Sample processing apparatus and method

Patent metadata

Field	Value
Publication number	US-10006086-B2
Application number	US-201615210075-A
Country	US
Kind code	B2
Filing date	Jul 14, 2016
Priority date	Oct 2, 2009
Publication date	Jun 26, 2018
Grant date	Jun 26, 2018

Abstract

The invention relates to a sample processing apparatus comprising a holder for a microtiter plate comprising a plurality of microwells, optical measurement unit for measuring optical responses of samples dosed to the microwells, and a computing unit configured to analyze the optical responses in order to detect dosing failures in said plurality of microwells, and if a dosing failure has been detected in one or more of the microwells, to communicate the existence of the dosing failure to a user of the apparatus through signaling means or to store data indicative of the dosing failure to data storage means for further use. In particular, the invention relates to detecting dosing failures in before, during and after a PCR process.

First claim

The invention claimed is:

1. A solution set for a Polymerase Chain Reaction (PCR) assay, comprising
a) a first reagent solution comprising at least one substance required for performing said PCR assay,
b) a second reagent solution comprising at least one other substance required for performing said PCR assay,

wherein said first reagent solution is provided with a first colorant having a first color, said second reagent solution is provided with a second colorant having a second color different from said first color, and said first and second reagent solutions are capable of forming, on mixing, a mixed solution having, due to said first and second colorants, a third color different from said first and second colors, wherein said colors are capable of being detected visually.

2. The solution set according to claim 1 , wherein at least one of said reagent solutions is a sample solution for receiving a template to be amplified.

3. The solution set according to claim 1 , wherein at least one of said reagent solutions contains one or more of the following: a polymerase, a primer, ions or dNTPs, and/or a fluorescent quantitative PCR (qPCR) dye.

4. The solution set according to claim 1 , wherein at least one of said reagent solutions is an elution buffer or a dilution buffer.

5. The solution set according to claim 1 , wherein said first and/or second colorants are selected from the group of: Quinoline yellow, Xylene cyanol, Brilliant Blue, Patent Blue, Indigocarmine, Acid Red 1, m-Cresol Purple, Cresol Red, Neutral Red, Bromocresol Green, Acid Violet 5, Bromo phenol blue, and Orange G.

6. The solution set according to claim 1 , wherein the concentration of said colorants in respective reagent solutions corresponds to absorbances of said reagent solutions at their maximum absorption wavelengths of 0.01-0.5 when used in said PCR assay.

7. The solution set according to claim 1 , wherein said first reagent solution and/or said second reagent solution is a concentrate.

8. The solution set according to claim 1 , wherein said first reagent solution and said second reagent solution are capable of forming a transparent or translucent mixed solution suitable for quantitative PCR (qPCR).

9. The solution set according to claim 1 , wherein said first reagent solution is selected from: a polymerase solution or a polymerase buffer; and wherein said second reagent solution is selected from a composition comprising a primer, a probe and/or a sample.

10. The solution set according to claim 1 , wherein said mixed solution comprises a fluorescent agent and wherein the absorbance peaks of any of said colorants do not overlap with the emission or excitation wavelength of said fluorescent agent.

11. The solution set according to claim 1 , wherein said mixed solution comprises a fluorescent agent and the total absorbance of said mixed solution at the emission or excitation wavelength of said fluorescent agent is less than 0.1.

12. The solution set according to claim 1 , wherein at least one of said reagent solutions is a PCR master mix.

13. The solution set according to claim 12 , wherein said PCR master mix is a qPCR master mix.

Assignees

• Thermo Fisher Scientific Baltics Uab

Inventors

• Tuunanen Jukka
• Kurkela Jaako
• Eklin Katja

Classifications

• G01N21/251

  Colorimeters; Construction thereof · CPC title

• C12Q1/686

  Polymerase chain reaction [PCR] · CPC title

• G01N21/29

  using visual detection (G01N21/31 takes precedence) · CPC title

• G01N21/78

  producing a change of colour · CPC title

• G01N21/6428

  Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53) · CPC title