed is:

1. A sample preparation and analysis system, comprising:
non-transitory computer readable memory and/or mass storage including:
a database and one or more data structures that alone or together contain stored information pertaining to a plurality of unique assays including the types of assays that the system may run, one or more sets of sample preparation operations corresponding to each of the assays, and one or more sets of sample analysis information corresponding to each of the assay;
at least one preparation control application; and
at least one sample analysis application;

a sample preparation system comprising:
a matrix interference removal station comprising a centrifuge; and
a sample preparation controller coupled to at least one of the database and the one or more data structures, wherein the at least one preparation control application comprises sample preparation instructions to cause the sample preparation controller to cause the sample preparation system to prepare a sample using one or more selected solvents and/or reagents by executing a selected one of the sets of sample preparation operations corresponding to a selected assay;

a sample analysis system comprising:
an analyzer comprising a mass spectrometer; and
a sample analysis controller, wherein the at least one sample analysis application comprises sample analysis instructions to cause the sample analysis controller to cause the sample analysis system to analyze the prepared sample using the analyzer by executing a selected one of the sets of sample analysis information corresponding to the selected assay; and

a data communication link coupled between the sample preparation system and the sample analysis system, wherein the at least one preparation control application comprises program instructions to cause the sample preparation controller to communicate data over the data communication link to the sample analysis controller that causes the sample analysis controller to configure or reconfigure the analyzer in accordance with the selected assay,
wherein the matrix interference removal station and the mass spectrometer are encompassed within a same housing.

2. The sample preparation and analysis system of claim 1 , wherein the communicated data are stored in at least one of the database and the one or more data structures and includes at least one of an identification of the selected assay, an identification of the volume of the prepared sample, an identification for the prepared sample, an identification of one or more analytes to be analyzed, or combinations thereof.

3. The sample preparation and analysis system of claim 1 , wherein the at least one sample analysis application comprises program instructions to cause the sample analysis system to communicate result data over the data communication link to the sample preparation system, the result data representing at least one of a result of the analysis, an identification for the prepared sample that the result of the analysis is associated with, a status of one or more components comprising the sample analysis system, a status of the analysis of the prepared sample, or combinations thereof.

4. The sample preparation and analysis system of claim 1 , wherein the data communication link includes at least one of a high level data link or a low level data link.

5. The sample preparation and analysis system of claim 4 , wherein the high level data link is a TCP/IP data link.

6. The sample preparation and analysis system of claim 4 , wherein the low level data link includes an I/O data link.

7. The sample preparation and analysis system of claim 1 , wherein the data communication link electrically couples the first controller and the second controller.

8. The sample preparation and analysis system of claim 1 , wherein the sample preparation system includes an injector pipette assembly and the sample analysis system includes an injector port, and wherein the data communication link includes a low level data link that connects the injector pipette assembly and the injector port.

9. The sample preparation and analysis system of claim 1 , wherein the analyzer includes a liquid chromatography station.

10. The sample preparation and analysis system of claim 9 , wherein the at least one preparation control application comprises program instructions to cause the sample preparation controller to communicate data over the data communication link to the sample analysis controller that causes the sample analysis controller to configure or reconfigure the analyzer by changing one or more settings of the liquid chromatography station, mass spectrometer, or both in accordance with the selected assay.

11. A method of preparing and analyzing a sample, comprising:
providing one or more databases or data structures having stored information pertaining to a plurality of unique assays, the information including a respective set of sample preparation operations and a respective set of sample analysis information corresponding to each of assays;
preparing a sample taken from a specimen in accordance with a selected one of the sets of sample preparation operations corresponding to a selected assay using a sample preparation system that includes a plurality of solvents and/or reagents and a matrix interference removal station comprising a centrifuge;
communicating configuration data over a data communication link between the sample preparation system and a sample analysis system including an analyzer comprising a mass spectrometer that is within a same housing as the matrix interference removal station, the data being used to configure or reconfigure the analyzer in accordance with the selected assay; and
analyzing the prepared sample with the analyzer in accordance with a selected one of the sets of sample analysis information corresponding to the selected assay.

12. The method of claim 11 , wherein the configuration data represents at least one of an identification of the selected assay, an identification of the volume of the prepared sample, an identification for the prepared sample, an identification of one or more analytes to be analyzed, or combinations thereof.

13. The method of claim 11 , further comprising communicating result data between the sample analysis system and the sample preparation system over the data communication link, wherein the result data represents at least one of a result of the analysis, a status of one or more components comprising the sample analysis system, or combinations thereof.

14. The method of claim 11 , wherein the data is communicated over a data communication link that includes at least one of a high level data link or a low level data link.

15. The method of claim 14 , wherein the high level data link is a TCP/IP data link.

16. The method of claim 14 , wherein the low level data link includes an I/O data link.

17. The method of claim 11 further comprising:
transporting the prepared sample from the sample preparation system to the sample analysis system.

18. The method of claim 11 further comprising:
sequencing the sample for preparation in accordance with the selected assay; and
sequencing the analysis of the prepared sample in accordance with the selected assay.

19. The method of claim 11 further comprising:
receiving a specimen and acquiring the sample from the specimen.

20. The method of claim 11 wherein the analyzing of the prepared sample with the analyzer comprises:
generating at least one sample fraction using a liquid chromatograp

Assignees

• Dewitte Robert
• Siidorov Juhani
• Nuotio Vesa
• Salminen Raimo
• Vehkomaki Jarmo
• Saukkonen Jukka
• Ostman Bill
• Senteno Joseph M
• Brann Iii John Edward
• Herman Joseph L
• Zonderman Jeffrey A
• Olney Terry N
• Thermo Fisher Scientific Oy

Inventors

• Dewitte Robert
• Siidorov Juhani
• Nuotio Vesa
• Salminen Raimo
• Vehkomaki Jarmo
• Saukkonen Jukka
• Ostman Bill
• Senteno Joseph M
• Brann Iii John Edward
• Herman Joseph L
• Zonderman Jeffrey A
• Olney Terry N

Classifications

• G01N35/08

  using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis · CPC title

• G01N30/06 Primary

  Preparation · CPC title

• G01N30/7233 Primary

  interfaced to liquid or supercritical fluid chromatograph (interfaces in general for introducing or extracting samples to be analysed with specially adapted mass spectrometer, see H01J49/04) · CPC title

• G01N2030/027

  Liquid chromatography · CPC title

• H01J49/0031

  Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence) · CPC title