Xylose isomerase signature sequences

Patent metadata

Field	Value
Publication number	US-10053684-B2
Application number	US-201514745202-A
Country	US
Kind code	B2
Filing date	Jun 19, 2015
Priority date	Jul 24, 2012
Publication date	Aug 21, 2018
Grant date	Aug 21, 2018

Abstract

This disclosure relates to novel xylose isomerases and their uses, particularly in fermentation processes that employ xylose-containing media.

First claim

What is claimed is:

1. A vector comprising a nucleic acid which encodes a polypeptide comprising a xylose isomerase signature sequence, wherein the xylose isomerase signature sequence is selected from the group consisting of SEQ ID NO: 212, SEQ ID NO: 213 and SEQ ID NO: 214 or a combination thereof,
wherein for SEQ ID NO:212:
Amino acid residue 3 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine,
Amino acid residue 6 is Arginine or Lysine;
Amino acid residue 7 is any 2 of Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
Amino acid residue 9 is Tryptophan, Tyrosine or Phenylalanine;
Amino acid residue 10 is Aspartic Acid, Asparagine or Glycine;
Amino acid residue 11 is any 2 or 3 of Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
Amino acid residue 12 is Aspartic Acid, Asparagine, Glutamic Acid, Glycine or Threonine; and
Amino acid residue 13 is Alanine or Threonine;

wherein for SEQ ID NO:213:
Amino acid residue 3 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
Amino acid residue 6 is Arginine or Lysine;
Amino acid residue 7 is any 2 of Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
Amino acid residue 9 is Tryptophan, Tyrosine or Phenylalanine;
Amino acid residue 10 is Aspartic Acid, Asparagine or Glycine;
Amino acid residue 11 is any 3 of Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
Amino acid residue 12 is Alanine, Arginine, Asparagine, Aspartic acid, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine; and
Amino acid residue 13 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Serine, Threonine, Tryptophan, Tyrosine, or Valine; and

wherein for SEQ ID NO:214:
Amino acid residue 1 is Phenylalanine or Tryptophan;
Amino acid residue 4 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
Amino acid residue 6 is Leucine, Valine or Isoleucine;
Amino acid residue 7 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
Amino acid residue 8 is Aspartic Acid or Glutamic Acid;
Amino acid residue 11 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
Amino acid residue 12 is Threonine, Serine or Asparagine;
Amino acid residue 13 is Isoleucine or Valine; and
Amino acid residue 14 is Glutamic acid or Alanine.

2. The vector of claim 1 , further comprising on origin of replication and/or a promoter operably linked to the nucleic acid.

3. The vector of claim 2 , wherein the promoter is operable in yeast and/or filamentous fungi.

4. A host cell transformed with the vector of claim 1 .

5. The host cell of claim 4 , wherein the cell is selected from the group consisting of a prokaryotic cell, a bacterial cell and a eukaryotic cell.

6. A recombinant cell which expresses the polypeptide comprising a xylose isomerase signature sequence, wherein the xylose isomerase signature sequence is selected from the group consisting of SEQ ID NO: 212, SEQ ID NO: 213 and SEQ ID NO: 214 or a combination thereof,
wherein for SEQ ID NO:212:
Amino acid residue 3 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine,
Amino acid residue 6 is Arginine or Lysine;
Amino acid residue 7 is any 2 of Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
Amino acid residue 9 is Tryptophan, Tyrosine or Phenylalanine;
Amino acid residue 10 is Aspartic Acid, Asparagine or Glycine;
Amino acid residue 11 is any 2 or 3 of Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
Amino acid residue 12 is Aspartic Acid, Asparagine, Glutamic Acid, Glycine or Threonine; and
Amino acid residue 13 is Alanine or Threonine;

wherein for SEQ ID NO:213:
Amino acid residue 3 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
Amino acid residue 6 is Arginine or Lysine;
Amino acid residue 7 is any 2 of Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
Amino acid residue 9 is Tryptophan, Tyrosine or Phenylalanine;
Amino acid residue 10 is Aspartic Acid, Asparagine or Glycine;
Amino acid residue 11 is any 3 of Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
Amino acid residue 12 is Alanine, Arginine, Asparagine, Aspartic acid, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine; and
Amino acid residue 13 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Serine, Threonine, Tryptophan, Tyrosine, or Valine; and

wherein for SEQ ID NO:214:
Amino acid residue 1 is Phenylalanine or Tryptophan;
Amino acid residue 4 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
Amino acid residue 6 is Leucine, Valine or Isoleucine;
Amino acid residue 7 is Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, or Valine;
Amino acid residue 8 is Aspartic Acid or Glutamic Acid;
Amin

Assignees

• Bp Corp North America Inc

Inventors

• Nunn Jr David Neal
• Luginbuhl Peter
• Li Ling
• Burja Adam Martin
• Bartnek Jon Peter Flash

Classifications

• Y02E50/343

  Cross-Sectional Technologies · mapped topic

• C12P13/04

  Alpha- or beta- amino acids {(other amino acids C12P13/005)} · CPC title

• C12P35/06

  Cephalosporin C; Derivatives thereof · CPC title

• C12P7/54

  Acetic acid (vinegar C12J) · CPC title

• C12P7/48

  Tricarboxylic acids, e.g. citric acid · CPC title