Consumable cryopreserved cells transiently overexpressing gene(s) encoding drug transporter protein(s)

Patent metadata

Field	Value
Publication number	US-10047136-B2
Application number	US-201715688983-A
Country	US
Kind code	B2
Filing date	Aug 29, 2017
Priority date	Sep 11, 2012
Publication date	Aug 14, 2018
Grant date	Aug 14, 2018

Abstract

The present invention discloses cryopreserved recombinant cells for screening drug candidates that transiently overexpress one or more drug transporter proteins and/or drug metabolizing enzymes. Advantageously, such cells provide a cost-efficient consumable product that streamlines the process of screening whether drug candidates are substrates or inhibitors of drug transporter proteins and/or drug metabolizing enzymes.

First claim

What is claimed is:

1. A cryopreserved recombinant cell comprising one or more transiently overexpressed genes encoding a drug transporter protein, wherein activity of the drug transporter protein is detectable in a population of the cryopreserved recombinant cell following thaw from cryopreservation, wherein the cryopreserved recombinant cell is transiently transfected with the one or more genes by a method comprising electroporation, and wherein said one or more genes is OAT3 and wherein said cell is HEK293.

2. The recombinant cell of claim 1 , wherein said one or more genes is derived individually from human or an animal species selected from mouse, rat, guinea pigs, dog, and monkey.

3. The recombinant cell of claim 1 , wherein activity of the drug transporter protein is detectable in a population of said cell at least 24 hours post plating following thaw from cryopreservation.

4. The recombinant cell of claim 1 , wherein activity of the drug transporter protein is detectable in a population of said cell at least 48 hours post plating following thaw from cryopreservation.

5. The recombinant cell of claim 1 , wherein activity of the drug transporter protein is detectable in a population of said cell at least 72 hours post plating following thaw from cryopreservation.

6. A process of preparing cryopreserved transiently transfected recombinant cells, the process comprising:
transiently transfecting cells with one or more genes encoding a drug transporter protein to provide the transiently transfected recombinant cells; and
cryopreserving the transiently transfected recombinant cells within 48 hours of transfection, wherein the transient transfection of the cells comprises electroporation;
wherein said one or more genes is OAT3 and wherein said cell is HEK293.

7. The process of claim 6 , wherein the transiently transfected recombinant cells are cryopreserved at 30 minutes to 24 hours post transfection.

8. A cryopreserved transiently transfected recombinant cell prepared according to the process of claim 6 .

Assignees

• Corning Inc

Inventors

• Li Na
• Wang Jie
• Patten Christopher J

Classifications

• G08B25/08

  using communication transmission lines {(G08B13/19658, G08B21/0286, G08B25/016 take precedence)} · CPC title

• G08B25/001

  Alarm cancelling procedures or alarm forwarding decisions, e.g. based on absence of alarm confirmation · CPC title

• G05B2219/2642

  Domotique, domestic, home control, automation, smart house · CPC title

• G08B29/185

  Signal analysis techniques for reducing or preventing false alarms or for enhancing the reliability of the system · CPC title

• G05B15/02

  electric · CPC title