High Fidelity Restriction Endonucleases
US-2024352437-A1 · Oct 24, 2024 · US
Thioesterases and their use
US10066219B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10066219-B2 |
| Application number | US-201113824060-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 26, 2011 |
| Priority date | Sep 24, 2010 |
| Publication date | Sep 4, 2018 |
| Grant date | Sep 4, 2018 |
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- Title
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Abstract
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Also disclosed are compositions comprising thioesters produced by the methods disclosed herein.
First claim
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The invention claimed is: 1. A method of producing an enzyme having thioesterase activity comprising: inserting at least one of the nucleotide sequences of SEQ ID NOS: 1, 3, 5 or 7 into an expression vector; transfecting an isolated host cell with said vector, and growing said transformed host cell in suitable culture conditions. 2. A method of producing thioesters comprising contacting at least one thioesterase enzyme comprising at least one of the amino acid sequences of SEQ ID NOS 2, 4, 6, or 8 and/or at least one isolated transfected host cell comprising at least one of the nucleotide sequences of SEQ ID NOS 1, 3, 5 or 7 with at least one suitable substrate, incubating the mixture, isolating the crude product containing the thioester and, purifying the crude product to obtain only the thioester. 3. The method of claim 2 , wherein the substrate comprises a C1-C8 short chain fatty acid coenzyme A (SCFA-CoA).
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Classifications
Preparation of sulfur-containing organic compounds · CPC title
Thioester hydrolases (3.1.2) · CPC title
Dairy flavours · CPC title
acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4) · CPC title
Enzymes other than milk clotting enzymes, e.g. lipase, beta-galactosidase · CPC title
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- What does patent US10066219B2 cover?
- Also disclosed are compositions comprising thioesters produced by the methods disclosed herein.
- Who is the assignee on this patent?
- Bhowmik Tarun, Broadbent Jeff, Welker Dennis, and 5 more
- What technology area does this patent fall under?
- Primary CPC classification C12N9/16. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Sep 04 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).