Microfluidic element for thoroughly mixing a liquid with a reagent

Patent metadata

Field	Value
Publication number	US-10031053-B2
Application number	US-201615208211-A
Country	US
Kind code	B2
Filing date	Jul 12, 2016
Priority date	Dec 13, 2007
Publication date	Jul 24, 2018
Grant date	Jul 24, 2018

Abstract

A microfluidic element for thoroughly mixing a liquid with a reagent used for the analysis of the liquid for an analyte contained therein and a method thereof are disclosed. The microfluidic element has a substrate and a channel structure. The channel structure includes an elongate mixing channel and an output channel. The mixing channel has an inlet opening and an outlet opening, and is implemented to mix the reagent contained therein with the liquid flowing through the inlet opening into the mixing channel. The outlet opening of the mixing channel is in fluid communication to the output channel. The outlet opening is positioned closer to the middle of the length of the mixing channel than the inlet opening.

First claim

What is claimed is:

1. A microfluidic element for thoroughly mixing a liquid with a reagent used for analyzing the liquid for an analyte contained therein, the microfluidic element comprising:
a substrate; and
a channel structure that includes an elongate mixing channel and an output channel, the channel structure being provided by the substrate, and wherein:
the mixing channel has a directly adjoining inlet opening and an outlet opening, the outlet opening being in fluid communication with the output channel,
the outlet opening is located closer to the middle of the length of the mixing channel than the inlet opening,
the mixing channel has a feed section between the inlet opening and the outlet opening and a complementary section, downstream from the outlet opening in the flow direction and opposite to the inlet opening, and
the mixing channel is adapted for mixing a reagent contained therein when the liquid flows through the inlet opening into the mixing channel.

2. The microfluidic element according to claim 1 , wherein the microfluidic element further comprises a cover layer, and the channel structure is enclosed by the substrate and cover layer.

3. The microfluidic element according to claim 1 , wherein parts of the channel structure are defined by the substrate.

4. The microfluidic element according to claim 1 , wherein the microfluidic element is a test carrier.

5. The microfluidic element according to claim 1 , wherein the channel structure is a sample analysis channel which includes a measuring zone.

6. The microfluidic element according to claim 1 , wherein the microfluidic element is rotatable about an axis of rotation.

7. The microfluidic element according to claim 6 , wherein the mixing channel is so shaped that the distance of the outlet opening from the axis of rotation is greater than the distance of the inlet opening from the axis of rotation.

8. The microfluidic element according to claim 1 , further comprising a capillary stop which forms a flow resistance for the liquid flowing from the mixing channel into the output channel in such a manner that spontaneous emptying of the mixing channel into the output channel is prevented until the flow resistance is overcome by an external force.

9. The microfluidic element according to claim 8 , wherein the external force is a centrifugal force generated by rotation of the microfluidic element and/or a pressure force which acts on the liquid in the mixing channel.

10. The microfluidic element according to claim 8 , wherein the capillary stop is formed by a geometric valve, which includes a primary section and a secondary section downstream from the primary section in the flow direction, the cross-sectional area of the primary section being smaller than the cross-sectional area of the secondary section.

11. The microfluidic element according to claim 8 , wherein the capillary stop includes a channel section having at least one hydrophobic channel wall.

12. The microfluidic element according to claim 1 , wherein the reagent is contained in the mixing channel in dried form.

13. The microfluidic element according to claim 1 , wherein the reagent is contained in the mixing channel in lyophilized form.

14. The microfluidic element according to claim 1 , wherein the outlet opening is positioned from the middle of the total length of the mixing channel at a distance that is at most 20% of the total length of the mixing channel.

15. The microfluidic element according to claim 1 , wherein the inlet opening is positioned from one end of the mixing channel at a distance that is at most 20% of the total length of the mixing channel.

16. The microfluidic element according to claim 1 , wherein the volume of the mixing channel is larger than the volume of the output channel.

17. The microfluidic element according to claim 1 , wherein the mixing channel has a rectangular cross-section.

18. The microfluidic element according to claim 1 , wherein the mixing channel has a length that is at least ten (10) times as large as the greater cross-sectional dimension of its cross-sectional area.

Assignees

• Roche Diagnostics Operations Inc
• Roche Diagnostics Operations Inc

Inventors

• Boehm Christoph

Classifications

• B01L2400/0409

  centrifugal forces · CPC title

• B01L2300/087

  Multiple sequential chambers · CPC title

• B01F15/0233

  Operations & Transport · mapped topic

• B01L3/50273 Primary

  characterised by the means or forces applied to move the fluids · CPC title

• B01F13/0059

  Operations & Transport · mapped topic