Reducing levels of nicotinic alkaloids in plants

Patent metadata

Field	Value
Publication number	US-9834780-B2
Application number	US-201514685361-A
Country	US
Kind code	B2
Filing date	Apr 13, 2015
Priority date	Feb 28, 2005
Publication date	Dec 5, 2017
Grant date	Dec 5, 2017

Abstract

Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants.

First claim

What is claimed is:

1. A method for producing a tobacco plant cell having suppression of a gene product encoded by SEQ ID NO: 3, comprising genetically engineering a population of plant cells to suppress production of the gene product encoded by SEQ ID NO: 3, comprising introducing one or more mutations into the region of SEQ ID NO: 3
that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO: 4, wherein the plant cells exhibit reduced expression of the gene product as compared to a non-transformed control cell.

2. A plant cell produced by the method of claim 1 , wherein the plant cell comprises in its genome the one or more mutations and exhibits reduced expression of the gene product encoded by SEQ ID NO: 3 as compared to a non-transformed control cell.

3. A plant having suppression of the gene product encoded by SEQ ID NO: 3 and a reduced nicotine level, as compared to a control plant, produced from the plant cell of claim 2 .

4. A product comprising the plant of claim 3 , and having a reduced level of nicotine as compared to a product produced from a control plant, wherein the product is selected from the group consisting of a tobacco product, food product, food ingredient, feed product, feed ingredient, nutritional supplement, and biofuel.

5. The tobacco product of claim 4 , wherein the product is selected from the group consisting of smoking cessation products, cigarettes, cigarette tobacco, cigars, cigar tobacco, snus, pipe tobacco, and chewing tobacco.

6. The method of claim 1 , wherein the genetic engineering comprises introducing into the population of cells a reagent comprising a recombinagenic oligonucleobase to introduce said mutations.

7. Seeds from the plant of claim 3 or progeny plant thereof, wherein the seeds comprise in their genomes the one or more mutations.

Assignees

• 22Nd Century Ltd Llc

Inventors

• Hashimoto Takashi
• Kato Akira

Classifications

• C12N15/8243 Primary

  involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine · CPC title

• A24B13/00

  Tobacco for pipes, for cigars, e.g. cigar inserts, or for cigarettes; Chewing tobacco; Snuff (reconstituted tobacco products A24B3/14) · CPC title

• A24F47/00

  Smokers' requisites not otherwise provided for · CPC title

• C12N15/8218

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title

• A24D1/00

  Cigars; Cigarettes · CPC title