L-threonine and L-tryptophan producing bacteria strain and method of making same

Patent metadata

Field	Value
Publication number	US-10041099-B2
Application number	US-201314370500-A
Country	US
Kind code	B2
Filing date	Jan 7, 2013
Priority date	Jan 6, 2012
Publication date	Aug 7, 2018
Grant date	Aug 7, 2018

Abstract

The present invention relates to a microorganism able to produce L-threonine or L-tryptophan, and to a method for producing L-threonine or L-tryptophan by using same. More specifically, the present invention relates to: recombinant Escherichia coli which is more efficient in producing L-threonine or L-tryptophan by increasing the ability to produce ATP which is used as the most plentiful energy source in cells when producing L-threonine or L-tryptophan; and a method for producing L-threonine or L-tryptophan by using same.

First claim

The invention claimed is:

1. An L-threonine or L-tryptophan producing recombinant Escherichia coli strain, wherein the strain is modified to attenuate activity of at least two selected from the group consisting of: a protein YsaA having the amino acid sequence of SEQ ID NO: 2, a protein YdaS having the amino acid sequence of SEQ ID NO: 4, and a protein YbiX having the amino acid sequence of SEQ ID NO: 6, wherein the activity is attenuated by deletion of at least a gene encoding the protein YsaA, the protein YdaS, and/or the protein YbiX.

2. The L-threonine or L-tryptophan producing recombinant Escherichia coli strain according to claim 1 , wherein the protein YsaA is encoded by a polynucleotide sequence of SEQ ID NO: 1, the protein YdaS is encoded by a polynucleotide sequence of SEQ ID NO: 3, and the protein YbiX is encoded by a polynucleotide sequence of SEQ ID NO: 5.

3. The L-threonine or L-tryptophan producing recombinant Escherichia coli strain according to claim 1 , wherein the recombinant Escherichia coli strain is L-threonine producing Escherichia coli CA03-4257P (KCCM11243P).

4. The L-threonine or L-tryptophan producing recombinant Escherichia coli strain according to claim 1 , wherein the recombinant Escherichia coli strain is L-tryptophan producing Escherichia coli CA04-2002 (KCCM11245P).

5. A method for producing L-threonine or L-tryptophan, the method comprising culturing an L-threonine or L-tryptophan producing recombinant Escherichia coli strain, wherein the strain is modified to attenuate activity of at least two selected from the group consisting of a protein (YsaA) having the amino acid sequence of SEQ ID NO: 2, a protein (YdaS) having the amino acid sequence of SEQ ID NO: 4, and a protein (YbiX) having the amino acid sequence of SEQ ID NO: 6, wherein the activity is attenuated by deletion of at least a gene encoding the protein YsaA, protein YdaS, and/or the protein YbiX.

6. The method according to claim 5 , wherein the recombinant Escherichia coli strain is L-threonine producing Escherichia coli CA03-4257P (KCCM11243P).

7. The method according to claim 5 , wherein the recombinant Escherichia coli strain is L-tryptophan producing Escherichia coli CA04-2002 (KCCM11245P).

Assignees

• Cj Cheiljedang Corp

Inventors

• Cheong Ki Yong
• Lee Seok Myung
• Hwang Young Bin
• Lee Keun Cheol
• Lee Kwang Ho

Classifications

• C12P13/227 Primary

  Tryptophan · CPC title

• C12N9/0053

  acting on a heme group of donors (1.9) · CPC title

• Y02P20/59

  Biological synthesis; Biological purification · CPC title

• C07K14/245 Primary

  Escherichia (G) · CPC title

• C12Y114/11

  with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11) · CPC title