Microfluidic element for thoroughly mixing a liquid with a reagent

Patent metadata

Field	Value
Publication number	US-9417164-B2
Application number	US-201313803747-A
Country	US
Kind code	B2
Filing date	Mar 14, 2013
Priority date	Dec 13, 2007
Publication date	Aug 16, 2016
Grant date	Aug 16, 2016

Abstract

A microfluidic element for thoroughly mixing a liquid with a reagent used for the analysis of the liquid for an analyte contained therein and a method thereof are disclosed. The microfluidic element has a substrate and a channel structure. The channel structure includes an elongate mixing channel and an output channel. The mixing channel has an inlet opening and an outlet opening, and is implemented to mix the reagent contained therein with the liquid flowing through the inlet opening into the mixing channel. The outlet opening of the mixing channel is in fluid communication to the output channel. The outlet opening is positioned closer to the middle of the length of the mixing channel than the inlet opening.

First claim

What is claimed is:

1. A method for providing a homogeneous thoroughly mixed liquid comprising:
providing a microfluidic element having a substrate and a channel structure, wherein the channel structure includes an elongate mixing channel and an output channel, wherein the mixing channel has a directly adjoining inlet opening and an outlet opening, the outlet opening being in fluid communication with the output channel, wherein the outlet opening is located closer to the middle of the length of the mixing channel than the inlet opening, and, wherein the mixing channel has a feed section between the inlet opening and the outlet opening and a complementary section, downstream from the outlet opening in the flow direction and opposite to the inlet opening;
receiving in the mixing channel a flow of liquid exteriorly applied directly to the inlet opening;
dissolving a reagent contained in the mixing channel; and
exerting a force on the liquid in the mixing channel to:
allow the liquid to flow into the output channel through the outlet opening of the mixing channel so that thorough mixing of the liquid and the reagent occur, and
flow partial volumes from the feed section and the complementary section of the mixing channel through the outlet opening into the output channel such that mixing of the two liquid partial volumes is supported by exertion of the force on the liquid.

2. The method according to claim 1 , wherein the microfluidic element further comprises a cover layer.

3. The method according to claim 2 , wherein the channel structure is enclosed by the substrate and cover layer.

4. The method according to claim 1 , wherein the exerted force is a centrifugal force which is generated by rotating the microfluidic element.

5. The method according to claim 1 , wherein the microfluidic element is a test carrier.

6. The method according to claim 5 , wherein the test carrier comprises a second channel in fluid communication with a collection chamber, wherein the second channel includes an inlet opening, a mixing channel, an output channel, and a capillary stop positioned between them, the method further comprising receiving an additional liquid.

7. The method according to claim 6 , wherein the additional liquid is a buffer solution.

8. The method according to claim 6 , wherein the second channel is a sample analysis channel.

9. The method according to claim 3 , wherein the channel structure is a sample analysis channel which comprises a sample inlet opening and a measuring zone.

10. The method according to claim 9 , further comprising allowing the liquid to flow into the measuring zone and determining a measured variable characteristic for an analyte.

11. The method according to claim 10 , wherein the analyte is detected optically.

12. The method according to claim 1 , wherein the exerted force is a pressure force which is implemented by generating an overpressure or partial vacuum within the carrier.

13. The method according to claim 1 , wherein the reagent is in dried form.

14. The method according to claim 13 , wherein the reagent is in lyophilized form.

15. The method according to claim 1 , wherein the microfluidic element further comprises a capillary stop which forms a flow resistance for the liquid flowing from the mixing channel into the output channel in such a manner that spontaneous emptying of the mixing channel into the output channel is prevented until the flow resistance is overcome by an external force.

16. The method according to claim 1 , wherein the concentration in the liquid partial volume from the feed section and the concentration in the liquid partial volume from the complementary section supplement one another mutually or complementary such that an uniform thorough mixing occurs upon entrance in the output channel.

17. The method according to claim 1 , wherein a thorough mixing occurs upon entrance in the output channel and wherein the concentration in the liquid partial volume in the feed section differs from the concentration in the liquid partial volume in the complementary section.

18. The method according to claim 1 , wherein upon flowing into the output channel the amount of the liquid partial volume from the feed section equals the amount of the liquid partial volume of the complementary section such that a homogenous mixing is effected.

19. The method according to claim 1 , wherein the outlet opening is positioned at most 20% of the length of the mixing channel distant from the middle of the length of the mixing channel.

20. The method according to claim 1 , wherein the inlet opening is at most 20% of the length of the mixing channel distant from one end of the mixing channel.

21. The method according to claim 4 , wherein the channel structure comprises a collection chamber connected to the outlet channel and a measurement zone connected to the collection chamber, and the method further comprises stopping or slowing the rotating of the microfluidic element to draw the fluid into the measuring zone from the collection chamber.

22. A method for providing a homogeneous thoroughly mixed liquid comprising:
providing a microfluidic element having a substrate and a channel structure, wherein the channel structure includes an elongate mixing channel and an output channel, wherein the mixing channel has a directly adjoining inlet opening and an outlet opening in fluid communication with the output channel, wherein the outlet opening is located closer to the middle of the length of the mixing channel than the inlet opening, and, wherein the mixing channel has a feed section between the inlet opening and the outlet opening and a complementary section, downstream from the outlet opening in the flow direction and opposite to the inlet opening;
receiving in the mixing channel a flow of liquid exteriorly applied directly to the inlet opening, in which said liquid self fills the mixing channel via capillary action and dissolves a reagent contained in the mixing channel; and
directly following the receiving in the mixing channel the flow of liquid, exerting a force on the liquid in the mixing channel necessary to overcome flow resistance to the output channel, wherein said force causes partial volumes of the liquid to simultaneously flow from the feed section and the complementary section of the mixing channel through the outlet opening into the output channel which results in thorough mixing of the liquid and the dissolved reagent via the mixing of the two liquid partial volumes that is supported by exertion of the force on the liquid.

Assignees

• Roche Diagnostics Operations Inc

Inventors

• Boehm Christoph

Classifications

• B01L3/50273 Primary

  characterised by the means or forces applied to move the fluids · CPC title

• B01L2400/0409

  centrifugal forces · CPC title

• Y10T436/25

  including sample preparation · CPC title

• B01L2300/087

  Multiple sequential chambers · CPC title

• G01N21/07

  Centrifugal type cuvettes (G01N21/09 takes precedence) · CPC title