Reducing levels of nicotinic alkaloids in plants

Patent metadata

Field	Value
Publication number	US-10085478-B2
Application number	US-201715828868-A
Country	US
Kind code	B2
Filing date	Dec 1, 2017
Priority date	Feb 28, 2005
Publication date	Oct 2, 2018
Grant date	Oct 2, 2018

Abstract

Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants.

First claim

What is claimed is:

1. A tobacco plant produced by a method comprising:
(a) mutagenizing a plurality of tobacco plant cells to comprise one or more mutations in the nucleotide sequence set forth in SEQ ID NO: 3, thereby producing a plurality of mutagenized cells comprising the one or more mutations in the nucleotide sequence set forth in SEQ ID NO: 3;
(b) screening plants generated from the mutagenized cells comprising the one or more mutations in the nucleotide sequence set forth in SEQ ID NO: 3 for reduced expression of NBB1; and
(c) selecting one or more of the progeny comprising the one or more mutations in the nucleotide sequence set forth in SEQ ID NO: 3, wherein the progeny has reduced NBB1 expression and reduced nicotine content as compared to a control tobacco plant,
thereby resulting in a tobacco plant having reduced NBB1 expression and reduced nicotine content as compared to a control tobacco plant.

2. A tobacco product produced from the tobacco plant of claim 1 , wherein:
(a) the tobacco product is selected from the group consisting of smoking cessation products, cigarettes, cigarette tobacco, cigars, cigar tobacco, snus, pipe tobacco, and chewing tobacco, and
(b) the tobacco product comprises the mutagenized cells of claim 1 .

3. A product produced from the tobacco plant of claim 1 , wherein:
(a) the product is selected from the group consisting of a food product, food ingredient, feed product, feed ingredient, nutritional supplement, and biofuel, and
(b) the product comprises the mutagenized cells of claim 1 .

Assignees

• 22Nd Century Ltd Llc

Inventors

• Hashimoto Takashi
• Kato Akira

Classifications

• C12N5/04

  Plant cells or tissues {(culture media C12N5/0025)} · CPC title

• A24D1/00

  Cigars; Cigarettes · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12N15/8218

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title

• C12N15/8243 Primary

  involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine · CPC title