Compositions comprising a polypeptide having cellulolytic enhancing activity and a nitrogen-containing compound and uses thereof

Patent metadata

Field	Value
Publication number	US-10087478-B2
Application number	US-201615134757-A
Country	US
Kind code	B2
Filing date	Apr 21, 2016
Priority date	Aug 12, 2010
Publication date	Oct 2, 2018
Grant date	Oct 2, 2018

Abstract

The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a nitrogen-containing compound. The present invention also relates to methods of using the compositions.

First claim

What is claimed is:

1. A method for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material with an enzyme composition comprising: (i) a GH61 polypeptide having cellulolytic enhancing activity and (ii) a nitrogen-containing compound, wherein the combination of the GH61 polypeptide having cellulolytic enhancing activity and the nitrogen-containing compound enhances hydrolysis of a cellulosic material by a cellulolytic enzyme compared to the GH61 polypeptide alone or the nitrogen-containing compound alone, and wherein the nitrogen-containing compound is a compound of formula (I) or (II-A):

wherein R 1 and R 2 are each independently hydrogen, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteraralkyl; or wherein R 1 and R 2 together with the attached carbon form an optionally substituted aromatic or non-aromatic ring;
wherein R 3 and R 4 are each independently hydrogen, an oxygen radical, —C(O)R 5 , —C(O)NHR 6 , —C(O)OR 7 , —SO 2 R 8 , —OR 9 , or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteraralkyl; or wherein R 3 and R 4 , R 3 and R 1 , or R 4 and R 2 together with the attached nitrogen form an optionally substituted aromatic or non-aromatic ring;
wherein R 9A , R 9B , and R 9C are independently hydrogen, halogen, —OH, —OR 10 , —CN, —NO 2 , —N(R 11 )(R 12 ), —C(O)R 13 , —C(O)OR 14 , —C(O)NHR 15 , —OC(O)R 16 , —NHC(O)R 17 , —OC(O)OR 18 , —NHC(O)OR 19 , —OC(O)NHR 20 , —NHC(O)NHR 21 , —SO 2 R 22 , —SO 2 N(R 23 )(R 24 ), —SR 25 , or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl; and wherein two of R 9A , R 9B , and R 9C may together form an optionally substituted fused ring; and
wherein R 10 , R 11 , R 12 , R 13 , R 14 , R 15 , R 16 , R 17 , R 18 , R 19 , R 20 , R 21 , R 22 , R 23 , R 24 , and R 25 are independently hydrogen, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
or a salt or solvate thereof;
or (II-3): 2,2,6,6-tetramethyl-1-piperidinyloxy or (II-6): maleamic acid; or a salt or solvate thereof; and
wherein the combination of the GH61 polypeptide having cellulolytic enhancing activity and the nitrogen-containing compound enhances hydrolysis of the cellulosic material by the enzyme composition according to the following formula with a ratio greater than 1:

GH
⁢

⁢
61
⁢

⁢
effect

=

%
⁢

⁢

conversion

(

+
GH

⁢

⁢
61

+

nitrogen
⁢

-

⁢
containing
⁢

⁢
compound

)

%
⁢

⁢

conversion

(

no
⁢

⁢
GH
⁢

⁢
61

+

nitrogen
⁢

-

⁢
containing
⁢

⁢
compound

)

;

(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation.

2. The method of claim 1 , wherein the cellulosic material is pretreated.

3. The method of claim 1 , wherein the enzyme composition further comprises one or more enzymes selected from the group consisting of a cellulase, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.

4. The method of claim 3 , wherein the cellulase is one or more enzymes selected from the group consisting of an endoglucanase, a cellobiohydrolase, and a beta-glucosidase.

5. The method of claim 3 , wherein the hemicellulase is one or more enzymes selected from the group consisting of a xylanase, an acetyxylan esterase, a feruloyl esterase, an arabinofuranosidase, a xylosidase, and a glucuronidase.

6. The method of claim 1 , wherein steps (a) and (b) are performed simultaneously in a simultaneous saccharification and fermentation.

7. The method of claim 1 , wherein the fermentation product is an alcohol, an alkane, a cycloalkane, an alkene, an amino acid, a gas, isoprene, a ketone, an organic acid, or polyketide.

8. The method of claim 1 , wherein the nitrogen-containing compound comprises an amine, imine, hydroxylamine, or nitroxide moiety.

9. The method of claim 1 , wherein the nitrogen-containing compound is selected from the group consisting of: (I-1): acetone oxime; (I-2): violuric acid; (I-3): pyridine-2-aldo

Assignees

• Novozymes Inc

Inventors

• Quinlan Jason
• Xu Feng
• Sweeney Matthew

Classifications

• C12P19/12

  Disaccharides · CPC title

• C12P2203/00

  Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source · CPC title

• C12P5/026

  Unsaturated compounds, i.e. alkenes, alkynes or allenes · CPC title

• C12P13/04

  Alpha- or beta- amino acids {(other amino acids C12P13/005)} · CPC title

• C12P5/02

  acyclic {(C12P5/007 takes precedence)} · CPC title